ATGGCGGCGGCGGCGGCGGCGGGCGCGGGCCCGGAGATGGTCCGCGGGCAGGTGTTCGACGTGGGGCCGCGCTACACCAACCTCTCGTACATCGGCGAGGGCGCCTACGGCATGGTGTGCTCTGCTTATGATAATGTCAACAAAGTTCGAGTAGCTATCAAGAAAATCAGCCCCTTTGAGCACCAGACCTACTGCCAGAGAACCCTGAGGGAGATAAAAATCTTACTGCGCTTCAGACATGAGAACATCATTGGAATCAATGACATTATTCGAGCACCAACCATCGAGCAAATGAAAGATGTATATATAGTACAGGACCTCATGGAAACAGATCTTTACAAGCTCTTGAAGACACAACACCTCAGCAATGACCATATCTGCTATTTTCTCTACCAGATCCTCAGAGGGTTAAAATATATCCATTCAGCTAACGTTCTGCACCGTGACCTCAAGCCTTCCAACCTGCTGCTCAACACCACCTGTGATCTCAAG:::GCCCTGAGCCTGTGCAATTATTTCGAGAGTCAAAATGTGGATTTCCGAGGCAAGAAGGTGATCGAACTGGGTGCGGGGACAGGCATCGTGGGGATCTTGGCAGCGCTGCAGGGGGGGGATGTTACCATCACTGACCTGCCCCTGGCCCTAGAACAGATCCAGGGCAACGTCCAGGCCAATGTGCCAGCTGGAGGCCAGGCCCAGGTGCGTGCCTTGTCCTGGGGGATTGACCATCATGTCTTCCCTGCAAACTATGACCTGGTGCTGGGGGCTGATATCGTGTACCTGGAACCCACCTTCCCTCTGCTGCTGGGGACCCTCCAACACCTGTGCAGGCCCCATGGCACCATCTATCTGGCCTCCAAGATGAGAAAGGAGCATGGGACAGAGAGCTTCTTTCAGCACCTCCTGCCCCAGCATTTCCAACTGGAGCTGGCTCAGCGGGATGAGGATGAAAATGTCAACATCTATAGGGCCAGGCACAGGGAACCAAGACCTGCTTGA

MAPK1/METTL21B fusionstrand+ strand-

1Kb

Ladd

er33

16

3296

3338

3304

C-

850bp

500bp

1000bp

300bp400bp

200bp

100bp

650bp534bp

EX3/EX2

1Kb

Ladd

er33

1632

96

3338

3304

C-

850bp

650bp

1000bp

400bp

500bp

300bp

200bp

659bp

CAND1/EGFR fusion

strand+ strand-

ATGGCGAGCGCCTCGTACCACATTTCCAATTTGCTGGAAAAAATGACATCCAGCGACAAGGACTTTAGGTTTATGGCTACAAATGATTTGATGACGGAACTGCAGAAAGATTCCATCAAGTTGGATGATGATAGTGAAAGGAAAGTAGTGAAAATGATTTTGAAGTTATTGGAAGATAAAAATGGAGAGGTACAGAATTTAGCTGTCAAATGTCTTGGTCCTTTAGTGAGTAAAGTGAAAGAATACCAAGTAGAGACAATTGTAGATACCCTCTGCACTAACATGCTTTCTGATAAAGAACAACTTCGAGACATTTCAAGTATTGGTCTTAAAACAGTAATTGGAGAACTTCCTCCAGCTTCCAGTGGCTCTGCATTAGCTGCTAATGTATGTAAAAAGATTACTGGACGTCTTACAAGTGCAATAGCAAAACAGGAAGATGTCTCTGTTCAGCTAGAAGCCTTGGATATTATGGCTGATATGTTGAGCAG:::ATGCACTGGGCCAGGTCTTGAAGGCTGTCCAACGAATGGGCCTAAGATCCCGTCCATCGCCACTGGGATGGTGGGGGCCCTCCTCTTGCTGCTGGTGGTGGCCCTGGGGATCGGCCTCTTCATGCGAAGGCGCCACATCGTTCGGAAGCGCACGCTGCGGAGGCTGCTGCAGGAGAGGGAGCTTGTGGAGCCTCTTACACCCAGTGGAGAAGCTCCCAACCAAGCTCTCTTGAGGATCTTGAAGGAAACTGAATTCAAAAAGATCAAAGTGCTGGGCTCCGGTGCGTTCGGCACGGTGTATAAGGGACTCTGGATCCCAGAAGGTGAGAAAGTTAAAATTCCCGTCGCTATCAAGGAATTAAGAGAAGCAACATCTCCGAAAGCCAACAAGGAAATCCTCGATGAAGCCTACGTGATGGCCAGCGTGGACAACCCCCACGTGTGCCGCCTGCTGGGCATCTGCCTCACCTCCACCGTGCAGCTCATCACGCAGCTCATGCCCTTCGGCTGCCTCCTGGACTATGTCCGGGAACACAAAGACAATATTGGCTCCCAGTACCTGCTCAACTGGTGTGTGCAGATCGCAAAGGGCATGAACTACTTGGAGGACCGTCGCTTGGTGCACCGCGACCTGGCAGCCAGGAACGTACTGGTGAAAACACCGCAGCATGTCAAGATCACAGATTTTGGGCTGGCCAAACTGCTGGGTGCGGAAGAGAAAGAATACCATGCAGAAGGAGGCAAAGTGCCTATCAAGTGGATGGCATTGGAATCAATTTTACACAGAATCTATACCCACCAGAGTGATGTCTGGAGCTACGGGGTGACTGTTTGGGAGTTGATGACCTTTGGATCCAAGCCATATGACGGAATCCCTGCCAGCGAGATCTCCTCCATCCTGGAGAAAGGAGAACGCCTCCCTCAGCCACCCATATGTACCATCGATGTCTACATGATCATGGTCAAGTGCTGGATGATAGACGCAGATAGTCGCCCAAAGTTCCGTGAGTTGATCATCGAATTCTCCAAAATGGCCCGAGACCCCCAGCGCTACCTTGTCATTCAGGGGGATGAAAGAATGCATTTGCCAAGTCCTACAGACTCCAACTTCTACCGTGCCCTGATGGATGAAGAAGACATGGACGACGTGGTGGATGCCGACGAGTACCTCATCCCACAGCAGGGCTTCTTCAGCAGCCCCTCCACGTCACGGACTCCCCTCCTGAGCTCTCTGAGTGCAACCAGCAACAATTCCACCGTGGCTTGCATTGATAGAAATGGGCTGCAAAGCTGTCCCATCAAGGAAGACAGCTTCTTGCAGCGATACAGCTCAGACCCCACAGGCGCCTTGACTGAGGACAGCATAGACGACACCTTCCTCCCAGTGCCTGAATACATAAACCAGTCCGTTCCCAAAAGGCCCGCTGGCTCTGTGCAGAATCCTGTCTATCACAATCAGCCTCTGAACCCCGCGCCCAGCAGAGACCCACACTACCAGGACCCCCACAGCACTGCAGTGGGCAACCCCGAGTATCTCAACACTGTCCAGCCCACCTGTGTCAACAGCACATTCGACAGCCCTGCCCACTGGGCCCAGAAAGGCAGCCACCAAATTAGCCTGGACAACCCTGACTACCAGCAGGACTTCTTTCCCAAGGAAGCCAAGCCAAATGGCATCTTTAAGGGCTCCACAGCTGAAAATGCAGAATACCTAAGGGTCGCGCCACAAAGCAGTGAATTTATTGGAGCATGA

EX4/EX16

ATGAGGCGCCAGCCTGCGAAGGTGGCGGCGCTGCTGCTCGGGCTGCTCTTGGAG:::CATATTGAAGGTGATGACCTGCATATCCAGAGGAATGTGCAAAAGCTGAAGGACACAGTGAAAAAGCTTGGAGAGAGTGGAGAGATCAAAGCAATTGGAGAACTGGATTTGCTGTTTATGTCTCTGAGAAATGCCTGCATTTGA

1Kb

Ladd

er33

1632

96

3338

3304

C-

850bp650bp

1000bp

400bp500bp

300bp

200bp

100bp122bp

VOPP1/IL22 fusionEX1/EX4

ATGTGGCTGAAGGTGGGGGGCCTACTTCGGGGGACCGGTGGACAGCTGGGCCAGACTGTTGGTTGGCCTTGTGGGGCCCTGGGGCCTGGGCCCCACCGCTGGGGACCATGTGGAGGTTCTTGGGCCCAAAAGTTTTACCAGGATGGGCCTGGGAGAGGCCTGGGTGAGGAGGACATTCGCAGGGCACGGGAGGCCCGTCCCAGGAAGACACCCCGGCCCCAGCTGAGTGACCGCTCTCGAGAACGCAAGGTGCCTGCCTCCCGCATCAGCCGCTTGGCCAACTTTGGGGGACTGGCTGTGGGCTTGGGGCTAGGAGTACTGGCCGAGATGGCTAAGAAGTCCATGCCAGGAGGTCGTCTGCAGTCAGACAACAGCTTCATCAGCCCTCAGCTGCAGCACATCTTTGAGCGGGTCCGCCAGAGCGCCGACTTCATGCCCCGCTGGCAGATGCTGAGAGTTCTTGAAGAGGAGCTCGGCAGGGACTGGCAGGCCAAGGTGGCCTCCTTGGAGGAGGTGCCCTTTGCCGCTGCCTCAATTGGGCAGGTGCACCAGGGCCTGCTGAGGGACGGGACGGAGGTGGCCGTGAAGATCCAGTACCCCGGCATAGCCCAGAGCATTCAGAGCGATGTCCAGAACCTGCTGGCGGTACTCAAGATGAGCGCGGCCCTGCCCGCGGGCCTGTTTGCCGAGCAGAGCCTGCAGGCCTTGCAGCAGGAGCTGGCTTGGGAGTGTGACTACCGTCGTGAGGCGGCTTGTGCCCAGAATTTCAGGCAGCTGCTGGCAAATGACCCCTTCTTCCGGGTCCCAGCCGTGGTTAAGGAGCTGTGCACGACACGGGTGCTGGGCATGGAGCTGGCTGGAGGGGTCCCCCTGGACCAGTGCCAGGGCCTAAGCCAGGACCTGCGGAACCAGATTTGCTTCCAGCTCCTGACGCTGTGTCTGCGGGAGCTGTTTGAGTTCCGATTCATGCAGACTGACCCCAACTGGGCCAACTTCCTGTATGATGCCTCCAGCCACCAGGTGACCCTGCTGGACTTTGGTGCAAGCCGGGAGTTTGGGACAGAGTTCACAGACCATTACATCGAGGTGGTGAAGGCTGCAGCTGATGGAGACAGAGACTGTGTCCTGCAGAAGTCCAGGGACCTCAAATTCCTCACAGGCTTTGAAACCAAGG:::GCGGACCCCGGAGGCCTGAGCGGCACCTGCCCCCAGCCCCCTGTGGGGCCCCGGGGCCCCCAGAAACCTGCAGGACGGAGCCAGACGGGGCGGGCACCATGAACAAGTTACGGCAGAGCCTGCGGCGGAGGAAGCCAGCCTACGTGCCCGAGGCGTCGCGCCCGCACCAGTGGCAGGCAGACGAGGACGCGGTGCGGAAGGGCACGTGCAGCTTCCCGGTCAGGTACCTGGGTCACGTGGAGGTAGAGGAGTCCCGGGGAATGCACGTGTGTGAAGATGCGGTGAAGAAGCTGAAGGCGATGGGCCGAAAGTCCGTGAAGTCTGTCCTGTGGGTGTCAGCCGATGGGCTCCGAGTGGTGGACGACAAAACCAAGGATCTTCTGGTCGACCAGACCATCGAAAAGGTCTCCTTTTGTGCTCCTGACCGCAACCTGGACAAGGCTTTCTCCTATATCTGTCGTGACGGGACTACCCGCCGCTGGATCTGCCACTGTTTTCTGGCACTGAAGGACTCCGGCGAGAGGCTGAGCCACGCTGTGGGCTGTGCTTTTGCCGCCTGCCTGGAGCGAAAACAGCGACGGGAGAAGGAATGTGGGGTCACGGCCGCCTTCGATGCCAGCCGCACCAGCTTCGCCCGCGAGGGCTCCTTCCGCCTGTCTGGGGGTGGGCGGCCTGCTGAGCGAGAGGCCCCGGACAAGAAGAAAGCAGAGGCAGCAGCTGCCCCCACTGTGGCTCCTGGCCCTGCCCAGCCTGGGCACGTGTCCCCGACACCAGCCACCACATCCCCTGGTGAGAAGGGTGAGGCAGGCACCCCTGTGGCTGCAGGCACCACTGCGGCCGCCATCCCCCGGCGCCATGCACCCCTGGAGCAGCTGGTTCGCCAGGGCTCCTTCCGTGGGTTCCCAGCACTCAGCCAGAAGAACTCGCCTTTCAAACGGCAGCTGAGCCTACGGCTGAATGAGCTGCCATCCACGCTGCAGCGCCGCACTGACTTCCAGGTGAAGGGCACAGTGCCTGAGATGGAGCCTCCTGGTGCCGGCGACAGTGACAGCATCAACGCTCTGTGCACACAGATCAGTTCATCTTTTGCCAGTGCTGGAGCGCCAGCACCAGGGCCACCACCTGCCACAACAGGGACTTCTGCCTGGGGTGAGCCCTCCGTGCCCCCTGCAGCTGCCTTCCAGCCTGGGCACAAGCGGACACCTTCAGAGGCTGAGCGATGGCTGGAGGAGGTGTCACAGGTGGCCAAGGCCCAGCAGCAGCAGCAGCAGCAACAGCAACAGCAGCAGCAGCAGCAGCAGCAACAGCAGCAAGCAGCCTCAGTGGCCCCAGTGCCCACCATGCCTCCTGCCCTGCAGCCTTTCCCCGCCCCCGTGGGGCCCTTTGACGCTGCACCTGCCCAAGTGGCCGTGTTCCTGCCACCCCCACACATGCAGCCCCCTTTTGTGCCCGCCTACCCGGGCTTGGGCTACCCACCGATGCCCCGGGTGCCCGTGGTGGGCATCACACCCTCACAGATGGTGGCAAACGCCTTCTGCTCAGCCGCCCAGCTCCAGCCTCAGCCTGCCACTCTGCTTGGGAAAGCTGGGGCCTTCCCGCCCCCTGCCATACCCAGTGCCCCTGGGAGCCAGGCCCGCCCTCGCCCCAATGGGGCCCCCTGGCCCCCTGAGCCAGCGCCTGCCCCAGCTCCAGAGTTGGACCCCTTTGAGGCCCAGTGGGCGGCATTAGAAGGCAAAGCCACTGTAGAGAAACCCTCCAACCCCTTTTCTGGCGACCTGCAAAAGACATTCGAGATTGAACTGTAG

ADCK4/NUMBL fusion

1Kb

Ladd

er33

1632

9633

3833

04C

-

850bp

650bp

1000bp

400bp500bp

300bp

200bp

100bp

313bp

EX14/EX2