28 al 30 de mayo de 2014 villahermosa tabasco; méxico · m. en a. rubicel cruz romero secretario...

28 al 30 de mayo de 2014Villahermosa Tabasco; México

Primera Edición, 2014División Académica de Ciencias BiológicasUniversidad Juárez Autónoma de Tabasco

Kilómetro 0.5 de la carretera Villahermosa-CárdenasEntronque a Bosques de Saloya. CP. 86039Villahermosa, Centro; Tabasco.

(993) 358 1500 extensión 6400 www.dacbiol.ujat.mx [email protected]

Queda prohibida la reproducción parcial o total del contenido de la presente obra, sin contar previamente con la autorización expresa y por escrito del titular.

Impreso y hecho en México.

Directorio UniversitarioDr. José Manuel Piña GutiérrezRector

Dra. Dora María Frias MárquezSecretaria de Servicios Académicos

Dr. Wilfrido Miguel Contreras SánchezSecretario de Investigación, Posgrado y Vinculación

M. en A. Rubicel Cruz RomeroSecretario de Servicios Administrativos

L.C.P. Marina Moreno TejeroSecretaria de Finanzas

DACBiolM.C.A. Rosa Martha Padrón LópezDirectora

Dr. Carlos Alfonso Álvarez GonzálezCoordinador de Investigación y Posgrado

M. en C. Andrés Arturo Granados BerberCoordinador de Docencia

M. en A. Arturo Enrique Sánchez MaglioniCoordinador Administrativo

M.C.A. Otilio Méndez MarinCoordinador de Difusión Cultural y Extensión

DAMRiosM.T.E. Sandra Aguilar HernándezDirectora

Mtro. Fausto IV Flores CórdovaCoordinador de Investigación y Posgrado

L.I.A. Miguel Alejo AlejoCoordinador de Docencia

Mtro. Alejandro Alpuche PalmaCoordinador Administrativo

Mtra. Martha Esther May GutiérrezCoordinadora de Extensión y Servicio Social

DACADr. Roberto Flores BelloDirector

Dr. Julio Cámara CórdovaCoordinador de Investigación y Posgrado

Dra. Edith Miranda CruzCoordinadora de Docencia

M.E.E. Esther Pavón JiménezCoordinadora Administrativa

M. en C. José Nava AyalaCoordinador de Difusión Cultural y Extensión

ComitéComité Honorario

Dr. José Manuel Piña GutiérrezRector de la UJAT

Dra. Dora María Frias MárquezSecretaria de Servicios Académicos, UJAT

Dr. Wilfrido Miguel Contreras SánchezSecretario de Investigación, Posgrado y Vinculación, UJAT

M. en A. Rubicel Cruz RomeroSecretario de Servicios Administrativos, UJAT

L.C.P. Marina Moreno TejeroSecretaria de Finanzas, UJAT

M.C.A. Rosa Martha Padrón LópezDirectora de la División Académica de Ciencias Biológicas, UJAT

M.T.E. Sandra Aguilar HernándezDirectora de la División Académica Multidisciplinaria de los Ríos, UJAT

Dr. Roberto Flores BelloDirector de la División Académica de Ciencias Agropecuarias, UJAT

Comité CientíficoDra. Jeane Rimber Indy (DACBiol-UJAT)Presidente

Dr. Eudes de Souza Correia (UFRPE)Vicepresidente

Dra. María de Lourdes Jiménez Badillo(UV-ICMP)Dr. Armando Wakida Kusunoki(CRIP-INAPESCA)Dr. Alfonso Castillo Domínguez(DAMRios-UJAT)Dr. José Ulises González de la Cruz(UJAT)M.C. Luis Enrique Amador del Ángel(CICA-UNACAR)Dra. Martha Patricia Hernández Vergara(ITBOCA)Dra. María Concepción de la Cruz Leyva(UJAT)

Comité OrganizadorDr. Carlos A. Álvarez González (DACBiol-UJAT)Presidente

Dra. Martha A. Perera García (DAMRios-UJAT)Vicepresidente

Dr. Rafael Martínez GarcíaDra. Ena Edith Mata Zayas Dra. Susana Camarillo Coop Dr. Carlos A. Cuenca SoriaM.C. Arlette Amalia Hernández FranyuttiM.C. Salomón Paramo DelgadilloM.C. Raúl E. Hernández GómezM.C. Metodio Nicolás Vite García Mtro. Jorge Víctor Mendiola Campuzano Mtro. Gilberto Eduardo Domínguez GarcíaM.C.A Otilio Méndez Marín L.C. José Juan Almeida GarcíaBiól. Fernando Rodríguez QuevedoC. Juan Pablo Quiñonez Rodríguez

Programa de ActividadesActivities Program

1. 11

Hora / Time08:30 - 09:30 Registro Registration

09:30 - 10:30 Ceremonia de Inauguración Opening Ceremony

Sesión / Session1

Biología y Taxonomia Biology and TaxonomyModerador Moderator: Dra. Martha Alicia Perera García

Hora / Time Ponencias Magistrales Keynote Presentations

10:30 - 11:00 REVIEW ON THE DEVELOPMENT OF CLOSED AQUCULTURE SYSTEM FOR GIANT FRESHWATER PRAWN (Macrobrachium rosembergii de Man) CULTURE IN INDONESIADr. Gede Suantika; Bandung Institute of Technology. Indonesia

11:00 - 11:30 THE POTENTIALS OF CHIRONOMID LARVAE (Chironomus major) AS LIVE FOOD FOR FRESH WATER PRAWN (Macrobrachium rosenbergii)Prof. Dr. Tati Suryati Syamsudin; Bandung Institute of Technology. Indonesia

11:30 - 12:00 EXPERIENCIAS CON LARVICULTURA DE Macrobrachium carcinus (CAMARÓN PITÚ) EN LARVI AQUICULTURA & PROJETOS LTDA, SITUADA EM LA REGIÓN NORESTE DEL BRASILDr. Jacqueline de Medieros; Larvicultura y Projecto, LTDA. Brasil

12:00 - 12:15 Café Coffee Break

Moderador Moderator: Dr. Alfonso Castillo Domínguez

12:15 - 12:30 PHYSIOLOGY STUDIES, EMBYROS AND LARVAL PERFORMANCE OF Macrobrachium carcinus FROM TABASCO, MEXICOJeane Rimber Indy; Universidad Juárez Autónoma de Tabasco. México

12:30 - 12:45 COMPARACIÓN MORFOMETRICA DE LOS LANGOSTINOS DEL GENERO Macrobrachium CON DESARROLLO LARVAL ABREVIADO EN LA CUENCA DEL RÍO GRIJALVA, MEXICOAntonio Chale Chim; Universidad de Quintana Roo. México

12:45 - 13:00 DESCRIPCIÓN EXTERNA DEL DESARROLLO EMBRIONARIO DE Macrobrachium carcinusMartha Alicia Perera García; Universidad Autónoma de Tabasco. México

13:00 - 13:15 LARVAL PERFORMANCE OF Macrobrachium carcinus IN DIFFERENT REARING SYSTEMSEudes de Souza Correia; UFREP. Brasil

13:15 - 13:30 INFLUENCE OF DIETARY PROTEIN LEVELS ON GROWTH PERFORMANCE AND BODY COMPOSITION OF BROODSTOCK FRESHWATER PRAWN Macrobrachium carcinus (L. 1758)Mario Alfredo Benítez Mandujano; Universidad Juárez Autónoma de Tabasco. México

13:30 - 15:15 Comida Lunch

28Miércoles / Wednesday

1. 12

Sesión / Session2

Biología y Taxonomia Biology and TaxonomyModerador Moderator: Dr. Carlos Alfonso Álvarez González


15:15 - 15:30 CHARACTERISTIC OF MALES AND FEMALES OF Macrobrachium carcinus AND M. americanum FROM TABASCO AND CHIAPAS, MEXICOK. V. Jayachandran; Kerala University of Fisheries and Ocean Sciences. India

15:30 - 15:45 LOS LANGOSTINOS DULCEACUÍCOLAS DE LA REGIÓN COSTA DE OAXACA Y LA CONDICIÓN ACTUAL DEL Macrobrachium tenellumDe los Santos Romero Rodolfo Benigno; CIIDIR. México

15:45 - 16:00 ANÁLISIS DE LA CALIDAD HIGIÉNICA E IDENTIFICACIÓN DE Aeromonas salmonicida A PARTIR DE LANGOSTINO PRIETO (Macrobrachium acanthurus)Estrada Andrade Laura Fabiola; Universidad Juárez Autónoma de Tabasco. México

16:00 - 16:15 ECOLOGÍA DE LANGOSTINOS (Macrobrachium) EN LOMA BONITA OAXACAFelipe Becerril Morales; Universidad del Papaloapan. México

16:15 - 16:30 LOS LANGOSTINOS DEL GENERO Macrobrachium CON DESARROLLO LARVAL ABREVIADO EN MEXICOLuis M. Mejía-Ortiz; Universidad de Quintana Roo. México

16:30 - 16:45 IMPACTO DE BARRERAS ANTRÓPICAS SOBRE LA MIGRACIÓN CORRIENTE ARRIBA DEL LANGOSTINO ANFÍDROMO Macrobrachium tenellum (SMITH 1871)María Carolina Rodríguez; Universidad de Guadalajara. México

16:45 - 17:00 PATRONES MORFOMÉTRICOS Y ESTRATIFICACIÓN SOCIAL EN MACHOS SILVESTRES DE Macrobrachium tenellum(SMITH, 1871)Miguel Ángel Rubio-Padilla; Universidad de Guadalajara. México

17:00 - 20:00 Cocktail

28Miércoles / Wednesday

1. 13

Sesión / Session2

Biología y Taxonomia Biology and TaxonomyModerador Moderator: Dra. Jeane Rimber Indy


09:00 - 09:30 WASTE MANAGEMENT IN RECIRCULATING AQUACULTURE SYSTEMSDr. Jacob Van Rijin; Hebrew University of Jerusalem. Israel

09:30 - 10:00 THE RESEARCH NETWORK FOR FARMING THE AMAZON RIVER PRAWN Macrobrachium amazonicumDr. Wagner Valenti; Sao Paulo State University. Brazil

10:00 - 10:30 THE FIRST IMPLEMENTATION OF RNA-INTERFERENCE IN AQUACULTURE: ALL MALE CULTURE OF PRAWNSDr. Amir Sagi; Ben Gurion University. Israel


Moderador Moderator: Dra. María Concepción de la Cruz Leyva

10:45 - 11:00 ESTRUCTURA Y CRECIMIENTO DE LA POBLACIÓN DEL LANGOSTINO (Macrobrachium tenellum) EN RIO SAN PEDRO, NAYARIT DURANTE 2003Pablo Alejandro Pérez Velázquez; Centro Regional de Investigación Pesquera. México

11:00 - 11:15 ASPECTOS BIOLÓGICO-PESQUEROS DEL RECURSO PIGUA Macrobrachium acanthurus(Wiegmann, 1836)EN EL RIO PALIZADA, CAMPECHE, MÉXICOCruz-Sánchez José L.; Universidad Autónoma del Carmen. México

11:15 - 11:30 DIETA Y EVALUACIÓN NUTRIMENTAL DEL CALDO BALANCEADO COMO ALIMENTO PARA LARVAS DE LANGOSTINO Macrobrachium rosenbergii EN HATCHERY COCHIN ANITING, KERALA, INDIAEzequiel Vázquez Julián; Universidad Juárez Autónoma de Tabasco. México

11:30 - 11:45 MORTALIDAD TOTAL DEL LANGOSTINO (Macrobrachium tenellum) EN RIO SAN PEDRO, NAYARIT DURANTE 2003Pablo Alejandro Pérez-Velázquez; Centro Regional de Investigación Pesquera. México

11:45 - 12:00 FAUNA CARIDEA DE LOS GÉNEROS Macrobrachium Y Atya EN UN PUNTO DEL RÍO ACAPONETA, NAYARITArmenta-Crespo M. H.; Universidad de Sinaloa. México

12:00 - 12:15 ANÁLISIS AMBIENTAL Y COMPARACIÓN MORFOMETRICA DE LOS LANGOSTINOS DEL GENERO Macrobrachium CON DESARROLLO LARVAL ABREVIADO EN LA CUENCA DEL RÍO USUMACINTA, MEXICORocío Y. Perera Pech; Universidad de Quintana Roo. México


29Jueves / Thursday

1. 14

Sesión / Session 2

Biología y Taxonomia Biology and TaxonomyModerador Moderator: Dr. Eudes de Souza Correia


12:30 - 12:45 FECUNDITY AND FERTILITY IN A FRESHWATER POPULATION OF THE NEOTROPICAL AMPHIDROMOUS SHRIMP Macrobrachium acanthurus FROM THE SOUTHEASTERN ATLANTICGiovana Bertini; Universidade Estadual Paulista - UNESP. Brasil

12:45 - 13:00 BIODIVERSIDAD DE LOS CAMARONES DE AGUA DULCE EN EL RÍO MANDIRA, SUR DEL ESTADO DE SÃO PAULO, BRAZILBianca Fukuda; Universidade Estadual Paulista - UNESP. Brasil

13:00 - 13:15 EFECTO DE LOS PARÁMETROS AMBIENTALES EN EL CRECIMIENTO DE LAS LARVAS DE Macrobrachium carcinusCarolina Melgar Valdes; Universidad Juárez Autónoma de Tabasco. México

13:15 - 13:30 CRUSTÁCEOS DECÁPODOS DE AGUA DULCE DE LOS RÍOS SIETE ALTARES Y LAS ESCOBAS, IZABAL. GUATEMALAJuan Carlos Tejeda Mazariegos; Universidad de San Carlos de Guatemala - USAC. Guatemala


Sesión / Session 3

Genética y Nutrición Genetics and NutritionModerador Moderator: Dr. José Ulises González de la Cruz


15:30 - 15:45 EVALUACIÓN DE DIETAS ISOPROTEICAS EN EL CULTIVO DEL LANGOSTINO Macrobrachium tenellum PARA EL SURESTE MEXICANODe los Santos Romero Rodolfo Benigno; CIIDIR. México

15:45 - 16:00 DIMORFISMO SEXUAL EN LA PLEURA ABDOMINAL DE Macrobrachium acanthurus(CRUSTACEA: DECAPODA: NATANTIA)T. Zúñiga-Marroquín; Universidad del Papaloapan. México

16:00 - 16:15 REQUERIMIENTO OPTIMO DE PROTEÍNA EN EL CRECIMIENTO Y SUPERVIVENCIA DE JUVENILES DEL LANGOSTINO DE RÍO, Macrobrachium americanum (Bate, 1869)Juan Carlos Pérez-Rodríguez; CIBNOR. México


16:30 - 17:00 Sesión de Carteles Poster Session

29Jueves / Thursday

1. 15

Sesión / Session 3

Genética y Nutrición Genetics and NutritionModerador Moderator: Dr. Gopal Krishna


17:00 - 17:15 CONOCIMIENTO ACTUAL SOBRE FECUNDIDAD Y FERTILIDAD EN LANGOSTINOS DEL GÉNERO Macrobrachium EN AMÉRICA: ¿HACIA DÓNDE DIRIGIR LA INVESTIGACIÓN?Vargas-Ceballos M.; Universidad de Guadalajara. México

17:15 - 17:30 INCLUSIÓN DE CÁLICES DE Hibiscus sandariffa EN DIETAS EXPERIMENTALES PARA JUVENILES DE Macrobrachium tenellum: EFECTOS EN EL ÍNDICE Y PIGMENTACIÓN DE CROMATÓFOROSMartín Alonso Aréchiga Palomera; Universidad de Guadalajara. México

17:30 - 17:45 MODELO DEL DESARROLLO LARVARIO DE Cryphiops caementarius: PALAEMONIDO AMFIDROMO DE AGUAS CONTINENTALES DE CHILEMa. Cristina Morales; Universidad Católica del Norte. Chile

17:45 - 18:00 EFECTO DE LA TEMPERATURA DE CULTIVO EN LA SUPERVIVENCIA Y CRECIMIENTO DE LARVAS DEL LANGOSTINO DE RÍO, Macrobrachium americanum (DECAPODA: PALAEMONIDAE) ALIMENTADAS CON UNA DIETA MIXTAStig Yamasaki Granados; CIBNOR. México

18:00 - 18:15 LA DIGESTIBILIDAD DE ALIMENTOS EN LANGOSTINOS DEL GÉNERO Macrobrachium COMO ESTRATEGIA PARA FORMULAR ALIMENTOS EFICIENTES Y AMIGABLES CON EL AMBIENTEMontoya Martínez C.; Universidad de Guadalajara. México

29Jueves / Thursday

1. 16

30Viernes / Friday

Sesión / Session3

Genética y Nutrición Genetics and NutritionModerador Moderator: M.C.A. Rosa Martha Padrón López


09:00 - 09:30 Macrobrachium rosenbergii FARMING IN INDIA: A SUCCESS STORYDr. W.S. Lakra; Central Institute of Fisheries and Education. India

09:30 - 10:00 GLOBAL STATUS OF THE FRESHWATER PRAWN FARMING INDUSTRYDr. C. Mohanakumaran Nair; Kerala University of Fisheries and Ocean Sciences. India

10:00 - 10:30 WATER QUALITY MANAGEMENT IN Macrobrachium HATCHERIESDr. A. K. Reddy; Central Institute of Fisheries and Education. India


10:45 - 11:00 DIPLOID CHROMOSOME NUMBER OF FRESHWATER PRAWN Macrobrachium carcinus AND Macrobrachium sp.(DECAPOD, PALAEMONIDAE) FROM TABASCO, MEXICOJeane Rimber Indy; Universidad Juárez Autónoma de Tabasco. México

11:00 - 11:15 MOLECULAR DETECTION OF THE BACTERIAL COMMUNITY IN THE DIGESTIVE SYSTEM OF THE FRESHWATER PRAWN Macrobrachium carcinusJ. Ulises González de la Cruz; Universidad Juárez Autónoma de Tabasco. México

Sesión / Session4

Biotecnología y Acuatultura Biotechnology and AquacultureModerador Moderator: Dr. A. K. Reddy


11:15 - 11:30 FECUNDIDAD DEL Macrobrachium acanthurus (Wiegmann, 1836)EN EL RIO PALIZADA, CAMPECHECruz-Sánchez José L.; Universidad Autónoma del Carmen. México

11:30 - 11:45 ELIMINACIÓN DEL ÓRGANO X EN EL CAMARÓN Macrobrachium americanum COMO UN PROMOTOR DEL ACELERAMIENTO DE LA REPRODUCCIÓNSainz- Hernández Juan Carlos; CIIDIR. México

11:45 - 12:00 PRODUCTIVIDAD Y FLUJOS DE COMUNICACIÓN ENTRE INVESTIGADORES Y GRUPOS DE INVESTIGACIÓN RELACIONADOS CON EL ESTUDIO DEL GÉNERO MacrobrachiumChong Carrilo, O.; Universidad de Guadalajara. México

12:00 - 12:15 LOS LANGOSTINOS DEL GENERO Macrobrachium CON INTERÉS ECONÓMICO EN AMÉRICA LATINAMarcelo García Guerrero; Instituto Politécnico Nacional. México


1. 17

30Viernes / Friday

Sesión / Session4

Genética y Nutrición Genetics and NutritionModerador Moderator: Dr. Eudes de Souza Correia


12:30 - 12:45 CHARACTERISATION OF LACTIC ACID BACTERIA ISOLATED FROM THE DIGESTIVE SYSTEM OF THE PRAWN Macrobrachium carcinusJ. Ulises González-de la Cruz; Universidad Juárez Autónoma de Tabasco. México

12:45 - 13:00 EYESTALK ABLATION PROCEDURES TO MINIMICE PAIN IN THE FRESHWATER PRAWN Macrobrachium americanumSainz-Hernández Juan Carlos; CIIDIR. México


Moderador Moderator: Dr. Armando Wakida Kusunoki

14:00 - 14:15 TRANSFERENCIA DE TECNOLOGÍA PARA DESARROLLAR EL POLICULTIVO TILAPIA-LANGOSTINO EN EL ESTADO DE MORELOS, MÉXICO Alberto Asiain; Colegio de Posgraduados. México

14:15 - 14:30 PRODUCTION POTENTIAL OF Macrobrachium rosenbergii VIS-À-VISADVANCED BIOTECHNOLOGICAL TOOLSGopal Krishna; CIFE. India

14:30 - 14:45 EFECTO DE TRES SALINIDADES EN EL DESARROLLO LARVAL Y SUPERVIVENCIA DE Macrobrachium carcinus (Linnaeus, 1756)Heradia Pascual-Cornelio; Universidad Juárez Autónoma de Tabasco. México

14:45 - 15:00 EL RECURSO ACUÍCOLA-PESQUERO LANGOSTINO (Macrobrachium sp) EN LA ZONA CENTRAL DE VERACRUZ: ANÁLISIS DE LA CADENA PRODUCTIVARodrigo Gómez Rigalt; Colegio de Posgraduados. México

15:00 - 15:15 COMMERCIAL SEED PRODUCTION OF FRESHWATER PRAWNS – PROSPECTS AND CHALLENGESK. R. Salin; Kerala University of Fisheries and Ocean Studies. India

15:15 - 15:30 MORPHOMETRIC CHANGES OF THE EGGS BY EFFECT OF TEMPERATURE DURING THE EGGS DEVELOPMENT IN THE PRAWN Macrobrachium americanumSainz-Hernández Juan Carlos; CIIDIR. México

15:30 - 15:45 ¿ES EL GENERO Macrobrachium UN POSIBLE CONTROL BIOLÓGICO DE LARVAS DE Aedes Aegypti?Vargas-Ceballos M. A.; Universidad de Guadalajara. México

1. 18

30Viernes / Friday

Sesión / Session5

Manejo de agua, sanidad acuícola y Biofloc Water management, aquatic health and BioflocModerador Moderator: M.C. Raúl E. Hernández Gómez


15:45 - 16:00 CRECIMIENTO EN MACHOS Y HEMBRAS DEL LANGOSTINO Macrobrachium tenellum (Decápoda: Palaemonidae) EN ESTANQUES RÚSTICOS EN EL ESTADO DE NAYARIT, MEXICOMario Alfredo Benítez Mandujano; Universidad Juárez Autónoma de Tabasco. México

16:00 - 16:15 BICULTIVO EN BIOFLOC DE LANGOSTINO MALAYO (Macrobrachium rosenbergii) Y TILAPIA (Oreochromis niloticus), BAJO DOS ESQUEMAS DE PRODUCCIÓNDe la Cruz Neri Obed; Instituto Tecnológico de Boca del Ríos. México

16:15 - 16:30 EFECTO TÓXICO RESIDUAL DE JUVENILES DE Macrobrachium tenellum ENVENENADOS CON COUMAFOSKaren Noemí Nieves Rodríguez; Universidad de Guadalajara. México

16:30 - 16:45 TRATAMIENTO Y CALIDAD DE AGUA EN Macrobrachium rosenbergii EN HATCHERY COCHIN ANITING, KERALA, INDIAEzequiel Vázquez Julián; Universidad Juárez Autónoma de Tabasco. México

16:45 - 17:00 REPRODUCTION AND THE IMMUNE SYSTEM IN TH FRESHWATER FEMALES PRAWN Macrobrachium americanumJuan Carlos Sainz-Hernández; CIIDIR. México

17:00 - 17:15 AVANCES DEL DESARROLLO LARVAL DE Macrobrachium carcinus (Linnaeus, 1756) EN LA DAMRViridiana Guadalupe Zetina de la Cruz; Universidad Juárez Autónoma de Tabasco. México

17:30 - 18:00 Premiación de Carteles Poster Prizeoster Session18:00 - 18:30 Ceremonia de Clausura Closing Ceremony20:00 - 23:00 Cena de Clausura Closing Dinner

Sesión de CartelesPoster Session

1. 21

Sesión de Carteles Poster SessionModerador Moderator: M.C. Nicolás Vité

1 COMPARACIÓN MORFOMETRICA DE LOS LANGOSTINOS DEL GENERO Macrobrachium CON DESARROLLO LARVAL ABREVIADO EN LA CUENCA DEL RÍO GRIJALVA, MEXICOAntonio Chale Chim; Universidad de Quintana Roo. México

2 FAUNA CARIDEA DE LOS GÉNEROS Macrobrachium Y Atya EN UN PUNTO DEL RÍO ACAPONETA, NAYARITArmenta-Crespo M. H.; Universidad Autónoma de Sinaloa. México

3 EFECTO DE DIFERENTES INTENSIDADES DE LUZ SOBRE EL CRECIMIENTO, SUPERVIVENCIA Y EXPRESIÓN DE CROMATÓFOROS EN JUVENILES DE Macrobrachium tenellumArrona-Ortiz, J.; Universidad de Guadalajara. México

4 FECUNDIDAD DEL Macrobrachium acanthurus (Wiegmann, 1836)EN EL RIO PALIZADA, CAMPECHECruz-Sánchez José L.; Universidad Autónoma del Carmen. México

5 EVALUACIÓN DE LA CONCENTRACIÓN LETAL 50 (LC50

) DE DIVERSOS COMPUESTOS EN JUVENILES DEL LANGOSTINO Macrobrachium tenellumE. Leal Cárdenas; Universidad de Guadalajara. México

6 CRECIMIENTO DEL LANGOSTINO Macrobrachium americanum A DIFERENTES TAMAÑOS DE SIEMBRA EN UN ESTANQUE SEMI-RÚSTICO EN UN SISTEMA DE JAULAS Fermín López Uriostegui; Universidad Autónoma de Nayarit. México

7 EFECTO TÓXICO RESIDUAL DE JUVENILES DE Macrobrachium tenellum ENVENENADOS CON COUMAFOSKaren Noemí Nieves Rodríguez; Universidad de Guadalajara. México

8 ENFERMEDADES DE LOS LANGOSTINOS Macrobrachium tenellum Y M. rosenbergii EN ESTANQUES RÚSTICOSJesús T. Ponce Palafox; Universidad Autónoma de Morelos. México

9 INFLUENCE OF DIETARY PROTEIN LEVELS ON GROWTH PERFORMANCE AND BODY COMPOSITION OF BROODSTOCK FRESHWATER PRAWN Macrobrachium carcinus (L.1758).Mario Alfredo Benítez Mandujano; Universidad Juárez Autónoma de Tabasco. México

10 CRECIMIENTO EN MACHOS Y HEMBRAS DEL LANGOSTINO Macrobrachium tenellum (Decápoda: Palaemonidae) EN ESTANQUES RÚSTICOS EN EL ESTADO DE NAYARIT, MEXICOMario Alfredo Benítez Mandujano; Universidad Juárez Autónoma de Tabasco. México

11 PREVALENCIA DE INFESTACIÓN DE Macrobrachium tenellum (DECAPODA, PALAEMONIDAE) POR EL PARÁSITO BRANQUIAL Probopyrus pacificensis (ISÓPODA, BOPYRIDAE) EN LA LAGUNA COYUCA, GUERRERO, MÉXICORamiro Román Contreras; Universidad Nacional Autónoma de México. México

29Jueves / Thursday

1. 22

12 ANÁLISIS AMBIENTAL Y COMPARACIÓN MORFOMETRICA DE LOS LANGOSTINOS DEL GENERO Macrobrachium CON DESARROLLO LARVAL ABREVIADO EN LA CUENCA DEL RÍO USUMACINTA, MEXICORocío Y. Perera Pech; Universidad de Quintana Roo. México

13 RELACIÓN ENTRE EL PESO Y LA PRODUCCIÓN DE ESPERMATÓFOROS EN Macrobrachium acanthurusTiago Viana Da Costa; Universidad Federal Rural del Rio de Janeiro. Brasil

14 TOXICIDAD DE CRIOPROTECTORES EN ESPERMATÓFOROS DE Macrobrachium acanthurusTiago Viana Da Costa; Universidad Federal Rural del Rio de Janeiro. Brasil

15 ¿ES EL GÉNERO Macrobrachium UN POSIBLE CONTROL BIOLÓGICO DE LARVAS DE Aedes Aegypti?Vargas-Ceballos M. A.; Universidad de Guadalajara. México

16 LETHAL SALINITY IN Macrobrachium carcinus LARVAEJoao Paulo Viana De Lima; Instituto Agronómico de Pernambuco(IPA) UFRPE-DAPAq. Brasil

17 LARVAL DEVELOPMENT OF Macrobrachium carcinus IN BRAZILJoao Paulo Viana De Lima; Instituto Agronómico de Pernambuco(IPA) UFRPE-DAPAq. Brasil

18 CARACTERÍSTICAS IMPORTANTES DEL LANGOSTINO Macrobrachium carcinus: COMO UN RECURSO CON POTENCIAL ACUÍCOLAMario Alfredo Benítez Mandujano; Universidad Juárez Autónoma de Tabasco. México

19 EFFECT OF THE BIOFLOC ADDITION FOR NURSERY PHASE OF Macrobrachium rosenbergii De Mann USING ZWD SYSTEMDea Indriani Astuti; Institut Teknologi Bandung, Bandung. Indonesia

20 BACTERIAL COMMUNITY IN ZERO WATER DISCHARGE SYSTEM (ZWD) DURING FOR NURSERY PHASE OF Macrobrachium rosenbergii De Mann Pingkan Aditiawati; Institut Teknologi Bandung, Bandung. Indonesia

29Jueves / Thursday

Resumen de Ponencias MagistralesSummary of Keynote Presentations

1. 25

WATER QUALITY MANAGEMENT INMacrobrachium HATCHERIES

Dr. A. K. ReddyPrincipal Scientiest CIFE (Central Institute of Fisheries Education), Indian Council of Agricultural Research, Panch Marg, Off Yari Road, Versova, Mumbai-400 061, India. [email protected]

Water quality plays major role in successful operation of any aqua-hatchery. Among the cultivable aquatic animals, species belongs to Macrobrachium genus have peculiar life cycle, specifically differential growth in larval stages, prolonged life cycle, food and feeding habits, etc. These characters posed challenges among the biologists.

In addition to these things, maintenance of optimum water quality parameters play key role in successful completion of life cycle, growth and survival of larvae. Macrobrachium rosenbergii is one of the major cultivable species in India. Depending on the hatchery location different sources of saline waters are in use in various parts of India. Most of the coastal hatcheries use natural seawater following flow through system.

Landlocked states and few places in the coastal states far away from the coast use either artificial seawater or brine/trucked seawater depending on the distance. Artificial seawater is prepared with commercial grade salts such as sodium chloride, magnesium sulphate, magnesium chloride, calcium chloride, potassium chloride, sodium bicarbonate, potassium bromide, boric acid and cobalt chloride. Brine water having salinity below 150 ppt is normally used in inland prawn hatcheries, because in high salinity brine (>150 ppt) most of the essential micronutrients evaporate and effects on growth and survival of larvae.

In addition to these two types waters, technology also developed for larval rearing of M. rosenbergii by using inland ground saline water. Inland saline waters invariably have low levels of potassium at many places and high levels of calcium and variable concentrations of magnesium in comparison to natural seawater. The potassium and magnesium are key ions essential for normal physiological functions of prawn larvae. The potassium levels in 12ppt natural sea water (NSW) varied between 128 and 135 ppm whereas in IGSW it ranged from 18 to 20 ppm.

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The potassium ion in the 12 ppt IGSW was fortified with Muriate of Potash (a commercial fertilizer) at par with 100% level of natural sea water (NSW).

Hardness of IGSW is very high in the form of calcium. The calcium levels in IGSW were ranged from186-198 ppm as against 140-145 ppm in NSW and magnesium levels varied between 280 to 285 ppm whereas in NSW it fluctuated from 470 to 514 ppm. The calcium and magnesium ratios play important role in osmoregulation and metamorphosis of prawn larvae. In 12 ppt IGSW the Ca:Mg ratios ranged 1:1.60 to 1:1.55 as against 1: 3.36 to 1:3.55 in NSW. In order to make it suitable, the IGSW was passed through ion-exchange resin where Ca and Mg ions were exchanged for sodium (Na+) ions. In this process along with calcium, magnesium was also reduced drastically. To maintain the Ca and Mg ions within the optimum range, the IGSW passed through ion-exchange resin was fortified with magnesium chloride and maintained at 1:3.5 ratios. Repeated experiments were conducted with various concentrations of potassium and, different ratios of calcium and magnesium. In order to utilize IGSW economically for the operation in giant freshwater prawn hatcheries, the potassium level was maintained at 50 to 60% of the NSW.Similarly the calcium and magnesium ratios maintained at 1:2.5. These levels of potassium, calcium and magnesium levels not affected the growth and survival of larvae as compared to the NSW.

In addition to the above basic water quality requirements for successful operation of prawn hatcheries, maintenance of routine day to day water quality in the rearing tanks is also play important role in larval growth and survival. The water quality parameters such as water temperature, dissolved oxygen, pH, ammonia, nitrite and nitrate are to be monitored regularly. These parameters mainly fluctuate depending on the density of larvae and nature of feed. Nitrogenous waste products are highly toxic to the larvae. Therefore, these parameters need to be maintained within the optimum range of 0.01-0.10 ppm, 0.05-0.15 ppm and 20-30 ppm for ammonia (NH3), nitrite (NO2) and nitrate (NO3) respectively. The dissolved oxygen (DO) and pH should

be maintained within the range of 5.0-6.0 ppm and 7.5-8.5 ppm respectively. The paper describes various issues related to water quality and its role on the growth and survival of M. rosenbergii larvae reared in different saline water media.

WATER QUALITY MANAGEMENT INMacrobrachium HATCHERIES

Dr. A. K. Reddy

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GLOBAL STATUS OF THE FRESHWATER PRAWNFARMING INDUSTRY

Dr. C. Mohanakumaran NairPro Vice-Chancellor KUFOS (Kerala University of Fisheries and Ocean Sciences). Kochi, India. [email protected]

Farming of freshwater prawns of the genus Macrobrachium are often adapted to sustainable aquaculture practices. The oriental river prawn M.nipponense in China and the giant river prawn M. rosenbergii in rest of the world are the two major species farmed, apart from several native species farmed on limited scale or represented in capture fisheries. Global production of farmed freshwater prawns was close to 4,61,000 mt in 2012 registering a slight increase compared to the 2009 figures by FAO. China continued to remain the leader in freshwater prawn production in 2012 contributing nearly 56% of the global farmed output of M. rosenbergii (2,23,586 mt) followed by Bangladesh, Thailand, Vietnam, Taiwan, Indonesia, Myanmar and India. Production of M. nipponense correspondingly increased by ~13% to 2,37,431 mt in 2012. One Country which registered significant increase (73%) in production of M. rosenbergii in 2012 compared to 2009 was Bangladesh.

Many other prawn species with aquaculture potential have been described based on their regional significance, chiefly M. amazonicum in Brazil, M. lar in the Pacific Island region, M. vollenhovenii in Africa, M. americnum in the Western Americas, M. lanchesteri in Southeast Asia, and M. malcolmsonii and M. gangeticum in the Indian sub-continent.

The giant river prawn is exotic to Mexico with the founder stocks introduced from Hawaii in the 1960’s. Farmed output of this prawn was reported to be 17 mt in 2011. Apart from M. rosenbergii FAO also records another category of river prawns from aquaculture, possibly including M. carcinus, M. acanthurus, M. heterochirus and M.olfersii ranging from 12 mt in 2009 to 56 mt in 2011. Much higher figures of 2455 mt of river prawns in 2012 were reported from capture fisheries.

Mass scale seed production of M. carcinus was successful in UJAT, Mexico in 2013 and further refining of the techniques to optimize

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production particularly with respect to the larval rearing period and survival rates are progressing.

In India, despite loss of farming areas to the whiteleg shrimp Litopenaeus vannamei, mostly in the State (Province) of Andhra Pradesh, the giant river prawn is increasingly used for culture-based fisheries in irrigation reservoirs. In states like West Bengal and Maharashtra large-scale exploitation from wild sources still continues, while the recent stocking of prawn seed in reservoirs have resulted in substantial boost in production. Notwithstanding the peak national production of 42,820 mt in 2005, the recent estimates show a downfall of Indian production by over 90% to 3332 mt in 2012.

Future prospects of freshwater prawns largely depend on the success of the current efforts aimed at genetic improvement, and development of all male progeny that will produce quality post-larvae for the culture industry. Organic aquaculture, integrating prawns and rice-fish crops as prevalent in many Asian countries also has the potential to steer the future course of global freshwater prawn farming towards sustainable levels.

GLOBAL STATUS OF THE FRESHWATER PRAWNFARMING INDUSTRY

Dr. C. Mohanakumaran Nair

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Macrobrachium rosenbergii FARMING IN INDIA:A SUCCESS STORY

Dr. W. S. LakraVice-Chancellor CIFE (Central Institute of Fisheries and Education), Indian Council of Agricultural Research, Versova, Mumbai, India.Phone Off. 022 26363404; Fax 022 26361573 [email protected] / [email protected]

Giant freshwater prawn farming is an age old practice in India. There are more than 100 commercially important Macrobrachium species identified from different parts of the world, where about 25 species have been identified from Indian waters. Among the available species in India, Macrobrachium rosenbergii (Scampi) became popular because of its economic traits such as fast growth rate, mature and breeding under captive conditions, omnivorous feeding habit, high nutritive value, ready demand in both domestic and export markets and its suitability to culture along with carps. India is blessed with enormous natural resources for M. rosenbergii and the species is reported from almost all the major rivers. The stocks collected from different geographical locations show wide variation in their growth pattern. This indicates the possibility of wide genetic variation among the stocks of different river systems.

Although M. rosenbergii culture in India was noticed in 1950s, but its true commercial farming has been taken up in the late 1990s only. The production steadily increased from less than 500 tons in 1995 to 43, 000 tons in 2005 making India at third position in the world. Later, the production gradually declined to 4, 000 tons during 2010-11. The drastic decline in scampi production has been attributed to the use of poor quality of seed, slow growth rate, disease outbreaks, high input cost, etc.

The Central Institute of Fisheries Education has been working on various aspects of hatchery and grow-out technologies since 1980 and has developed proven technologies which were demonstrated in different agro-climatic regions of India. As the giant freshwater prawn needs certain amount of saline water to complete its life-cycle, CIFE has developed a package of practices by using artificial sea water.

This technology was basically developed for the establishment of hatcheries in the land locked states and areas away from the

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sea coast. Very recently, small scale hatcheries having production capacity ranging 1.0-2.0 million post larvae /annum have been established in the North and North Eastern States of India i.e., Tripura, Manipur, Nagaland, Assam, Mizoram, Madhya Pradesh and Uttar Pradesh. The establishment of these hatcheries encouraged the farmers to undertake monoculture of prawns and poly culture of carps and prawns in these states. A production ranging from 1150 to 1500kg/ha/8 months and 450-600 kg prawn and 2600 to 3500 kg fish / ha/ year has been achieved in mono and poly culture respectively.

A technology has also been developed for establishment of giant freshwater prawn hatcheries by using ground saline water in Rajasthan and Haryana states. The quality of inland ground saline water (IGSW) is quite different than the natural sea water particularly in the ionic composition. The IGSW is deficient in potassium, high with hardness and variable concentrations of Ca and Mg ions.

A programme on genetic improvement through selective breeding is in progress at Central Institute of Freshwater Aquaculture in collaboration with World Fish Centre, Malaysia. The Central Institute of Fisheries Education has also contributed towards genetic studies on this species. A technology developed in Israel for production of all male population will be a boon in M. rosenbergii farming globally. Mono sex culture of all male population is in progress at certain selected farms in India.

The authors highlight the progress in research, technology and capacity development in M. rosenbergii farming in India.

Macrobrachium rosenbergii FARMING IN INDIA:A SUCCESS STORY

Dr. W. S. Lakra

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Dr. Amir SagiDepartment of Life Sciences and the National Institute for Biotechnology in the Negev. Ben-Gurion University of the Negev. P.O. Box 653, Beer Sheva 84105. Israel. [email protected]

THE FIRST IMPLEMENTATION OF RNA-INTERFERENCE IN AQUACULTURE:ALL MALE CULTURE OF PRAWNS

Monosex is a desirable practice in aquaculture. Differences between males and females of the same species, in growth rate, alimentary needs and behavioral patterns, dictate the need to establish management procedures specifically adjusted to one sex or the other. In one of the most economically important cultured freshwater species, the prawn Macrobrachium rosenbergii, males grow faster and reach larger size at harvest than females thus all-male culture is desired.

Temporal knockdown (gene silencing) of an insulin like androgenic gland encoding gene (IAG), using RNA interference (RNAi), in M. rosenbergii has recently enabled the alteration of the phenotypic sex of genetic males into functional 'neo-females' capable of producing an all-male progeny. This is the first instance of an aquaculture commercial use of a monosex population derived from a single gene silencing-induced sex reversal.

Safety considerations to the use of RNAi in commercial aquaculture practices were demonstrated through clearance of non-specific exogenous dsRNA and recovery of Mr-IAG expression following dsMr-IAG injection in a time dependent manner. dsRNA is present in the prawn hemolymph and cells up to three days post administration and is fully cleared thereafter. Mr-IAG transcript level was reduced by 97.4% three days post administration and was almost fully recovered (87% of control) 28 days post administration.

The neo-females generated through Mr-IAG silencing show normal female reproductive behavior and reproductive development as compared to a female fraction of a normal mix population.

The generated all-male progeny populations showed similar social structure compared to a males fraction of a normal mix populations.

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Based on the above RNAi-based biotechnologies represent a secure and environmentally safe methods eliminating the need for exogenous hormones or other chemicals and does not pose genetic modifications (non-GMO). Thus, it is most likely that RNAi-based biotechnologies will be widely used in different aspects of the aquaculture industry, and in particular to crustacean aquaculture in the future.

Dr. Amir Sagi

THE FIRST IMPLEMENTATION OF RNA-INTERFERENCEIN AQUACULTURE: ALL MALE CULTURE OF PRAWNS

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Dr. Jaap Van RijnThe Robert H. Smith Faculty of Agriculture, Food and Environment. The Hebrew University of Jerusalem. P.O. Box 12, Rehovot. Israel. [email protected]

WASTE MANAGEMENT IN RECIRCULATINGAQUACULTURE SYSTEMS

Waste management in aquaculture depends to a large extent on the type of culture method used. Whereas, in some systems, such as conventional earthen-bottom ponds, waste products do not accumulate due to the fact that fish density does not exceed the natural carrying capacity of the system, in other culture systems, such as open raceways, highly diluted waste is produced, which often cannot be treated. In RAS, due to a reduction in water use, concentrated solid and dissolved wastes are produced. Such concentrated wastes can be treated and thus allow for a considerable reduction in waste discharge as compared to more open culture systems.

Waste reduction methods in RAS are either based on end-of-pipe treatment of the concentrated effluent waste, are an integrated part of the water recirculation loop of such systems, or are a combination of both. In addition, other management practices in RAS such as using high quality feeds, applying controlled feeding regimes to minimize accumulation of unused feed and using sophisticated methods for water quality monitoring and control, all result in more efficient fish growth, hence, waste reduction.

End-of-pipe treatment of solid and dissolved aquaculture wastes from freshwater RAS is similar to that of treatment methods used in other animal husbandry practices. Among these methods are: direct land application of aquaculture waste, treatment of waste by regional/municipal wastewater treatment plants or by on-site systems such as aerobic and anaerobic lagoons, constructed wetlands and composting facilities. In marine RAS, effluent treatment is more restricted than in freshwater systems and here natural and constructed wetlands are sometimes used.

In addition, some marine RAS systems are operated as part of a polyculture system in which fish culture effluent is used for production of valuable byproducts such as seaweed and bivalves. Waste management within RAS is often limited to conversion

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of ammonia to nitrate by nitrification and CO2 removal by

degassing. Thus, except for part of the carbon, all other elements excreted by the fish accumulate in these systems and require removal by water exchange and solids capture. Additional waste treatment procedures within the reuse water flow of commercial RAS systems are used to a limited extent. An example of a RAS operated with additional waste treatment steps is a system in which sludge is retained in order to induce many of the natural processes occurring in the sediment of conventional fish ponds.

In this system, sludge is concentrated by means of settling or mechanical filtration and kept in treatment basins that are part of the reuse water flow of the RAS. Like in anaerobic, organic-rich sediments, gasification of nitrogen and carbon as well as bacterial-mediated phosphorus mineralization reduce the waste production in these RAS to such an extent that water and waste discharge are very low or not required.

Dr. Jaap Van Rijn

WASTE MANAGEMENT IN RECIRCULATINGAQUACULTURE SYSTEMS

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Dr. Wagner Cotroni ValentiSão Paulo State University - UNESP, Coastal Campus, São Vicente, SP, Brazil. [email protected]

THE RESEARCH NETWORK FOR FARMINGTHE AMAZON RIVER PRAWN

Research Programs for farming wild species is essential to introduce new species in aquaculture. These should comprise all phases of the productive process. In this paper, we will describe how we have conceived the network to develop technology for farming the Amazon river prawn, Macrobrachium amazonicum, and the main results.

A comprehensive grid of knowledge necessary to produce M. amazonicum was defined and experts in each topic from different institutions were invited to work together. Thus, a multidisciplinary and multiinstitutional research network was established in 2001. This program has been developed in Brazilian universities and research institutes. It has been conceived to cover all elements of the productive chain and consider the economic and social aspects, the environmental impact and animal welfare.

Currently, there is available technology for all phases of production, which matchs the principles of responsible aquaculture. Broodstock may be easily maintained in outdoor ponds or indoor tanks, stocked at 40-50/m2 and fed on commercial aquaculture diets. Hatchery tanks may be stocked at 120-140 larvae/L, while nursery tanks at 10-15 post-larvae (PL)/L. Productivity may attain more than 70 PL/L in 18 days and 15 0.1g juveniles/L in 15-30 days. Although Artemia nauplii are essential during hatchery, there is a clear preference for inert diet during most of the larval cycle. In tropical regions, grow-out ponds may be stocked at 80-100 PL/m2 and survival is about 70%, while productivity may surpass 5,000 kg/ha/yr. Apparent feed conversion rate is about 3.0:1.

The main constraints for commercial aquaculture are the low fecundity, early female maturation (which decreases growth rate), the male heterogeneous growth, the small size at harvest and the high apparent feed conversion rate. Thus, substantial research should be conduct for improving culture performance.

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Dr. Gede SuantikaMicrobial Biotechnology Research Group, School of Life Science and Technology. Bandung Institute of Technology. Indonesia. [email protected]

REVIEW ON THE DEVELOPMENT OF CLOSED AQUCULTURE SYSTEMFOR GIANT FRESHWATER PRAWN (Macrobrachium rosenbergii de Man)

CULTURE IN INDONESIA

IntroductionThe giant freshwater prawn (Macrobrachium rosenbergii de Man) is one of the main potential fishery commodities that has high economic value in Indonesia. Nowadays, most of the prawn productions are utilizing primarily extensive earthen ponds with static culture, which use minimal inputs of fertilizer, or feeds, commonly based on the pond's natural productivity and the solar energy. The main problem of this culture system is to maintain water quality due to the accumulation of toxic ammonium (NH4+) as the product of decomposition process from the prawn excretion and/or uneaten feed. This phenomenon can results in low stocking density and survival rate of the prawn culture, besides other problems from the prawn benthic characteristics and cannibalism behavior.

All of these conditions contributed to the low productivity of the prawn culture. Study on the prawn production efficiency can be done through the improvement of water quality management and the increment of surface territorial area of the prawn culture to reduce prawn cannibalism. It is known that there is a strong linear relationship between primary productivity and prawn yields in earthen ponds. This research was aimed to explore the intensification of prawn culture in an ecological, social and economic sustainable way, using the periphyton biofilter technology which is based on the development of dense microorganisms that both function as a bioreactor controlling water quality as well as a protein food source for the prawn. This research also explored the hypotheses that using natural periphyton substrate and the application of supplemental feeding in periphyton-based ponds can improve water quality, productivity, and increase the surface territorial area of the prawn to further increase the prawn survival rate and productivity, by conducted an research on optimization of total surface area of periphyton substrate to enhance the periphyton development.

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different substrate densities of 2 and 4 bamboo substrate shelter structures (0.6x0.6x0.2 m3) per pond, respectively. Post larvae prawns were stocked at the stocking density of 30 PL40 (40 old-day post larvae) prawns per square meter for control and 60 PL40 prawns per square meter for the others. The third experiment was aimed to know the effect of nitrifying bacteria and microalgae Chlorella sp. as periphyton biofiltration agents combined with 3-dimensional cubical bamboo shelter as periphyton attached substrate and shelter on nursery of post larvae stage in commercial outdoor pond condition. This experiment was carried out in 9 ponds each with 10 m² area and average depth 0.8 m at the outdoor prawn pond, for a period of 49 days. The experiment was conducted using 3 treatments with 3 replications for each treatment. In this experiment, the prawn culture with or without the addition of periphyton substrate shelter will be compared.

The culture without the addition of shelter was used as control treatment. The addition of substrate will be done by following two different substrate densities (substrate surface area index/SAI) of: 20%, and 40% of ponds total surface area. For the SAI of 20% and 40%, two and four bamboo substrate shelter structures (0.9x0.9x0.6 m3) were installed per square meter of pond’s surface area, respectively. Stimulation of periphyton and phytoplankton development was obtained by inoculation of nitrifying bacteria and Chlorella sp. microalgae culture at the rate of 105 CFU.ml-1 and 104 cells.ml-1 every two weeks, respectively. Post larvae prawns were stocked by 90 PL20 (20 old-day post larvae) prawns per square meter for control and 180 PL20 prawns per square meter for the other treatments. Fourth experiment was conducted to study the effect of the optimization of prawn stocking density on grow out using the periphyton biofiltration based rearing system.

This experiment was carried out in 5 ponds each with 24 m2 areas representing five different prawn stocking densities, for culture period of 60 days. The experiment was divided into 5 treatments with stocking density of 30, 40, 50, 60, and 70 juvenile prawns per square meter. For this experiment, three periphyton carpet substrates (1x1 m2) were placed in each grow out pond.

The use of periphyton biofilter was to obtain optimum environmental conditions and to increase the water use efficiency as well as the nutrient / feed protein utilization, leading to higher productivity of pond culture, reduction in overall production costs, and improve overall sustainability of prawn pond culture production.

To achieve the goals, this research was conducted by these following experiments. First experiment to examine the effect of nitrifying bacteria addition combined with the use of 3-dimensional cubical bamboo substrate as substrate for periphyton attachment and shelter on Macrobrachium rosenbergii prawn post larvae nursery in lab condition, the second in indoor hatchery, and the third in outdoor pond. Fourth experiment was conducted to study the effect of the optimization of prawn stocking density on grow out using the periphyton biofiltration based rearing system.

Experimental DesignFirst experiment was conducted in 12 ponds each with 0.24 m² area and average depth 0.4 m at the indoor prawn hatchery for 60 days culture period using four different treatments with three replicas each. In this experiment, the prawn culture with or without the addition of periphyton substrate shelter will be compared. The culture without the addition of shelter was used as control treatment. The addition of shelter will be done following three different substrate densities of 2, 4, and 8 bamboo substrate shelter structures (20 x 10 x 6,5 cm3) per pond, respectively. Post larvae prawns were stocked at the stocking density of 30 PL40 (40 old-day post larvae) prawns per square meter for control and 60 PL40 prawns per square meter for the others. Second experiment was conducted in 9 ponds each with 0.72 m² area and average depth 0.4 m at the indoor prawn hatchery for 28 days culture period using three different treatments with three replicas each. In this experiment, the prawn culture with or without the addition of periphyton substrate shelter will be compared.

The culture without the addition of shelter was used as control treatment. The addition of shelter will be done following two

Dr. Gede Suantika

REVIEW ON THE DEVELOPMENT OF CLOSED AQUCULTURE SYSTEM FOR GIANT FRESHWATER PRAWN

Macrobrachium rosenbergii de Man) CULTURE IN INDONESIA

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Sinking feed for prawn was applied daily in all experiments as supplemental feed at the rates of 5% of prawn biomass. Water and sediment quality, bacteria, periphyton and plankton quality, and prawn yield parameter of each treatment were compared and statistically analyzed for all experiments.

Results and DiscussionsFrom the first experiment, optimum performance was acquired in the culture with the addition of four “shelters” with weight, length, and biomass, respectively (1,00 ± 0,04) grams, (4,91 ± 0,03) cm, and (63,17 ± 4,27) grams. From the measurement of physical-chemical parameters obtained, ammonium ranged from 0.033 to 0.288 mg.L-1, nitrite from 0.011 to 0.237 mg.L-1, nitric 5-53 mg.L-1, DO ranged from 5.4 to 8.1 mg . L-1, pH values from 7.1 to 8.4, and temperature values from 23.2 to 25.8 0C. Water quality parameter values obtained relative stable during the culture period, and within the tolerance range for prawns (Table 1).

The results from second experiment showed that the optimum culture performance was obtained in the treatment II which was obtained highest biomass, SGR, bodyweight and body length of the shrimp with (1.96 + 0.05) g.cage-1, 2.40 %BW.day-1, (2.18 + 0,89) g and (6.50 + 0.91) cm, respectively. Even though the shrimp growth and performance in treatment II is higher than treatment I there is no significance different between treatments. The survival rate shown that there was significant different between the two treatments and control with 78% (control~treatment I), 92% (treatment II) and 90% (treatment III). During the experiments, the increase of nitrate concentration and ammonium can be controlled and maintained by addition of nitrifying bacteria and microalgae which can keep balance the microbial loop between ammonium reduction by bacteria and nitrate uptake by microalgae. Thus, addition of higher volume cubical cages results in higher shrimp growth and performance and addition of nitrifying bacteria and microalgae can maintain water quality with increases of stocking density up to 100%.

From the third experiment, the ponds with 20% shelter have

more significant effect compared to other treatment on growth and survival rate. Total prawn biomass with 20% shelter was 2485.02 ± 1724,84 gram, it was significantly difference compared to control and 40% shelter treatment with total biomass of 425,94 ± 394,14 gram and 238,32 ± 220,76 gram, respectively (P<0.05). The same trend can be seen in the survival rate, in which in control, 20% shelter treatment and 40% shelter treatment, the SR of 47,29 ± 33,86 %, 77,83 ± 11,98 % and 16,83 ± 14,4 %, respectively can be obtained. Survival rate in 20% shelter treatment was significantly difference compared to other treatment (P<0,05). From microbiological point of view, there are 10 species that was isolated from the shelter periphyton (20% treatment and 40% treatment) and the counts of these two treatments was remaining stable at 104 – 105 CFU.ml-1 until the end of the culture period. It could be stated that the periphytic biofiltration technology attached in 3-dimensional cubical bamboo shelter potentially can be used for the prawn nursery phase.

From the third experiment, the ponds with 20% shelter have more significant effect compared to other treatment on growth and survival rate. Total prawn biomass with 20% shelter was 2485.02 ± 1724,84 gram, it was significantly difference compared to control and 40% shelter treatment with total biomass of 425,94 ± 394,14 gram and 238,32 ± 220,76 gram, respectively (P<0.05). The same trend can be seen in the survival rate, in which in control, 20% shelter treatment and 40% shelter treatment, the SR of 47,29 ± 33,86 %, 77,83 ± 11,98 % and 16,83 ± 14,4 %, respectively can be obtained. Survival rate in 20% shelter treatment was significantly difference compared to other treatment (P<0,05). From microbiological point of view, there are 10 species that was isolated from the shelter periphyton (20% treatment and 40% treatment) and the counts of these two treatments was remaining stable at 104 – 105 CFU.ml-1 until the end of the culture period. It could be stated that the periphytic biofiltration technology attached in 3-dimensional cubical bamboo shelter potentially can be used for the prawn nursery phase.

Dr. Gede Suantika



1. 39The fourth experiment showed that the optimum culture performance was obtained on the culture with initial stocking density of 30 individuals.m-2 with final metabolic body weight, length, SGR, SR, FCR of (11.37 ± 4.92) gr, (10.69 ± 1.45) cm, 2.569%.day-1, 78.3%, and 0.99 respectively. However, from economic point of view, the use of 70 individuals.m-2 contributed to the highest total final biomass (975 gr) and biofiltration technology in zero-water discharge rearing system with the application of nitrifying bacteria can potentially increase the juvenile stocking density as well as maintain a good water quality to support a better growth and larval survival rate and profit of freshwater giant prawn M. rosenbergii industry.

It could be concluded that the addition of nitrifying bacteria and Chlorella sp as periphyton biofiltration agents combined with the use of 3-dimensional cubical bamboo as a shelter and attachment substrate of periphytic community enable the rearing system improve the culture performance (specific growth rate, survival rate, total biomass) as well as maintaining an acceptable ambient water quality parameter during the rearing period of the Fresh Water Giant Prawn (Macrobrachium rosenbergii de Man). Furthermore, the use of the developed technology contributes to a more promising profit since more optimum rearing condition can be maintained and the rearing system able to support higher prawn productivity per m2.

This review was made based on the followings publications:Suantika G., Astuti D.I., Arief R.R., Rusni M., Turendro O.R. (2012) “Use of Zero Water Discharge Technology through the Application of Nitrifying Bacteria and Textile Vertical Substrate in Grow-Out Phase of Macrobrachium rosenbergii De Man”. J. Aquacult. Res. Dev. 3:139 doi: 10.4172/2155-9546. 1000139.

Suantika G., Aditiawati P., Rusni M., Arief R.R., Turendro O.R. (2012). The Use of Biofiltration Technology and 3-Dimensional Cubical Bamboo Shelter for Nursery

Phase Productivity Improvement of Giant Freshwater Prawn. ITB Journal of Science Vol. 44 A No.2 p. 129-144.

Suantika, G., Astuti, D.I., Aditiawati, P., dan Sasmita, P.G. (2009). World Aquaculture 2009. Oral presentation: “Development of Zero-Water Discharge Technology and Nitrifying Bacteria Application in the Nursery Phase of Giant Freshwater Prawn (Macrobrachium rosenbergii de Man)”. Mexico, May 26 – 29.

Dr. Gede Suantika



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Table 1. Culture perforance of the first experiment Treatment

0 Shelter 2 Selter 4 Shelter 8 Shelter

Final Weight (gr) 1,007 ± 0,021 a 1,057 ± 0,184 a 0,999 ± 0,039 a 1,096 ± 0,054 a

Biomass (gr) 38,77 ± 0,716 a 50,48 ± 4,194 ab 63,17 ± 4,227 b 67,94 ± 1,605 b

Survival (%) 100.00 82.22 98.89 94.44

Ammonium (ppt) 0,060 ± 0,022 a 0,078 ± 0,057 a 0,096 ± 0,063 a 0,090 ± 0,068 a

Nitrite (ppt) 0,053 ± 0,005 a 0,062 ± 0,067 a 0,068 ± 0,069 a 0,035 ± 0,030 a

Nitrate (ppt) 34,238 ± 11,247 b 29,271 ± 11,878 ab 25,165 ± 10,045 a 26,261 ± 11,592 ab

Temperature (0C) 25,120 ± 0,550 a 25,125 ± 0,543 a 25,170 ± 0,530 a 25,201 ± 0,602 a

DO (ppt) 7,297 ± 0,378 c 7,009 ± 0,502 b 6,879 ± 0,475 b 6,642 ± 0,557 a

Table 2. Culture performance of the third experimentIII Treatment

0 Shelter 2 Selter 4 Shelter

Final Weight (gr) 0.93±0.24a 1.68±0.519a 0.84±0.26a

Biomass (gr) 425.94 ± 394.15a 2485.03 ± 1724.84b 238.32 ± 220.77a

Survival (%) 47.296±33.865ab 77.833±11.980a 16.833±14.486b

Ammonium (ppt) 0,125 a 0,1275 a 0,135 a

Nitrite (ppt) 0,00 a 0,00 a 0,00375 a

Nitrate (ppt) 16,875 a 16,5625 a 18,02125 a

Temperature (0C) 29,01 a 29,66 a 29,56 a

DO (ppt) 7,54 a 7,02 a 6,49 a

Table 3. Culture performance of the fourth experiment

Parameters

Treatment (Individual.m-2)

30 40 50 60 70

Mean Body Weight (g) 11.37 ± 4.96 9.34 ± 3.82 10.80 ± 5.62 10.98 ± 5.86 11.46 ± 4.517

SGR (% per day) 2.569 1.393 2.105 1.916 1.893

Survival Rate (%) 78.3 76.3 70.0 70.0 67.1

Total Biomass (g) 534.48 532.57 734.06 922.3 974.37

Total Feed (g) 526.75 625.87 864.49 1080.5 1334.14

Feed Conversion Ratio (FCR) 0.99 1.18 1.18 1.17 1.37

Feed Cost/kg (Rp) 9860 11750 11780 11720 13690

Seed Cost (Rp) 15600 20800 26000 31200 36400

Total Income (Rp) 27391 32545.24 44953.48 56186.00 69375.28

Profit (Rp) 1931 -4,76 7173,48 13266 19285,28

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Dra. Tati Suryati Syamsudin SubaharSchool of Life Sciences & Technology; Institut Teknologi Bandung. Jl. Ganesa No. 10 Bandung, 40132. Indonesia.Phone +62 22 251 1575, 250 0258 Fax: +62 22 253 4107 [email protected]

DEVELOPMENT OF AQUACULTURE RESEARCH AT SCHOOL OFLIFE SCIENCES AND TECHNOLOGY BANDUNG INSTITUTE OF

TECHNOLOGY, WEST JAVA-INDONESIA

Recently, the contribution of aquaculture to the world fishery production is up to 40% of the total production. This condition becomes an opportunity for aquaculture to fulfil the requirement of food sector of fishery products. Indonesia is the big five-fishery producer of the world has very strategic position on the progress and continuity of the industry.

At ITB level, the policy to develop and conduct a research on food security is one of vision-mission ITB as a high education institution in Indonesia, which are in line with its duty on education, research, and community services. For this mission, School of life Sciences and Technology is one of the faculties that have direct responsibility for implementation.

One of the research fields that have been developed for this mission is aquaculture. The basic ideas on aquaculture research and development at SITH-ITB is how to create an innovative research and development on the rearing strategy that enable the system in producing high productivity of fish/shrimp, efficient in water, space, and feed use, zero waste, adaptable, and reliable.

For this, an integrated closed aquaculture system is the solution. For this closed technology, the development at SITH-ITB has been made by following several consecutive steps, including: (1) isolation of local microbe, (2) detail engineering design of the system, (3) optimization of the system either at laboratory or pilot scale, (4) application at industrial scale.

After we come with the proven closed system technology, we had conducted an integrated research for continual improvement of the closed system, such as (1) application of bioflocs technology, (2) application of probiotic, (3) vaccine development, and (4) feed development. Two closed aquaculture system that are developed for aquaculture purposes: (1) Recirculating aquaculture system and (2) Zero water discharge technology.

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By using these two systems, several aquaculture commodities including prawn, shrimp, tilapia, gourami, common carp, ornamental freshwater fish, etc. have been reared and evaluated. We try to analysis the industrial feasibility of the system at several phases in aquaculture industry, such as broodstock rearing, larviculture, nursery, as well as for grow-out.

The technologies also have been installing at several aquaculture activities site in Indonesia. By using this closed aquaculture system, we will be able to create more control culture condition, hygienic, more efficient of resources use, and more flexible so a sustainable aquaculture industry could be realized.

Dra. Tati Suryati Syamsudin Subahar

DEVELOPMENT OF AQUACULTURE RESEARCH AT SCHOOL OFLIFE SCIENCES AND TECHNOLOGY BANDUNG INSTITUTE OF

TECHNOLOGY, WEST JAVA-INDONESIA

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Dra. Jaqueline de Medeiros O. GastelúDirectora-Sócia, M. Sc. Aquicultura - UFSC. LARVI Aquicultura & Projetos LTDA. Natal - RN - Brasil [email protected]

EXPERIENCIAS CON LARVICULTURA DE Macrobrachium carcinus (CAMARÓN PITÚ) EN LARVI AQUICULTURA & PROJETOS LTDA,

SITUADA EM LA REGIÓN NORESTE DEL BRASIL

El desarrollo de investigaciones con la especie nativa Macrobrachium carcinus, conocida como Pitú, que en la región Noreste del Brasil es importante tanto desde el punto de vista comercial como ambiental, esta especie pertenece a la família Palaemonidae y está entre los camarones de agua dulce de mayor tamaño, además de presentar um alto índice de consumo mundial, teniendo una amplia distribución ocurriendo en águas dulces e salobres. En el Brasil su distribución es desde Pará hasta Rio Grande do Sul y es considerada una espécie nativa con gran potencial para la carcinicultura de agua dulce, apesar de que no se disponibiliza de muchas informaciones sobre los aspectos ecológicos y biológicos.

M. carcinus está en la lista de espécies amenazadas de extinción, en virtud de diferentes factores, como la sobrepesca, la polución industrial y la destrucción de ecosistemas naturales, que comprometen su sobrevivencia. Hay documentaciones que afirman que en el Brasil M. carcinus se viene estudiando desde 1956, y actualmente instituciones públicas como la Universidade Federal Rural de Pernambuco (UFRPE), la Universidade Federal do Rio Grande do Norte (UFRN) y la Universidade Federal de Alagoas (UFAL), mantienen estudios sobre esta espécie.

La empresa LARVI, ubicada en el estado de Rio Grande do Norte - Brasil, inició en el año 2009 el Proyecto “Desarrollo de tecnologias de larvicultura de el camarón nativo de água dulce Pitú y nuevos métodos para la larvicultura del camarón comercial del género Macrobrachium (Bate, 1868)”, contando inicialmente com el apoyo financiero de la FAPERN (Fundação de Apoio à Pesquisa do Estado do Rio Grande do Norte) y científico de la UFRN (Universidade Federal do Rio Grande do Norte). Entre los objetivos del proyecto, se destaca la producción de la espécie nativa Pitú para el repoblamiento dos azudes y reservórios del Estado.

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incrementar la sobrevivencia larval y contribuir con la producción y preservación de M. carcinus.

Queda sobre entendido que la utilización de reproductores selecionados, a partir de stocks cultivados en estanques, asi como, el dominio de la reproducción en laboratorio, será de fundamental importancia para el cultivo de esta especie en el futuro.

Se sabe que, todavia hay mucho por investigar en los procesos de larvicultura de M. carcinus, sobretodo en relación a las necesidades nutricionales específicas, al comportamiento en cultivo y el desarrollo larval. Todo esto, con la finalidad de optimizar la calidad de las post-larvas producidas.

Para viabilizar este proyecto, la LARVI adaptó y mejoró las técnicas de manejo para la producción de post-larvas do Pitú, utilizando su própria experiencia, asimilada con la larvicultura comercial de Macrobrachium rosenbergii, especie sobre la cual Brasil posee tecnologia de cultivo larval en diferentes sistemas de cultivo desde los años 80’s.

Inicialmente, fueron colectadas hembras ovígeras de la especie M. carcinus, en azudes y reservórios en el interior del estado de Rio Grande do Norte. En consecuencia la LARVI realizó el cultivo larval en tanques con volumen de 1000 litros, utilizando una dieta con alimentación diversificada y de buena calidad, compuesta de naúplios de Artemia, ración artesanal y raciones artificiales bioencapsuladas, comumente utilizadas para las larvas de camarón marino. Para manter la calidad da agua se utilizó probióticos y se realizó recambios de agua durante todo o ciclo.

Uno de los desafios fue encontrar la salinidad ideal para el cultivo, que después de las pruebas, apuntó 20 psu como la salinidad donde se obtuvieron las mejores sobrevivencias. Fueron realizadas diversas tentativas hasta consolidar la primera producción de póst-larvas en 2011, y que después continuó hasta 2013, cuando el proyecto fué finalizado.

Las post-larvas producidas en la larvicultura, fueron utilizadas para la formación de planteles de reproductores, repoblamientos de azudes y barragens, además de donaciones a Instituciones públicas e del gobierno, para la realización de investigaciones en la fase de pre-cria y engorda. Los trabajos desarrollados permitieron la obtención de hembras ovígeras en el propio laboratorio, posibilitando la producción de póst-larvas de forma constante.

Actualmente la LARVI continua desarrollando investigaciones sobre la larvicultura de esta especie, con el apoyo del Proyecto Aquiflora / ABDA, y produciendo post-larvas de Pitú en pequeña escala, además de colaborar con la formación de recursos humanos através de estancias y entrenamientos de personas provenientes de diferentes instituciones. La LARVI tiene como objetivo el apropiamiento constante de sus técnicas de cultivo para asi

Dra. Jaqueline de Medeiros O. Gastelú

EXPERIENCIAS CON LARVICULTURA DE Macrobrachium carcinus (CAMARÓN PITÚ) EN LARVI AQUICULTURA & PROJETOS LTDA,

SITUADA EM LA REGIÓN NORESTE DEL BRASIL

28 al 30 de mayo de 2014 villahermosa tabasco; méxico · m. en a. rubicel cruz romero secretario...

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