fisologÍa-de-las-levaduras-1751-1-1.pptx

7/26/2019 FISOLOGA-DE-LAS-LEVADURAS-1751-1-1.pptx

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FISOLOGA DELAS LEVADURAS

LABORATORIO DE MICROBIOLOGIA GENERAL II

SEMESTRE: 2016-1 GRUPO: 1751 EQUIPO: 11

INTEGRANTES:Corona Rodr!"#$ %"&'#(a I)#&*&or#+ R#,#+ Br'+'aG'& Oro$o ./(orQ"#o& Rar#$ G#rardo


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OBJETIVOS

Cono#r &a+ n##+'dad#+ +'o&3!'a+ d# &a+ad"ra+

Cor#nd#r , r#a&'$ar &a+ (/n'a+ d#A")ono!raa , #& 'o!raa8 ara &a'd#n('a'3n d# ad"ra+

Cono#r &a 'or(an'a &n'a # 'nd"+(r'a& d#'d#n('ar ad"ra+


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.on!o+

.on!o+

&a#n(o+o+ L#4ad"ra+


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LEVADURAS

En('dad#+ M'ro9'o&3!'a+'nd##nd'#n(#+

Un'#&"&ar#+ Con #9rana

Par#d #&"&ar a+' +'#r# d#"'('na

C'(o&a+a on 4a"o&a+ ,n;o


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La &"r'#&"&ar'dd +# r#+#n(a "ando:

*ora #+(r"("ra+ #&a9orada+ oo+#"do'#&'o


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FORMA

G&o9o+a+

O4o'd#+

E&on!ada+ R#(an!"&ar#+

C'&ndr'a+

Tr'an!"&ar#+


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REQUERIMIENTOS FISICOS DECRECIMIENTO

Ran!o d# . 2> >5

Optimo 4.5 6.5

T##ra("ra d# r#''#n(o d# O a ?7@C

Optima 25C

N##+'(an a!"a ara +" r#''#n(o


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REQUERIMINETOS NUTRICIONALES

N##+'(an "#n(#+ d# ar9ono


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ASIMILACIN Y FERMENTACION DECARBOHIDRATOS

Asimilaci: 4a&oran &a aa'dad d# &a ad"raara #(a9o&'$ar #n ond''on#+ a#ro9'a+8

a$;ar#+8 a&oo+ , 'do+ or!n'o+


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$IMOGRAMA

S# r#a&'$a #n #d'o d# "&('4o &'"'do on or'3n d#ar9ono #n(r# 1-5

S# a!r#!a "n 'nd'ador d# .


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AU-ONOGRAMA

M#d'o +o&'do 9a+# #(onado ar#n(# d#

ar9o'dra(o+ +# +'#9ra

Po+(#r'or#n(# +# a!r#!ara #&Car9o'dra(o a 'n4#+('!ar #n ora d##n''&'ndro+ o d'+o+ d# a#& &(ro

'r#!nado+ on #& +"+(ra(o8 on "naon#n(ra'3n d#& 1-2

S# 'n"9a a 25oC d# 5-15 da+

E& d#+arro&&o d# &a o&on'a a&r#d#dor d# &o+ar9o'dra(o+ 'nd'an


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%RUEBA DE LA UREASA

E& aon'ao o(or!a ond''on#+a&a&'na+ a& #d'o , a9'a #&'nd'ador


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ALTERNATIVA5 Ino"&ar &a +"#r'# d# "n ("9o #n 'o d# a"(a d#a!ar-"r#a d# Cr'+(#n+#n

PRUEBA DE UREASARJPIDA


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Pa+ar &a "n(a d# "na&'ador d# a&!od3n

'r#!nado on

a!ar-"r#a d#Cr'+(#n+#nd#+'dra(ado +o9r#

&a +"#r'# d# 2 o o&on'a+ a'+&ada+ d#&

on!o

INOCULACIKN

INCUBACIKN

E)a'nar #& ("9od#+"/+ d# 108 158

20 , 0 'n E&

d#+arro&&o d# "no&or roo ro+ado'nd'a "# #&r#+"&(ado #+

o+'('4o

INTERPRETACIKN

Co&oar #& a&'ador'no"&ado #n "n ("9oon !o(a+ d# &or"ro

d# 9#n$a&on'o 1 < .?>6 001=, ro(ar #n#& ondo d#& ("9o ara

d#o+'(ar #& on!oTaar #& ("9o # 'no"&ar

a ?5@C

PRUEBA DE UREASA RJPIDASELECTIA


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REDUCCIN DE NITRATOS

('& #n &a d'#r#n'a'3n d# #+#'#+d# Cr'(ooo+

Po+'('4o: E& d#+arro&&o 'n#d'a(o d# "no&or ro+a a roo 'n(#n+o

N#!a('4o: Aar'&&o

Ca/aci+a+ +! &a l!0a+&"a +! "!+&ci" i#"a#(s ai#"i#(s

Es/!ci!s +!Cryptococcus

6NO7

C. neoformans -

C. Uniguttulatus -

C. gastricus -C. laurentii -

C. luteolus -

C. lbidus !ar. di"uens

C. lbidus !ar. albidus

C. terreus


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E& a&'ador'no"&ado +# o&oa

#n "n ("9o , +#r#+'ona on r#$a#n #& ondo d#& ("9o

E& ("9o , #& '+oo+# 'n"9an d"ran(#10 'n"(o+ a ?5@C

PRUEBA RJPIDA DE REDUCCION DENITRATO


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MEDIO AGAR HARINA DE MAI$

E& o&'+or9a(o >0 r#d"# &a (#n+'3n +"#r'a& d#d'o roo4'#ndo &a rod"'3n d#&a'doon'd'o+ , +#"do'a+

S# "('&'$a ara &a d#(#'3n d# &a'do+ora+rod"'da+ or C. albicans

Para &a d'#r#n'a'3n d# &o+ !/n#ro+ Cryptococcus#$acc%aromyces# &eotric%um y 'ric%osporon.

C(m/(sici

.ar'na d# a'$ 2 !

A!ar 15 !

Po&'+or9a(o >0 0= 15 &

A!"a d#+('&ada 1000 &


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%RODUCCIN DE CLAMIDOS%ORAS

Son ora+ d# r#+'+(#n'a8 r#donda+ " o4a+8 d# 6-12 d# d'#(ro , ar#d !r"#+a8 on a+#(o d# #+ora+&a(#ra+ o (#r'na+

Candidaalbicans

Candidadubliniensis


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TUBO GERMINAL

E+ "na r"#9a ;('& arad'#r#n'ar C.albicansC.dubliniensisd# Cndida NO albicans.

S# r#a&'$a a ar('r d# "na o&on'ar#+a d# r#''#n(o="('&'$ando &a+a r#+o o +"#ro"ano on !&"o+a 'n"9ando a7 @C or )'o ora+


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E& ("9o !#r'na& #+ "na#)(#n+'3n &a#n(o+a d# &aad"ra8 +'n #+(r#a'#n(o


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Para on+'d#rar "na r"#9a

oo o+'('4a d#9#n o9+#r4ar+#+ d# 5 ("9o+ !#r'na+

E+(a #+ r"#9a r#+"n('4a ,a"# no (odo+ &o+ a'+&a'#n(o+d# C. albicans +on ("9o!#r'na& o+'('4o , (a9'/n +#

r#+#n(an a&+o+ n#!a('4o+


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C.ROMa!ar

*or"&a or &'(ro d# a!"a

Croo#(ona 1080!

G&"o+a 2080

M#$&a

ro3!#na

280

C&oran#9'o& 085

A!ar 1580

. 680 08

Los agares cromognicos son

medios selectivos y diferenciales

disponibles comercialmente.

Contiene una mezcla de sustratos

cromgenos que liberan unoscomponentes coloreados diferentes

cuando son degradados por

enzimas especficas producidas por

ciertas especies de Candida.


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E+(# #d'o #r'(# #& d#+arro&&o d# &a+ #+#'#+ +o"n#+ d# Candida , on!o+ ad"r'or#+ #d'an(#

&a ora'3n d# o&on'a+ o&or'da+ d'#r#n'ada+: C. albicans


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CHROMOAGAR

S'r4# oo #d'o d# "&('4o+r'ar'o+

Una d# &a+ r'n'a+

4#n(aa+ d# #+(o+ #d'o+ #+#r'('r d'#r#n'ar'n(# &o+ "&('4o+')(o+

P#r'(# 'd#n('ar &a+

#+#'#+ &n'a#n(#'or(an(#+ d#& !/n#roCandida .

Es/!ci! T&'( 3!"mial U"!a CHROMa3a"8


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Es/!ci! T&'( 3!"mial U"!a CHROMa3a"8

C. albicans - #rd# &aro

C. glabrata - - 'o(a 'n(#n+o

C. guillermondii - - 'o(a &'do

C. krusei - - 'o(a &'do

C. parapsilosis - - B&ano-r#a

C. tropicalis - - A$"& o+"ro

C. dubliniensis #rd# o+"ro

Rhodotorula spp. - No a&'a

Saccharomyces spp. - - No a&'a

Trichosporon spp. - A$"&-!r'+

Geotrichum spp. - - 'o(a


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CRITERIOS PARA IDENTI*ICACIKN

Maro+3'o+


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%RUEBAS COMERCIALES %ARALEVADURAS

S'+(#a Un'-#a+(-T#H

API 20C AU

API-EAST-IDENT

ID 2 C


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A%I 9:C AU-8

S# oon# d# 20 ;"&a+ on +"+(ra(o+ d#+'dra(ado+ "# #r'(#nr#a&'$ar 1 r"#9a+ d# a+''&a'3n

S# 'no"&an on "n #d'o n'o +#'+3&'do8 &a+ ad"ra+ +3&o +#r#rod"#n +' +on aa#+ d# "('&'$ar #& +"+(ra(o orr#+ond'#n(#

P#r'(# 'd#n('ar "n (o(a& d# ? #+#'#+ d'#r#n(#+


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METODOLO

GI

A


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PRODUCCIKN DE CLAMIDIOESPORAS

D'4'd'r #d'o a!ar ar'na d# a$ , +#9rar

#n ora d#

In"9ar a 7C or 2? a ?> ora+

O9+#r4ar a& 'ro+o'o a!r#!ando "na !o(ad# +o&"'3n +a&'na


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PRODUCCIKN DE TUBO GERMINAL

En 2 ("9o+ d# #n+a,# o&oar

aro)'ada#n(# 1L d# +"#ro d#on#o8 arn#ro o "ano

Ino"&ar a+ada d# #a d# on!o #n("9o on +"#ro # 'n"9ar a 5@C or

Co&oar "na !o(a #n or(ao9#(o+8"9r'r , o9+#r4ar a& 'ro+o'o


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AUONOGRAMA

S"+#nd#r &a ad"ra #n +o&"'3n +a&'na

#+(/r'&

S#9rar a+'4a#n(# ada ad"ra #n +"aa orr#+ond'#n(#

."#d##r &o+ d'+o+ on V" d# &a++o&"'on#+ d# &o+ ar9o'dra(o+8 +#ar #)#+o , o&oar &o+ d'+o+ #n &a+ aa+ d#P#(r'

In"9ar a 7@C or 2?-?>

R#or(ar #& r#''#n(o


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IMOGRAMA

Ino"&ar ad"ra #n ada ("9o onar9o'dra(o , "r"ra d# 9roor#+o 'n"9ar

In"9ar a 7@C or 2?-?>

R#or(ar +' a, #r#n(a'3n


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BIBLIOGRA*WA

Xon#an E M'o&o!a: Pr('a d# La9ora(or'o Y #d''3n8 Ed'(or'a& Pana#r'anaB"#no+ A'r#+ 1>7 P 175-11

Ba,, and So((Z+ D'a!n3+('o 'ro9'o&3!'o 12Y #d''3n Ed'(or'a& M/d'aana#r'ana Ar!#n('na 200

Bon'a$ A M'o&o!a M/d'a B+'a M#nd#$ #d'(or#+ M/)'o8 1?

Ar#na+ G"$n Ro9#r(o M'o&o!a M/d'a '&"+(rada 2da #d Ed'(or'a& M Gra .'&&M/)'o 200


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fisologÍa-de-las-levaduras-1751-1-1.pptx

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