presentación[1].ppt
TRANSCRIPT
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ESTUDIO DEL EFECTO BIOCONSERVANTE Y/O PROBITICO DEL Enterococcus faecium Y NISINA EN FILETES DE DORADA.UNIVERSIDAD POLITCNICA DE VALENCIAEscuela Tcnica Superior del Medio Rural y EnologaRealizado por:
Mara del Mar Casas MorenoDirigido por:
M Jess Pagn Moreno Ral Grau MelValencia, Septiembre 2009
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Introduccin.
Satisfacer la demanda de los consumidoresINDUSTRIA ALIMENTARIA Naturales: menor presencia de conservantes u otros aditivos artificiales. Seguros pero mnimamente procesados. Nutritivos Gran vida til. Usos de las bacterias cido lcticas PROBITICO BIOCONSERVACIN. Efecto conservante por la produccin de diferentes metabolitos Competencia por nutrientes con la flora alterante/patgena.cido lcticoPerxido de hidrogenoBACTERIOCINASetc. Alta calidad Extensin de la vida til Fciles de preparar Incremento de la seguridadMicrobiota natural y/o sus productos metablicos BIOCONSERVANTE Efectos beneficiosos para la saludNuevas tcnicas
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El objetivo general del presente trabajo fue evaluar la posible utilizacin de
diversas sustancias (E. faecium y/o sus productos metablicos y la nisina)
como posibles bioconservantes y/o probiticos en filetes de dorada. Objetivo.
OBJETIVO
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Plan de trabajo.
FASE 1FASE 2FASE 3
Evolucin de la microbiota en filetes de dorada.Anlisis del efecto de la adicin de sustancias bioconservantes sobre microbiota de dorada.Anlisis del efecto de la adicin de sustancias bioconservantes sobre los parmetros fsico-qumicos en los filetes de dorada.PLAN DE TRABAJO
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Materiales y mtodos.
FASE
1Evolucin de la microbiota en filetes de dorada.
Almacenamiento 20 dasAtmsfera normal y en refrigeracinAnlisis microbiolgicos:
Aerbios mesfilosPsicrfilosEnterobacteriasBacterias lcticasBacillus cereusStaphylococcus aureusEnterococcusTOMA DE MUESTRA (das)0, 2, 4, 6, 8, 10, 12, 17, 20 400 gr. aprox.
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Materiales y mtodos.
FASE
2Anlisis del efecto de la adicin de sustancias bioconservantes sobre microbiota de dorada.
PulverizacinImpregnacin a vacoMTODOS DE APLICACIN
FASE
2Impregnacin a vaco
Fundamento: Sustitucin de los gases contenidos en el alimento por disoluciones de impregnacin.ABCDE25gr de muestra1 mL por lado del filete = 2 mL de solucin por muestraProduccin de vaco 0.9 bares,muestra en contacto con la solucin (10min.)Rotura de vaco muestra en contacto con solucin (5 min.)Escurrido 5 min.
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Materiales y mtodos.
FASE
2Anlisis del efecto de la adicin de sustancias bioconservantes sobre microbiota de dorada.
PulverizacinImpregnacin a vacoBIOCONSERVANTESMTODOS DE APLICACINC.CS.P.PNISINAC.CS.P.PNISINAC.CC.CS.P.PS.P.PAlmacenamiento:
Atmsfera normal
Refrigeracin
Anlisis microbiolgicos:
Aerbios mesfilosPsicrfilosEnterobacteriasBacterias lcticasBacillus cereusStaphylococcus aureusEnterococcusTOMA DE MUESTRA (das)0, 6, 8, 10, 12NISINANISINA nisina comercial (nisina from Streptococcus lactis, 2.5%) concentracin de 2000 IU/mg pH 5.3 Cultivo celular de E.faeciumSustrato parcialmente purificadoCultivo celular de E.faeciumSustrato parcialmente purificado
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Materiales y mtodos. Anlisis del efecto de la adicin de sustancias bioconservantes sobre los parmetros fsico-qumicos en los filetes de dorada.t=0 y t=8 ANLISIS FSICO-QUMICO
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Resultados y discusin.
EVOLUCIN DE LA MICROBIOTA EN FILETES DE DORADA.
FASE 1Aerobios MesfilosPsicrfilosEnterobacterias
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EVOLUCIN DE LA MICROBIOTA EN FILETES DE DORADA.
FASE 1 Resultados y discusin. = velocidad de crecimiento exponencial
= duracin de la fase de latencia
MPD: mxima densidad de poblacin
R2 = coeficiente de correlacin
GRUPOS MICROBIANOSMODELOMPDR2a) Aerobios mesfilosGompertz 0.7002.8338.6660.882Baranyi0.6903.2298.3520.897b) Aerobios psicrfilosGompertz 0.6413.6029.1460.960Baranyi0.5813.2058.8730.953c) EnterobacteriasGompertz 0.5489.3252.9270.731Baranyi0.4949.3082.9230.731
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Resultados y discusin. % De liquido retenido en la muestra despus de los tratamientos
Tcnica de aplicacinTratamientoIncremento depeso% InoculacinIMPREGNACIN A VACO SPP0,696 0,1762,536 0,567CC0,788 0,1882,875 0,774NISINA3,760 0,89112,593 2,853PULVERIZACINSPP0,516 0,0452,421 0,453CC0,451 0,3352,032 1,553NISINA0,951 0,6023,939 2,306
Tcnica de aplicacinTratamientoIncremento depeso% InoculacinIMPREGNACIN A VACO SPP0,696 0,1762,536 0,567CC0,788 0,1882,875 0,774NISINA3,760 0,89112,593 2,853PULVERIZACINSPP0,516 0,0452,421 0,453CC0,451 0,3352,032 1,553NISINA0,951 0,6023,939 2,306
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CULTIVO CELULAR E. faecium (CC) Resultados y discusin.
Grfico5
3.46494231937.16240325317.57178267990.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
5.25400049767.46440902677.45952250210.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
6.55704370497.49547050087.48098726870.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
8.59325317539.35926920128.10239292010.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
8.95338169749.40387572258.43910484071.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Mesfilos
mesofilos spp
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PSPP2.380.184
6PSPP4.940.059
8PSPP5.340.150
10PSPP9.280.121
12PSPP9.420.044
0ISPP4.000.215
6ISPP5.860.830
8ISPP5.960.182
10ISPP7.980.071
12ISPP8.470.958
mesofilos spp
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos cc
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos nisina
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PCC7.160.090
6PCC7.460.085
8PCC7.500.058
10PCC9.360.078
12PCC9.400.011
0ICC7.570.566
6ICC7.460.146
8ICC7.480.256
10ICC8.100.023
12ICC8.441.462
mesofilos nisina
0000.88336855290.88336855290.56575363170.56575363170.08958582850.0895858285
0000.84955834140.84955834140.14625514230.14625514230.08464248330.0846424833
0000.3711123180.3711123180.25560867520.25560867520.05818075620.0581807562
0000.5424751610.5424751610.0234538750.0234538750.0779237050.077923705
0001.01795575871.01795575871.46154439691.46154439690.01088194590.0108819459
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
mesfilos todos trat
0000.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
0000.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
0000.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
0000.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
0001.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
solo grficas
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PN1.211.404
6PN5.470.062
8PN6.030.238
10PN9.200.059
12PN9.390.107
0IN2.990.322
6IN4.910.043
8IN5.910.043
10IN6.120.079
12IN9.100.111
solo grficas
0000.88336855290.88336855290.32242743530.32242743531.40374396731.4037439673
0000.84955834140.84955834140.04303886430.04303886430.06230527630.0623052763
0000.3711123180.3711123180.04303886430.04303886430.23755027280.2375502728
0000.5424751610.5424751610.07913709130.07913709130.05889722840.0588972284
0001.01795575871.01795575870.11051926180.11051926180.10733193820.1073319382
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
0000.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
0000.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
0000.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
0000.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
0001.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.995051625
5.25400049764.94145437885.856884239
6.55704370495.33563034325.9581405915
8.59325317539.2820019127.976094699
8.95338169749.42444085688.4710579231
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.5717826799
5.25400049767.46440902677.4595225021
6.55704370497.49547050087.4809872687
8.59325317539.35926920128.1023929201
8.95338169749.40387572258.4391048407
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
3.46494231931.21342455292.9903134379
5.25400049765.47044020044.9108938368
6.55704370496.0269956515.9108938368
8.59325317539.20241012656.1216543831
8.95338169749.39196529359.0964873077
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.99031343790.88336855290.88336855290.18362880560.18362880560.21528272810.21528272810.08958582850.08958582850.56575363170.56575363171.40374396731.40374396730.32242743530.3224274353
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.91089383680.84955834140.84955834140.05940995940.05940995940.82999257270.82999257270.08464248330.08464248330.14625514230.14625514230.06230527630.06230527630.04303886430.0430388643
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.91089383680.3711123180.3711123180.14981080260.14981080260.18201724980.18201724980.05818075620.05818075620.25560867520.25560867520.23755027280.23755027280.04303886430.0430388643
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.12165438310.5424751610.5424751610.1206442920.1206442920.07077651150.07077651150.0779237050.0779237050.0234538750.0234538750.05889722840.05889722840.07913709130.0791370913
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.09648730771.01795575871.01795575870.04357725520.04357725520.95830670990.95830670990.01088194590.01088194591.46154439691.46154439690.10733193820.10733193820.11051926180.1105192618
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.57178267990.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
5.25400049767.46440902677.45952250210.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
6.55704370497.49547050087.48098726870.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
8.59325317539.35926920128.10239292010.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
8.95338169749.40387572258.43910484071.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231932.37793742783.9950516250.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
5.25400049764.94145437885.8568842390.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
6.55704370495.33563034325.95814059150.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
8.59325317539.2820019127.9760946990.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
8.95338169749.42444085688.47105792311.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231931.21342455292.99031343790.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
5.25400049765.47044020044.91089383680.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
6.55704370496.0269956515.91089383680.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
8.59325317539.20241012656.12165438310.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
8.95338169749.39196529359.09648730771.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Mesfilos
Grfico6
1.4668052197.08635134887.54673535471.40029381611.40029381610.02062822720.02062822720.56367030240.5636703024
3.33503001857.45935585017.25601994471.03284856721.03284856720.04210557010.04210557010.0592038960.059203896
3.89740052237.48067389256.84712996720.43574522870.43574522870.06959255380.06959255380.65224193020.6522419302
4.25103080287.58000913897.60702745940.39948070860.39948070860.12661132490.12661132490.05579472060.0557947206
4.93425061997.9690697017.17228281960.50155379540.50155379540.04453665050.04453665050.98265182450.9826518245
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Lab SPP
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PSPP0.000
6PSPP1.940.0764500935
8PSPP2.680.3285607366
10PSPP3.320.2033326716
12PSPP3.880.11623485
0ISPP3.410.3575480632
6ISPP5.150.3188595272
8ISPP5.320.4889302166
10ISPP5.770.1756702738
12ISPP4.580.3630604144
Lab SPP
000
000
000
000
000
Patrn
PSPP
ISPP
ufc
Lab
Lab CC
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PCC7.090.0206282272
6PCC7.460.0421055701
8PCC7.480.0695925538
10PCC7.580.1266113249
12PCC7.970.0445366505
0ICC7.550.5636703024
6ICC7.260.059203896
8ICC6.850.6522419302
10ICC7.610.0557947206
12ICC7.170.9826518245
Lab CC
000
000
000
000
000
Patrn
PCC
ICC
ufc
Lab
Lab nisina
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PN0.000
6PN0.000
8PN0.000
10PN2.570.0604689828
12PN3.230.7574973117
0IN0.000
6IN0.000
8IN0.000
10IN1.150.173799749
12IN0.000
Lab nisina
000
000
000
000
000
Patrn
PN
IN
ufc
Lab
grficas
grficas
1.46680521903.4131429269
3.33503001851.93704700685.1485152253
3.89740052232.68186384145.3231034333
4.25103080283.32221929475.7654308723
4.93425061993.88492580974.5818231269
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.5467353547
3.33503001857.45935585017.2560199447
3.89740052237.48067389256.8471299672
4.25103080287.58000913897.6070274594
4.93425061997.9690697017.1722828196
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
1.46680521900
3.335030018500
3.897400522300
4.25103080282.57088161511.1505149978
4.93425061993.22584760370
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
1.46680521903.41314292697.08635134887.546735354700000.35754806320.35754806320.02062822720.02062822720.56367030240.563670302400001.40029381611.4002938161
3.33503001851.93704700685.14851522537.45935585017.2560199447000.07645009350.07645009350.31885952720.31885952720.04210557010.04210557010.0592038960.05920389600001.03284856721.0328485672
3.89740052232.68186384145.32310343337.48067389256.8471299672000.32856073660.32856073660.48893021660.48893021660.06959255380.06959255380.65224193020.652241930200000.43574522870.4357452287
4.25103080283.32221929475.76543087237.58000913897.60702745942.57088161511.15051499780.20333267160.20333267160.17567027380.17567027380.12661132490.12661132490.05579472060.05579472060.06046898280.06046898280.1737997490.1737997490.39948070860.3994807086
4.93425061993.88492580974.58182312697.9690697017.17228281963.225847603700.116234850.116234850.36306041440.36306041440.04453665050.04453665050.98265182450.98265182450.75749731170.7574973117000.50155379540.5015537954
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.54673535471.40029381611.40029381610.02062822720.02062822720.56367030240.5636703024
3.33503001857.45935585017.25601994471.03284856721.03284856720.04210557010.04210557010.0592038960.059203896
3.89740052237.48067389256.84712996720.43574522870.43574522870.06959255380.06959255380.65224193020.6522419302
4.25103080287.58000913897.60702745940.39948070860.39948070860.12661132490.12661132490.05579472060.0557947206
4.93425061997.9690697017.17228281960.50155379540.50155379540.04453665050.04453665050.98265182450.9826518245
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.46680521903.41314292691.40029381611.4002938161000.35754806320.3575480632
3.33503001851.93704700685.14851522531.03284856721.03284856720.07645009350.07645009350.31885952720.3188595272
3.89740052232.68186384145.32310343330.43574522870.43574522870.32856073660.32856073660.48893021660.4889302166
4.25103080283.32221929475.76543087230.39948070860.39948070860.20333267160.20333267160.17567027380.1756702738
4.93425061993.88492580974.58182312690.50155379540.50155379540.116234850.116234850.36306041440.3630604144
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.466805219001.40029381611.400293816100
3.3350300185001.03284856721.032848567200
3.8974005223000.43574522870.435745228700
4.25103080282.57088161511.15051499780.39948070860.39948070860.1737997490.173799749
4.93425061993.225847603700.50155379540.501553795400
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Grfico6
0.13350499935.40837034585.63108734480.45230935910.45230935910.04725335850.04725335850.1268573280.126857328
06.41865972176.4051152183000.1024226760.1024226760.09596428130.0959642813
0.21683833266.20785545436.10125116630.49529365460.49529365460.05875443320.05875443320.06498581920.0649858192
06.07054536956.3136442694000.70447276590.70447276590.17105416160.1710541616
06.38541218146.6931748373000.13693763620.13693763620.14383519880.1438351988
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Enterococcus
Enterococos spp
tiempoMUESTRApromediodesvest
0Patrn0.1340.452309
6Patrn0.0000.000000
8Patrn0.2170.495294
10Patrn0.0000.000000
12Patrn0.0000.000000
0PSPP0.000
6PSPP0.000
8PSPP0.000
10PSPP0.000
12PSPP0.000
0ISPP0.000
6ISPP2.330.2282795407
8ISPP2.250.250701217
10ISPP3.890.601165854
12ISPP4.640.4329179762
Enterococos spp
000
000
000
000
000
Patrn
PSPP
ISPP
ufc
Enterococos
enterococos cc
tiempoMUESTRApromediodesvest
0Patrn0.1340.452309
6Patrn0.0000.000000
8Patrn0.2170.495294
10Patrn0.0000.000000
12Patrn0.0000.000000
0PCC5.410.0472533585
6PCC6.420.102422676
8PCC6.210.0587544332
10PCC6.070.7044727659
12PCC6.390.1369376362
0ICC5.630.126857328
6ICC6.410.0959642813
8ICC6.100.0649858192
10ICC6.310.1710541616
12ICC6.690.1438351988
enterococos cc
000
000
000
000
000
Patrn
PCC
ICC
ufc
Enterococos
enterococos nisina
tiempoMUESTRApromediodesvest
0Patrn0.1340.452309
6Patrn0.0000.000000
8Patrn0.2170.495294
10Patrn0.0000.000000
12Patrn0.0000.000000
0PN0.000
6PN0.000
8PN0.000
10PN0.000
12PN0.000
0IN0.330
6IN0.000
8IN0.000
10IN0.000
12IN0.000
enterococos nisina
000
000
000
000
000
Patrn
PN
IN
ufc
Enterococos
ENTEROCOCOS GRAF
ENTEROCOCOS GRAF
0.133504999300
002.3280443391
0.216838332602.2467958618
003.8887325067
004.6387325067
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
0.13350499935.40837034585.6310873448
06.41865972176.4051152183
0.21683833266.20785545436.1012511663
06.07054536956.3136442694
06.38541218146.6931748373
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
0.133504999300.3252574989
000
0.216838332600
000
000
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
0.1335049993005.40837034585.631087344800.32525749890000.04725335850.04725335850.1268573280.126857328000.45071430540.45071430540.45230935910.45230935910
002.32804433916.41865972176.40511521830000.22827954070.22827954070.1024226760.1024226760.09596428130.09596428130000000
0.216838332602.24679586186.20785545436.10125116630000.2507012170.2507012170.05875443320.05875443320.06498581920.064985819200000.49529365460.49529365460
003.88873250676.07054536956.31364426940000.6011658540.6011658540.70447276590.70447276590.17105416160.17105416160000000
004.63873250676.38541218146.69317483730000.43291797620.43291797620.13693763620.13693763620.14383519880.14383519880000000
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
0.13350499935.40837034585.63108734480.45230935910.45230935910.04725335850.04725335850.1268573280.126857328
06.41865972176.4051152183000.1024226760.1024226760.09596428130.0959642813
0.21683833266.20785545436.10125116630.49529365460.49529365460.05875443320.05875443320.06498581920.0649858192
06.07054536956.3136442694000.70447276590.70447276590.17105416160.1710541616
06.38541218146.6931748373000.13693763620.13693763620.14383519880.1438351988
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Enterococcus
3.46494231937.16240325317.57178267990.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
5.25400049767.46440902677.45952250210.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
6.55704370497.49547050087.48098726870.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
8.59325317539.35926920128.10239292010.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
8.95338169749.40387572258.43910484071.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Mesfilos
Grfico16
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
Sicro spp
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PSPP1.933098440.3302747428
6PSPP4.9043295510.2438072892
8PSPP5.47123656770.0303189548
10PSPP9.26861458230.1735496283
12PSPP9.53310032310.0328948372
0ISPP3.27167358050.3112649901
6ISPP5.85065773640.8159556746
8ISPP5.12134914431.0336690371
10ISPP7.94860053880.0676583421
12ISPP7.9854364991.3820126844
Sicro spp
000
000
000
000
000
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Sicro cc
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PCC6.98187981410.0213865046
6PCC7.40675621060.0350468208
8PCC7.46440902670.0846424833
10PCC9.35495535090.0422993598
12PCC8.5473922281.0915851714
0ICC7.90871434210.2623038684
6ICC6.96347142110.2099827182
8ICC6.83194263270.9657443155
10ICC8.06575462640.0391535197
12ICC8.48750802341.4667708399
Sicro cc
000
000
000
000
000
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Sicro nisina
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PN00
6PN5.21658636940.1298553182
8PN5.75614927030.3918945652
10PN9.34389637680.0239646473
12PN9.69491261250.2181215109
0IN1.76230450570.137070401
6IN4.47326693910.5035396045
8IN5.22326693910.420164501
10IN6.14520289140.0700066291
12IN8.958943170.1205351793
Sicro nisina
000
000
000
000
000
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
sicro todos trat
sicro todos trat
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.81314895591.933098443.27167358056.98187981417.908714342101.76230450571.01343680691.01343680690.33027474280.33027474280.31126499010.31126499010.02138650460.02138650460.26230386840.2623038684000.1370704010.137070401
5.24698656544.9043295515.85065773647.40675621066.96347142115.21658636944.47326693910.77918605210.77918605210.24380728920.24380728920.81595567460.81595567460.03504682080.03504682080.20998271820.20998271820.12985531820.12985531820.50353960450.5035396045
5.09150926625.47123656775.12134914437.46440902676.83194263275.75614927035.22326693910.72530393650.72530393650.03031895480.03031895481.03366903711.03366903710.08464248330.08464248330.96574431550.96574431550.39189456520.39189456520.4201645010.420164501
8.52508415489.26861458237.94860053889.35495535098.06575462649.34389637686.14520289140.37089042710.37089042710.17354962830.17354962830.06765834210.06765834210.04229935980.04229935980.03915351970.03915351970.02396464730.02396464730.07000662910.0700066291
8.87809046479.53310032317.9854364998.5473922288.48750802349.69491261258.958943171.20627037461.20627037460.03289483720.03289483721.38201268441.38201268441.09158517141.09158517141.46677083991.46677083990.21812151090.21812151090.12053517930.1205351793
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.2716735805
5.24698656544.9043295515.8506577364
5.09150926625.47123656775.1213491443
8.52508415489.26861458237.9486005388
8.87809046479.53310032317.985436499
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
2.81314895596.98187981417.9087143421
5.24698656547.40675621066.9634714211
5.09150926627.46440902676.8319426327
8.52508415489.35495535098.0657546264
8.87809046478.5473922288.4875080234
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
2.813148955901.7623045057
5.24698656545.21658636944.4732669391
5.09150926625.75614927035.2232669391
8.52508415489.34389637686.1452028914
8.87809046479.69491261258.95894317
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.813148955901.76230450571.01343680691.0134368069000.1370704010.137070401
5.24698656545.21658636944.47326693910.77918605210.77918605210.12985531820.12985531820.50353960450.5035396045
5.09150926625.75614927035.22326693910.72530393650.72530393650.39189456520.39189456520.4201645010.420164501
8.52508415489.34389637686.14520289140.37089042710.37089042710.02396464730.02396464730.07000662910.0700066291
8.87809046479.69491261258.958943171.20627037461.20627037460.21812151090.21812151090.12053517930.1205351793
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Psicrfilos
-
SUSTRATO PARCIALMENTE PURIFICADO (SPP) Resultados y discusin.
Grfico8
3.46494231932.37793742783.9950516250.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
5.25400049764.94145437885.8568842390.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
6.55704370495.33563034325.95814059150.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
8.59325317539.2820019127.9760946990.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
8.95338169749.42444085688.47105792311.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Mesfilos
mesofilos spp
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PSPP2.380.184
6PSPP4.940.059
8PSPP5.340.150
10PSPP9.280.121
12PSPP9.420.044
0ISPP4.000.215
6ISPP5.860.830
8ISPP5.960.182
10ISPP7.980.071
12ISPP8.470.958
mesofilos spp
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos cc
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos nisina
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PCC7.160.090
6PCC7.460.085
8PCC7.500.058
10PCC9.360.078
12PCC9.400.011
0ICC7.570.566
6ICC7.460.146
8ICC7.480.256
10ICC8.100.023
12ICC8.441.462
mesofilos nisina
0000.88336855290.88336855290.56575363170.56575363170.08958582850.0895858285
0000.84955834140.84955834140.14625514230.14625514230.08464248330.0846424833
0000.3711123180.3711123180.25560867520.25560867520.05818075620.0581807562
0000.5424751610.5424751610.0234538750.0234538750.0779237050.077923705
0001.01795575871.01795575871.46154439691.46154439690.01088194590.0108819459
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
mesfilos todos trat
0000.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
0000.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
0000.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
0000.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
0001.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
solo grficas
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PN1.211.404
6PN5.470.062
8PN6.030.238
10PN9.200.059
12PN9.390.107
0IN2.990.322
6IN4.910.043
8IN5.910.043
10IN6.120.079
12IN9.100.111
solo grficas
0000.88336855290.88336855290.32242743530.32242743531.40374396731.4037439673
0000.84955834140.84955834140.04303886430.04303886430.06230527630.0623052763
0000.3711123180.3711123180.04303886430.04303886430.23755027280.2375502728
0000.5424751610.5424751610.07913709130.07913709130.05889722840.0588972284
0001.01795575871.01795575870.11051926180.11051926180.10733193820.1073319382
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
0000.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
0000.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
0000.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
0000.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
0001.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.995051625
5.25400049764.94145437885.856884239
6.55704370495.33563034325.9581405915
8.59325317539.2820019127.976094699
8.95338169749.42444085688.4710579231
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.5717826799
5.25400049767.46440902677.4595225021
6.55704370497.49547050087.4809872687
8.59325317539.35926920128.1023929201
8.95338169749.40387572258.4391048407
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
3.46494231931.21342455292.9903134379
5.25400049765.47044020044.9108938368
6.55704370496.0269956515.9108938368
8.59325317539.20241012656.1216543831
8.95338169749.39196529359.0964873077
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.99031343790.88336855290.88336855290.18362880560.18362880560.21528272810.21528272810.08958582850.08958582850.56575363170.56575363171.40374396731.40374396730.32242743530.3224274353
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.91089383680.84955834140.84955834140.05940995940.05940995940.82999257270.82999257270.08464248330.08464248330.14625514230.14625514230.06230527630.06230527630.04303886430.0430388643
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.91089383680.3711123180.3711123180.14981080260.14981080260.18201724980.18201724980.05818075620.05818075620.25560867520.25560867520.23755027280.23755027280.04303886430.0430388643
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.12165438310.5424751610.5424751610.1206442920.1206442920.07077651150.07077651150.0779237050.0779237050.0234538750.0234538750.05889722840.05889722840.07913709130.0791370913
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.09648730771.01795575871.01795575870.04357725520.04357725520.95830670990.95830670990.01088194590.01088194591.46154439691.46154439690.10733193820.10733193820.11051926180.1105192618
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.57178267990.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
5.25400049767.46440902677.45952250210.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
6.55704370497.49547050087.48098726870.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
8.59325317539.35926920128.10239292010.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
8.95338169749.40387572258.43910484071.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231932.37793742783.9950516250.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
5.25400049764.94145437885.8568842390.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
6.55704370495.33563034325.95814059150.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
8.59325317539.2820019127.9760946990.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
8.95338169749.42444085688.47105792311.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231931.21342455292.99031343790.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
5.25400049765.47044020044.91089383680.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
6.55704370496.0269956515.91089383680.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
8.59325317539.20241012656.12165438310.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
8.95338169749.39196529359.09648730771.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Mesfilos
Grfico19
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
Sicro spp
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PSPP1.933098440.3302747428
6PSPP4.9043295510.2438072892
8PSPP5.47123656770.0303189548
10PSPP9.26861458230.1735496283
12PSPP9.53310032310.0328948372
0ISPP3.27167358050.3112649901
6ISPP5.85065773640.8159556746
8ISPP5.12134914431.0336690371
10ISPP7.94860053880.0676583421
12ISPP7.9854364991.3820126844
Sicro spp
000
000
000
000
000
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Sicro cc
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PCC6.98187981410.0213865046
6PCC7.40675621060.0350468208
8PCC7.46440902670.0846424833
10PCC9.35495535090.0422993598
12PCC8.5473922281.0915851714
0ICC7.90871434210.2623038684
6ICC6.96347142110.2099827182
8ICC6.83194263270.9657443155
10ICC8.06575462640.0391535197
12ICC8.48750802341.4667708399
Sicro cc
000
000
000
000
000
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Sicro nisina
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PN00
6PN5.21658636940.1298553182
8PN5.75614927030.3918945652
10PN9.34389637680.0239646473
12PN9.69491261250.2181215109
0IN1.76230450570.137070401
6IN4.47326693910.5035396045
8IN5.22326693910.420164501
10IN6.14520289140.0700066291
12IN8.958943170.1205351793
Sicro nisina
000
000
000
000
000
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
sicro todos trat
sicro todos trat
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.81314895591.933098443.27167358056.98187981417.908714342101.76230450571.01343680691.01343680690.33027474280.33027474280.31126499010.31126499010.02138650460.02138650460.26230386840.2623038684000.1370704010.137070401
5.24698656544.9043295515.85065773647.40675621066.96347142115.21658636944.47326693910.77918605210.77918605210.24380728920.24380728920.81595567460.81595567460.03504682080.03504682080.20998271820.20998271820.12985531820.12985531820.50353960450.5035396045
5.09150926625.47123656775.12134914437.46440902676.83194263275.75614927035.22326693910.72530393650.72530393650.03031895480.03031895481.03366903711.03366903710.08464248330.08464248330.96574431550.96574431550.39189456520.39189456520.4201645010.420164501
8.52508415489.26861458237.94860053889.35495535098.06575462649.34389637686.14520289140.37089042710.37089042710.17354962830.17354962830.06765834210.06765834210.04229935980.04229935980.03915351970.03915351970.02396464730.02396464730.07000662910.0700066291
8.87809046479.53310032317.9854364998.5473922288.48750802349.69491261258.958943171.20627037461.20627037460.03289483720.03289483721.38201268441.38201268441.09158517141.09158517141.46677083991.46677083990.21812151090.21812151090.12053517930.1205351793
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.2716735805
5.24698656544.9043295515.8506577364
5.09150926625.47123656775.1213491443
8.52508415489.26861458237.9486005388
8.87809046479.53310032317.985436499
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
2.81314895596.98187981417.9087143421
5.24698656547.40675621066.9634714211
5.09150926627.46440902676.8319426327
8.52508415489.35495535098.0657546264
8.87809046478.5473922288.4875080234
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
2.813148955901.7623045057
5.24698656545.21658636944.4732669391
5.09150926625.75614927035.2232669391
8.52508415489.34389637686.1452028914
8.87809046479.69491261258.95894317
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.813148955901.76230450571.01343680691.0134368069000.1370704010.137070401
5.24698656545.21658636944.47326693910.77918605210.77918605210.12985531820.12985531820.50353960450.5035396045
5.09150926625.75614927035.22326693910.72530393650.72530393650.39189456520.39189456520.4201645010.420164501
8.52508415489.34389637686.14520289140.37089042710.37089042710.02396464730.02396464730.07000662910.0700066291
8.87809046479.69491261258.958943171.20627037461.20627037460.21812151090.21812151090.12053517930.1205351793
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Psicrfilos
Grfico18
1.46680521903.41314292691.40029381611.4002938161000.35754806320.3575480632
3.33503001851.93704700685.14851522531.03284856721.03284856720.07645009350.07645009350.31885952720.3188595272
3.89740052232.68186384145.32310343330.43574522870.43574522870.32856073660.32856073660.48893021660.4889302166
4.25103080283.32221929475.76543087230.39948070860.39948070860.20333267160.20333267160.17567027380.1756702738
4.93425061993.88492580974.58182312690.50155379540.50155379540.116234850.116234850.36306041440.3630604144
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Lab SPP
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PSPP0.000
6PSPP1.940.0764500935
8PSPP2.680.3285607366
10PSPP3.320.2033326716
12PSPP3.880.11623485
0ISPP3.410.3575480632
6ISPP5.150.3188595272
8ISPP5.320.4889302166
10ISPP5.770.1756702738
12ISPP4.580.3630604144
Lab SPP
000
000
000
000
000
Patrn
PSPP
ISPP
ufc
Lab
Lab CC
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PCC7.090.0206282272
6PCC7.460.0421055701
8PCC7.480.0695925538
10PCC7.580.1266113249
12PCC7.970.0445366505
0ICC7.550.5636703024
6ICC7.260.059203896
8ICC6.850.6522419302
10ICC7.610.0557947206
12ICC7.170.9826518245
Lab CC
000
000
000
000
000
Patrn
PCC
ICC
ufc
Lab
Lab nisina
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PN0.000
6PN0.000
8PN0.000
10PN2.570.0604689828
12PN3.230.7574973117
0IN0.000
6IN0.000
8IN0.000
10IN1.150.173799749
12IN0.000
Lab nisina
000
000
000
000
000
Patrn
PN
IN
ufc
Lab
grficas
grficas
1.46680521903.4131429269
3.33503001851.93704700685.1485152253
3.89740052232.68186384145.3231034333
4.25103080283.32221929475.7654308723
4.93425061993.88492580974.5818231269
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.5467353547
3.33503001857.45935585017.2560199447
3.89740052237.48067389256.8471299672
4.25103080287.58000913897.6070274594
4.93425061997.9690697017.1722828196
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
1.46680521900
3.335030018500
3.897400522300
4.25103080282.57088161511.1505149978
4.93425061993.22584760370
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
1.46680521903.41314292697.08635134887.546735354700000.35754806320.35754806320.02062822720.02062822720.56367030240.563670302400001.40029381611.4002938161
3.33503001851.93704700685.14851522537.45935585017.2560199447000.07645009350.07645009350.31885952720.31885952720.04210557010.04210557010.0592038960.05920389600001.03284856721.0328485672
3.89740052232.68186384145.32310343337.48067389256.8471299672000.32856073660.32856073660.48893021660.48893021660.06959255380.06959255380.65224193020.652241930200000.43574522870.4357452287
4.25103080283.32221929475.76543087237.58000913897.60702745942.57088161511.15051499780.20333267160.20333267160.17567027380.17567027380.12661132490.12661132490.05579472060.05579472060.06046898280.06046898280.1737997490.1737997490.39948070860.3994807086
4.93425061993.88492580974.58182312697.9690697017.17228281963.225847603700.116234850.116234850.36306041440.36306041440.04453665050.04453665050.98265182450.98265182450.75749731170.7574973117000.50155379540.5015537954
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.54673535471.40029381611.40029381610.02062822720.02062822720.56367030240.5636703024
3.33503001857.45935585017.25601994471.03284856721.03284856720.04210557010.04210557010.0592038960.059203896
3.89740052237.48067389256.84712996720.43574522870.43574522870.06959255380.06959255380.65224193020.6522419302
4.25103080287.58000913897.60702745940.39948070860.39948070860.12661132490.12661132490.05579472060.0557947206
4.93425061997.9690697017.17228281960.50155379540.50155379540.04453665050.04453665050.98265182450.9826518245
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.46680521903.41314292691.40029381611.4002938161000.35754806320.3575480632
3.33503001851.93704700685.14851522531.03284856721.03284856720.07645009350.07645009350.31885952720.3188595272
3.89740052232.68186384145.32310343330.43574522870.43574522870.32856073660.32856073660.48893021660.4889302166
4.25103080283.32221929475.76543087230.39948070860.39948070860.20333267160.20333267160.17567027380.1756702738
4.93425061993.88492580974.58182312690.50155379540.50155379540.116234850.116234850.36306041440.3630604144
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.466805219001.40029381611.400293816100
3.3350300185001.03284856721.032848567200
3.8974005223000.43574522870.435745228700
4.25103080282.57088161511.15051499780.39948070860.39948070860.1737997490.173799749
4.93425061993.225847603700.50155379540.501553795400
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Grfico4
002.6424938734000.12032537020.1203253702
004.1717483916000.18448263070.1844826307
0.563368540303.76451839670.82349562730.82349562730.54608848880.5460884888
2.342926792404.13719439780.68188761010.68188761010.17048144820.1704814482
2.341461959404.40645667830.90956263930.90956263930.00910470280.0091047028
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
S.aureus
Hoja1
Hoja1
002.6424938734000.12032537020.1203253702
004.1717483916000.18448263070.1844826307
0.563368540303.76451839670.82349562730.82349562730.54608848880.5460884888
2.342926792404.13719439780.68188761010.68188761010.17048144820.1704814482
2.341461959404.40645667830.90956263930.90956263930.00910470280.0091047028
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
S.aureus
Hoja2
Hoja3
MBD000607D8.xls
Grfico16
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
Sicro spp
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PSPP1.933098440.3302747428
6PSPP4.9043295510.2438072892
8PSPP5.47123656770.0303189548
10PSPP9.26861458230.1735496283
12PSPP9.53310032310.0328948372
0ISPP3.27167358050.3112649901
6ISPP5.85065773640.8159556746
8ISPP5.12134914431.0336690371
10ISPP7.94860053880.0676583421
12ISPP7.9854364991.3820126844
Sicro spp
000
000
000
000
000
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Sicro cc
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PCC6.98187981410.0213865046
6PCC7.40675621060.0350468208
8PCC7.46440902670.0846424833
10PCC9.35495535090.0422993598
12PCC8.5473922281.0915851714
0ICC7.90871434210.2623038684
6ICC6.96347142110.2099827182
8ICC6.83194263270.9657443155
10ICC8.06575462640.0391535197
12ICC8.48750802341.4667708399
Sicro cc
000
000
000
000
000
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Sicro nisina
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PN00
6PN5.21658636940.1298553182
8PN5.75614927030.3918945652
10PN9.34389637680.0239646473
12PN9.69491261250.2181215109
0IN1.76230450570.137070401
6IN4.47326693910.5035396045
8IN5.22326693910.420164501
10IN6.14520289140.0700066291
12IN8.958943170.1205351793
Sicro nisina
000
000
000
000
000
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
sicro todos trat
sicro todos trat
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.81314895591.933098443.27167358056.98187981417.908714342101.76230450571.01343680691.01343680690.33027474280.33027474280.31126499010.31126499010.02138650460.02138650460.26230386840.2623038684000.1370704010.137070401
5.24698656544.9043295515.85065773647.40675621066.96347142115.21658636944.47326693910.77918605210.77918605210.24380728920.24380728920.81595567460.81595567460.03504682080.03504682080.20998271820.20998271820.12985531820.12985531820.50353960450.5035396045
5.09150926625.47123656775.12134914437.46440902676.83194263275.75614927035.22326693910.72530393650.72530393650.03031895480.03031895481.03366903711.03366903710.08464248330.08464248330.96574431550.96574431550.39189456520.39189456520.4201645010.420164501
8.52508415489.26861458237.94860053889.35495535098.06575462649.34389637686.14520289140.37089042710.37089042710.17354962830.17354962830.06765834210.06765834210.04229935980.04229935980.03915351970.03915351970.02396464730.02396464730.07000662910.0700066291
8.87809046479.53310032317.9854364998.5473922288.48750802349.69491261258.958943171.20627037461.20627037460.03289483720.03289483721.38201268441.38201268441.09158517141.09158517141.46677083991.46677083990.21812151090.21812151090.12053517930.1205351793
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.2716735805
5.24698656544.9043295515.8506577364
5.09150926625.47123656775.1213491443
8.52508415489.26861458237.9486005388
8.87809046479.53310032317.985436499
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
2.81314895596.98187981417.9087143421
5.24698656547.40675621066.9634714211
5.09150926627.46440902676.8319426327
8.52508415489.35495535098.0657546264
8.87809046478.5473922288.4875080234
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
2.813148955901.7623045057
5.24698656545.21658636944.4732669391
5.09150926625.75614927035.2232669391
8.52508415489.34389637686.1452028914
8.87809046479.69491261258.95894317
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.813148955901.76230450571.01343680691.0134368069000.1370704010.137070401
5.24698656545.21658636944.47326693910.77918605210.77918605210.12985531820.12985531820.50353960450.5035396045
5.09150926625.75614927035.22326693910.72530393650.72530393650.39189456520.39189456520.4201645010.420164501
8.52508415489.34389637686.14520289140.37089042710.37089042710.02396464730.02396464730.07000662910.0700066291
8.87809046479.69491261258.958943171.20627037461.20627037460.21812151090.21812151090.12053517930.1205351793
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Psicrfilos
MBD0007C33C.xls
Grfico19
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
Sicro spp
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PSPP1.933098440.3302747428
6PSPP4.9043295510.2438072892
8PSPP5.47123656770.0303189548
10PSPP9.26861458230.1735496283
12PSPP9.53310032310.0328948372
0ISPP3.27167358050.3112649901
6ISPP5.85065773640.8159556746
8ISPP5.12134914431.0336690371
10ISPP7.94860053880.0676583421
12ISPP7.9854364991.3820126844
Sicro spp
000
000
000
000
000
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Sicro cc
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PCC6.98187981410.0213865046
6PCC7.40675621060.0350468208
8PCC7.46440902670.0846424833
10PCC9.35495535090.0422993598
12PCC8.5473922281.0915851714
0ICC7.90871434210.2623038684
6ICC6.96347142110.2099827182
8ICC6.83194263270.9657443155
10ICC8.06575462640.0391535197
12ICC8.48750802341.4667708399
Sicro cc
000
000
000
000
000
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Sicro nisina
tiempoMUESTRApromediodesvest
0Patrn2.81314895591.0134368069
6Patrn5.24698656540.7791860521
8Patrn5.09150926620.7253039365
10Patrn8.52508415480.3708904271
12Patrn8.87809046471.2062703746
0PN00
6PN5.21658636940.1298553182
8PN5.75614927030.3918945652
10PN9.34389637680.0239646473
12PN9.69491261250.2181215109
0IN1.76230450570.137070401
6IN4.47326693910.5035396045
8IN5.22326693910.420164501
10IN6.14520289140.0700066291
12IN8.958943170.1205351793
Sicro nisina
000
000
000
000
000
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
sicro todos trat
sicro todos trat
2.81314895596.98187981417.90871434211.01343680691.01343680690.02138650460.02138650460.26230386840.2623038684
5.24698656547.40675621066.96347142110.77918605210.77918605210.03504682080.03504682080.20998271820.2099827182
5.09150926627.46440902676.83194263270.72530393650.72530393650.08464248330.08464248330.96574431550.9657443155
8.52508415489.35495535098.06575462640.37089042710.37089042710.04229935980.04229935980.03915351970.0391535197
8.87809046478.5473922288.48750802341.20627037461.20627037461.09158517141.09158517141.46677083991.4667708399
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.81314895591.933098443.27167358056.98187981417.908714342101.76230450571.01343680691.01343680690.33027474280.33027474280.31126499010.31126499010.02138650460.02138650460.26230386840.2623038684000.1370704010.137070401
5.24698656544.9043295515.85065773647.40675621066.96347142115.21658636944.47326693910.77918605210.77918605210.24380728920.24380728920.81595567460.81595567460.03504682080.03504682080.20998271820.20998271820.12985531820.12985531820.50353960450.5035396045
5.09150926625.47123656775.12134914437.46440902676.83194263275.75614927035.22326693910.72530393650.72530393650.03031895480.03031895481.03366903711.03366903710.08464248330.08464248330.96574431550.96574431550.39189456520.39189456520.4201645010.420164501
8.52508415489.26861458237.94860053889.35495535098.06575462649.34389637686.14520289140.37089042710.37089042710.17354962830.17354962830.06765834210.06765834210.04229935980.04229935980.03915351970.03915351970.02396464730.02396464730.07000662910.0700066291
8.87809046479.53310032317.9854364998.5473922288.48750802349.69491261258.958943171.20627037461.20627037460.03289483720.03289483721.38201268441.38201268441.09158517141.09158517141.46677083991.46677083990.21812151090.21812151090.12053517930.1205351793
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.2716735805
5.24698656544.9043295515.8506577364
5.09150926625.47123656775.1213491443
8.52508415489.26861458237.9486005388
8.87809046479.53310032317.985436499
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
2.81314895596.98187981417.9087143421
5.24698656547.40675621066.9634714211
5.09150926627.46440902676.8319426327
8.52508415489.35495535098.0657546264
8.87809046478.5473922288.4875080234
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
2.813148955901.7623045057
5.24698656545.21658636944.4732669391
5.09150926625.75614927035.2232669391
8.52508415489.34389637686.1452028914
8.87809046479.69491261258.95894317
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
2.81314895591.933098443.27167358051.01343680691.01343680690.33027474280.33027474280.31126499010.3112649901
5.24698656544.9043295515.85065773640.77918605210.77918605210.24380728920.24380728920.81595567460.8159556746
5.09150926625.47123656775.12134914430.72530393650.72530393650.03031895480.03031895481.03366903711.0336690371
8.52508415489.26861458237.94860053880.37089042710.37089042710.17354962830.17354962830.06765834210.0676583421
8.87809046479.53310032317.9854364991.20627037461.20627037460.03289483720.03289483721.38201268441.3820126844
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Psicrfilos
2.813148955901.76230450571.01343680691.0134368069000.1370704010.137070401
5.24698656545.21658636944.47326693910.77918605210.77918605210.12985531820.12985531820.50353960450.5035396045
5.09150926625.75614927035.22326693910.72530393650.72530393650.39189456520.39189456520.4201645010.420164501
8.52508415489.34389637686.14520289140.37089042710.37089042710.02396464730.02396464730.07000662910.0700066291
8.87809046479.69491261258.958943171.20627037461.20627037460.21812151090.21812151090.12053517930.1205351793
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Psicrfilos
MBD000A7757.xls
Grfico18
1.46680521903.41314292691.40029381611.4002938161000.35754806320.3575480632
3.33503001851.93704700685.14851522531.03284856721.03284856720.07645009350.07645009350.31885952720.3188595272
3.89740052232.68186384145.32310343330.43574522870.43574522870.32856073660.32856073660.48893021660.4889302166
4.25103080283.32221929475.76543087230.39948070860.39948070860.20333267160.20333267160.17567027380.1756702738
4.93425061993.88492580974.58182312690.50155379540.50155379540.116234850.116234850.36306041440.3630604144
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Lab SPP
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PSPP0.000
6PSPP1.940.0764500935
8PSPP2.680.3285607366
10PSPP3.320.2033326716
12PSPP3.880.11623485
0ISPP3.410.3575480632
6ISPP5.150.3188595272
8ISPP5.320.4889302166
10ISPP5.770.1756702738
12ISPP4.580.3630604144
Lab SPP
000
000
000
000
000
Patrn
PSPP
ISPP
ufc
Lab
Lab CC
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PCC7.090.0206282272
6PCC7.460.0421055701
8PCC7.480.0695925538
10PCC7.580.1266113249
12PCC7.970.0445366505
0ICC7.550.5636703024
6ICC7.260.059203896
8ICC6.850.6522419302
10ICC7.610.0557947206
12ICC7.170.9826518245
Lab CC
000
000
000
000
000
Patrn
PCC
ICC
ufc
Lab
Lab nisina
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PN0.000
6PN0.000
8PN0.000
10PN2.570.0604689828
12PN3.230.7574973117
0IN0.000
6IN0.000
8IN0.000
10IN1.150.173799749
12IN0.000
Lab nisina
000
000
000
000
000
Patrn
PN
IN
ufc
Lab
grficas
grficas
1.46680521903.4131429269
3.33503001851.93704700685.1485152253
3.89740052232.68186384145.3231034333
4.25103080283.32221929475.7654308723
4.93425061993.88492580974.5818231269
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.5467353547
3.33503001857.45935585017.2560199447
3.89740052237.48067389256.8471299672
4.25103080287.58000913897.6070274594
4.93425061997.9690697017.1722828196
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
1.46680521900
3.335030018500
3.897400522300
4.25103080282.57088161511.1505149978
4.93425061993.22584760370
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
1.46680521903.41314292697.08635134887.546735354700000.35754806320.35754806320.02062822720.02062822720.56367030240.563670302400001.40029381611.4002938161
3.33503001851.93704700685.14851522537.45935585017.2560199447000.07645009350.07645009350.31885952720.31885952720.04210557010.04210557010.0592038960.05920389600001.03284856721.0328485672
3.89740052232.68186384145.32310343337.48067389256.8471299672000.32856073660.32856073660.48893021660.48893021660.06959255380.06959255380.65224193020.652241930200000.43574522870.4357452287
4.25103080283.32221929475.76543087237.58000913897.60702745942.57088161511.15051499780.20333267160.20333267160.17567027380.17567027380.12661132490.12661132490.05579472060.05579472060.06046898280.06046898280.1737997490.1737997490.39948070860.3994807086
4.93425061993.88492580974.58182312697.9690697017.17228281963.225847603700.116234850.116234850.36306041440.36306041440.04453665050.04453665050.98265182450.98265182450.75749731170.7574973117000.50155379540.5015537954
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.54673535471.40029381611.40029381610.02062822720.02062822720.56367030240.5636703024
3.33503001857.45935585017.25601994471.03284856721.03284856720.04210557010.04210557010.0592038960.059203896
3.89740052237.48067389256.84712996720.43574522870.43574522870.06959255380.06959255380.65224193020.6522419302
4.25103080287.58000913897.60702745940.39948070860.39948070860.12661132490.12661132490.05579472060.0557947206
4.93425061997.9690697017.17228281960.50155379540.50155379540.04453665050.04453665050.98265182450.9826518245
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.46680521903.41314292691.40029381611.4002938161000.35754806320.3575480632
3.33503001851.93704700685.14851522531.03284856721.03284856720.07645009350.07645009350.31885952720.3188595272
3.89740052232.68186384145.32310343330.43574522870.43574522870.32856073660.32856073660.48893021660.4889302166
4.25103080283.32221929475.76543087230.39948070860.39948070860.20333267160.20333267160.17567027380.1756702738
4.93425061993.88492580974.58182312690.50155379540.50155379540.116234850.116234850.36306041440.3630604144
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
1.466805219001.40029381611.400293816100
3.3350300185001.03284856721.032848567200
3.8974005223000.43574522870.435745228700
4.25103080282.57088161511.15051499780.39948070860.39948070860.1737997490.173799749
4.93425061993.225847603700.50155379540.501553795400
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
MBD0007C091.xls
Grfico8
3.46494231932.37793742783.9950516250.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
5.25400049764.94145437885.8568842390.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
6.55704370495.33563034325.95814059150.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
8.59325317539.2820019127.9760946990.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
8.95338169749.42444085688.47105792311.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Mesfilos
mesofilos spp
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PSPP2.380.184
6PSPP4.940.059
8PSPP5.340.150
10PSPP9.280.121
12PSPP9.420.044
0ISPP4.000.215
6ISPP5.860.830
8ISPP5.960.182
10ISPP7.980.071
12ISPP8.470.958
mesofilos spp
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos cc
0000.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
0000.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
0000.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
0000.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
0001.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
mesofilos nisina
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PCC7.160.090
6PCC7.460.085
8PCC7.500.058
10PCC9.360.078
12PCC9.400.011
0ICC7.570.566
6ICC7.460.146
8ICC7.480.256
10ICC8.100.023
12ICC8.441.462
mesofilos nisina
0000.88336855290.88336855290.56575363170.56575363170.08958582850.0895858285
0000.84955834140.84955834140.14625514230.14625514230.08464248330.0846424833
0000.3711123180.3711123180.25560867520.25560867520.05818075620.0581807562
0000.5424751610.5424751610.0234538750.0234538750.0779237050.077923705
0001.01795575871.01795575871.46154439691.46154439690.01088194590.0108819459
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
mesfilos todos trat
0000.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
0000.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
0000.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
0000.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
0001.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
solo grficas
tiempoMUESTRApromediodesvest
0Patrn3.460.883
6Patrn5.250.850
8Patrn6.560.371
10Patrn8.590.542
12Patrn8.951.018
0PN1.211.404
6PN5.470.062
8PN6.030.238
10PN9.200.059
12PN9.390.107
0IN2.990.322
6IN4.910.043
8IN5.910.043
10IN6.120.079
12IN9.100.111
solo grficas
0000.88336855290.88336855290.32242743530.32242743531.40374396731.4037439673
0000.84955834140.84955834140.04303886430.04303886430.06230527630.0623052763
0000.3711123180.3711123180.04303886430.04303886430.23755027280.2375502728
0000.5424751610.5424751610.07913709130.07913709130.05889722840.0588972284
0001.01795575871.01795575870.11051926180.11051926180.10733193820.1073319382
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
0000.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
0000.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
0000.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
0000.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
0001.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.9903134379
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.9108938368
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.9108938368
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.1216543831
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.0964873077
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.995051625
5.25400049764.94145437885.856884239
6.55704370495.33563034325.9581405915
8.59325317539.2820019127.976094699
8.95338169749.42444085688.4710579231
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.5717826799
5.25400049767.46440902677.4595225021
6.55704370497.49547050087.4809872687
8.59325317539.35926920128.1023929201
8.95338169749.40387572258.4391048407
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
3.46494231931.21342455292.9903134379
5.25400049765.47044020044.9108938368
6.55704370496.0269956515.9108938368
8.59325317539.20241012656.1216543831
8.95338169749.39196529359.0964873077
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231932.37793742783.9950516257.16240325317.57178267991.21342455292.99031343790.88336855290.88336855290.18362880560.18362880560.21528272810.21528272810.08958582850.08958582850.56575363170.56575363171.40374396731.40374396730.32242743530.3224274353
5.25400049764.94145437885.8568842397.46440902677.45952250215.47044020044.91089383680.84955834140.84955834140.05940995940.05940995940.82999257270.82999257270.08464248330.08464248330.14625514230.14625514230.06230527630.06230527630.04303886430.0430388643
6.55704370495.33563034325.95814059157.49547050087.48098726876.0269956515.91089383680.3711123180.3711123180.14981080260.14981080260.18201724980.18201724980.05818075620.05818075620.25560867520.25560867520.23755027280.23755027280.04303886430.0430388643
8.59325317539.2820019127.9760946999.35926920128.10239292019.20241012656.12165438310.5424751610.5424751610.1206442920.1206442920.07077651150.07077651150.0779237050.0779237050.0234538750.0234538750.05889722840.05889722840.07913709130.0791370913
8.95338169749.42444085688.47105792319.40387572258.43910484079.39196529359.09648730771.01795575871.01795575870.04357725520.04357725520.95830670990.95830670990.01088194590.01088194591.46154439691.46154439690.10733193820.10733193820.11051926180.1105192618
Patrn
PSPP
ISPP
PCC
ICC
PN
IN
Tiempo (das)
Log (ufc/g)
3.46494231937.16240325317.57178267990.88336855290.88336855290.08958582850.08958582850.56575363170.5657536317
5.25400049767.46440902677.45952250210.84955834140.84955834140.08464248330.08464248330.14625514230.1462551423
6.55704370497.49547050087.48098726870.3711123180.3711123180.05818075620.05818075620.25560867520.2556086752
8.59325317539.35926920128.10239292010.5424751610.5424751610.0779237050.0779237050.0234538750.023453875
8.95338169749.40387572258.43910484071.01795575871.01795575870.01088194590.01088194591.46154439691.4615443969
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231932.37793742783.9950516250.88336855290.88336855290.18362880560.18362880560.21528272810.2152827281
5.25400049764.94145437885.8568842390.84955834140.84955834140.05940995940.05940995940.82999257270.8299925727
6.55704370495.33563034325.95814059150.3711123180.3711123180.14981080260.14981080260.18201724980.1820172498
8.59325317539.2820019127.9760946990.5424751610.5424751610.1206442920.1206442920.07077651150.0707765115
8.95338169749.42444085688.47105792311.01795575871.01795575870.04357725520.04357725520.95830670990.9583067099
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
Mesfilos
3.46494231931.21342455292.99031343790.88336855290.88336855291.40374396731.40374396730.32242743530.3224274353
5.25400049765.47044020044.91089383680.84955834140.84955834140.06230527630.06230527630.04303886430.0430388643
6.55704370496.0269956515.91089383680.3711123180.3711123180.23755027280.23755027280.04303886430.0430388643
8.59325317539.20241012656.12165438310.5424751610.5424751610.05889722840.05889722840.07913709130.0791370913
8.95338169749.39196529359.09648730771.01795575871.01795575870.10733193820.10733193820.11051926180.1105192618
Patrn
PN
IN
Tiempo (das)
Log (ufc/g)
Mesfilos
MBD0005E350.xls
Grfico6
1.4668052197.08635134887.54673535471.40029381611.40029381610.02062822720.02062822720.56367030240.5636703024
3.33503001857.45935585017.25601994471.03284856721.03284856720.04210557010.04210557010.0592038960.059203896
3.89740052237.48067389256.84712996720.43574522870.43574522870.06959255380.06959255380.65224193020.6522419302
4.25103080287.58000913897.60702745940.39948070860.39948070860.12661132490.12661132490.05579472060.0557947206
4.93425061997.9690697017.17228281960.50155379540.50155379540.04453665050.04453665050.98265182450.9826518245
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
Bacterias cido lcticas
Lab SPP
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PSPP0.000
6PSPP1.940.0764500935
8PSPP2.680.3285607366
10PSPP3.320.2033326716
12PSPP3.880.11623485
0ISPP3.410.3575480632
6ISPP5.150.3188595272
8ISPP5.320.4889302166
10ISPP5.770.1756702738
12ISPP4.580.3630604144
Lab SPP
000
000
000
000
000
Patrn
PSPP
ISPP
ufc
Lab
Lab CC
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PCC7.090.0206282272
6PCC7.460.0421055701
8PCC7.480.0695925538
10PCC7.580.1266113249
12PCC7.970.0445366505
0ICC7.550.5636703024
6ICC7.260.059203896
8ICC6.850.6522419302
10ICC7.610.0557947206
12ICC7.170.9826518245
Lab CC
000
000
000
000
000
Patrn
PCC
ICC
ufc
Lab
Lab nisina
tiempoMUESTRApromediodesvest
0Patrn1.4671.400294
6Patrn3.3351.032849
8Patrn3.8970.435745
10Patrn4.2510.399481
12Patrn4.9340.501554
0PN0.000
6PN0.000
8PN0.000
10PN2.570.0604689828
12PN3.230.7574973117
0IN0.000
6IN0.000
8IN0.000
10IN1.150.173799749
12IN0.000
Lab nisina
000
000
000
000
000
Patrn
PN
IN
ufc
Lab
grficas
grficas
1.46680521903.4131429269
3.33503001851.93704700685.1485152253
3.89740052232.68186384145.3231034333
4.25103080283.32221929475.7654308723
4.93425061993.88492580974.5818231269
Patrn
PSPP
ISPP
Tiempo (das)
Log (ufc/g)
1.4668052197.08635134887.5467353547
3.33503001857.45935585017.2560199447
3.89740052237.48067389256.8471299672
4.25103080287.58000913897.6070274594
4.93425061997.9690697017.1722828196
Patrn
PCC
ICC
Tiempo (das)
Log (ufc/g)
1.46680521900
3.335030018500
3.897400522300
4.25103080282.57088161511.1505149978
4.93425061993.22584760370
Patrn
PN
IN
Tiempo (das)
Log (u