PPA una enfermedad semi-ignorada

No existe una vacuna frente a la PPA

- Utilización de virus inactivados no confiere protección

- Utilización de virus atenuados elevada protección reversión a virulento

animales portadores

PESTE PORCINA AFRICANA (GAN)

2005-2007 AGL2004-07857-C03-012008-2010 AGL2007-66441-C03-01/GAN

2011-2013 AGL2010-22229-C0

2014-2016 AGL2013-48998-C2-1-R

COORDINACION

Divulgación: ARCS2009 y ARCS2012-100331

INIA: AC2012-00074-00-00

Cooperación Int: PCI2005-A7-0092 con Turquía y Acción

Integrada Hispano-Francesa (HF-2005-0204)

Movilidad: Paula López-AYUDA PREDOCTORAL 2014 EEBB-I-14-08298Fernando Rodríguez-Beca Salvador Madariaga 2013

Empresa: INIA-TRT2006-00035-C02-01

UE: 2009 EPIZONE Net (EU).

Consolider Ingenio CSD2006-7. 2320

ESTRATEGIAS DE PROTECCION FRENTE A LA PESTE

PORCINA AFRICANA: DE LA INVESTIGACION BASICA

AL PROTOTIPO VACUNAL

AGL2013-48998-C2-1-R 2014-2016

COORDINACION

CReSA CBM

F Rodriguez& F Accensi

Boehringer Ingelheimm

A BensaidS PinaBSL-2BSL-3

A Gallei

G Andrés& ML Salas

Lo que consideramos imprescindible para pedir un proyecto

o Coordinación con grupos que lo necesiten tanto comonosotros Coordinarse no debería ser simplemente “juntarse” Han de existir (o crear) objetivos comunes Cada grupo debe tener adicionalmente sus propios

objetivoso Basar el proyecto principalmente en resultados propios

POR NUESTRA PARTE

POR PARTE DE LA ADMINISTRACIONo Flexibilidad técnica (justificaciones…) o Flexibilidad administrativa evitar una “fiscalización excesiva” Confianza en el investigador

PROTOTIPO VACUNAL

AGL2013-48998-C2-1-R 2014-2016

CONVENIO y ACUERD0

CSIC-BI-CReSA (IRTA)• Patente• Contratos experimento a experimento

2009 Jordi Marqués Argilaguet. Desarrollo de nuevas

estrategias vacunales frente al virus de la peste porcina

africana (VPPA). Calificación Máxima de Sobresaliente Cum

Laude. Universitat Autónoma de Barcelona (UAB).

Beca FPI asociada al proyecto AGL2004-07857-

C03-01; Investigador Postdoctoral en la UPF

(PRBB-Barcelona)

2013 Argilaguet JM, Pérez-Martín E, López S, Goethe M, Escribano JM,

Giesow K, Keil GM, Rodríguez F. BacMam immunization partially protects

pigs against sublethal challenge with African swine fever virus. Antiviral Res.

Feb 18;98(1):61-65.

2012 Argilaguet JM, Pérez-Martín E, Nofrarías M, Gallardo C, Accensi F,

Lacasta A, Mora M, Ballester M, Galindo-Cardiel I, López-Soria S, Escribano

JM, Reche PA, Rodríguez F. DNA vaccination partially protects against

African swine fever virus lethal challenge in the absence of antibodies. PLoS

One. 7(9):e40942. doi: 10.1371/journal.pone.0040942.

2011 Argilaguet JM, Pérez-Martín E, Gallardo C, Salguero FJ, Borrego B,

Lacasta A, Accensi F, Díaz I, Nofrarías M, Pujols J, Blanco E, Pérez- Filgueira

M, Escribano JM, Rodríguez F. Enhancing DNA immunization by targeting

ASFV antigens to SLA-II bearing cells. Vaccine. Jul 26;29(33):5379-85.

2007 P200701023-CReSA Empleo de la hemaglutinina del virus de la peste porcina africana como adyuvante. EU-PCT/ES2008000264 (2009)

2013 Anna Lacasta Marín. Avances en el desarrollo de vacunas

frente a la Peste Porcina Africana. Universitat de Barcelona (UB).

Beca FPU. Nos salvó el proyecto AGL2007-66441-C03-01/GAN.

INVESTIGADORA del ILRI Nairobi (Kenia)

2014 Lacasta A, Ballester M, Monteagudo PL, Rodríguez JM, Salas

ML, Accensi F, Pina-Pedreroa S, Bensaid A, Argilaguet J,

López-Soria S, Huteth E, Le Potier MF, Rodríguez F.

Expression library immunization can confer protection against

African swine fever virus lethal challenge. J Virology. Accepted

2011 P26002ES00-CReSA. Utilización de una construcción génica y/o

peptídica para la fabricación de una vacuna para la prevención y/o tratamiento de la

infección causada por el Virus de la Peste Porcina Africana (VPPA). Spain

www.cresa.cat

2013 Peste porcina africana y vacuna: dos conceptos que no tienen por qué ser

antagónicos. Suis 101; 25-29.

2013 Vaccines against African Swine Fever: Yes, we can! Pig Progress29; 8-10.

2013 Cellular immunity in ASFV responses. Virus Res. Nov 29. pii: S0168-

1702(12)00454-6. doi: 10.1016/j.virusres.2012.11.009.

2012 Standardization of pathological investigations in the framework of

experimental ASFV infections. Virus Res. Jan 11. doi:pii: S0168-

1702(12)00499-6

Representing CReSA in GARA: Global African Swine Fever Research Alliance; a coordinated global research alliance enabling the progressive control and eradication of ASF (http://www.ars.usda.gov/GARA/partners.htm)o Launch Meeting at the Plum Island Animal Disease Center (PIADC) in New

York, April 3-4, 2013. Invited by the USDA

Representing CReSA in EPIZONE; an European Research Group (ERG) that plays a central role in prevention and control of animal diseases and will contribute to limiting both the risks and damage caused by those diseases in the EU and beyond (http://www.epizone-eu.net/)o The 7th EPIZONE Annual Meeting "Nothing permanent, except change"

was held in Brussels, Belgium, 1-4 October 2013 and hosted by CODA-CERVA ; 4 October EFSA Satellite Meeting.

CReSA-Qingdao (bilateral agreement)o Invitation by Dr. Zhilian Wang; Director of the China National Diagnostic

Center for Exotic Animal diseases; Qingdao, China; 12-14 mayo Invitation by Dr. Shan Hu; Dean of the College of Animal Science, Qingdao

Agricultural University; 11 al 15 de Agosto de 2013. o Visit to CReSA: Dr. Shan Hu and Dr. Moreno (SLU); September 23-2013

Visibilidad

Maria Ballester 2007-2010 Programa Consolider Ingenio 2010

(CSD2006-7. 2320)

2011 Ballester M, Rodríguez-Cariño C, Pérez M, Gallardo C, Rodríguez JM,

Salas ML, Rodriguez F. Disruption of Nuclear Organization during the

Initial Phase of African Swine Fever Virus Infection. J Virol. 85:8263-9.

2010 M. Ballester, I. Galindo, C. Gallardo, J.M. Argilaguet, J. Segalés, J.M.

Rodríguez and F. Rodríguez. Intranuclear detection of African swine fever

virus (ASFV) DNA in several cell-types from formalin-fixed and paraffin-

embedded tissues using a new in situ hybridisation (ISH) protocol. Journal

of Virological Methods; 168:38-43.

2014 Sherry Kurtz, Llorenç Grau-Roma, Martí Cortey, Maria Fort, Fernando Rodríguez,

Marina Sibila, Joaquim Segalés Pigs naturally exposed to porcine circovirus type 2

(PCV2) generate antibody responses capable to neutralise PCV2 isolates of different

genotypes and geographic origins. Veterinary Research 45:29.

2014 E Pileri, M Cortey, F Rodríguez, M Sibila, L Fraile, J Segalés Comparison of the

immunoperoxidase monolayer assay and three commercial ELISAs for detection of

antibodies against porcine circovirus type 2. Veterinary journal; May 22

2009 Eva Pérez Martín. Desarrollo de nuevas metodologís para el studio de la patogénesis y elcontrol del circovirus porcino de tipo 2 (PCV2). Calificación Máxima de Sobresaliente CumLaude. niversitat Autónoma de Barcelona (UAB). Spain; Postdoctoral Researcher at ThePirbright Institute (UK)

2009 Pérez-Martín E, Gómez-Sebastián S, Argilaguet JM, Sibila M, Fort M, Nofrarías M,

Kurtz S, Escribano JM, Segalés J, Rodriguez F. Immunity conferred by an

experimental vaccine based on the recombinant PCV2 Cap protein expressed in

Trichoplusia ni-larvae. Vaccine; 154:167-74

2008 Pérez-Martín E, Grau-Roma L, Argilaguet JM, Nofrarías M, Escribano JM, Gómez-

Sebastián S, Segalés J, Rodríguez F. Development of two Trichoplusia ni larvae-

derived ELISAs for the detection of antibodies against replicase and capsid proteins

of porcine circovirus type 2 in domestic pigs. J Virol Methods. 154:167-74.

2007 Pérez-Martín E, Rovira A, Calsamiglia M, Mankertz A, Rodriguez F and Segalés J. A new

method to identify cell types that support porcine circovirus type 2 replication in formalin-

fixed, paraffin-embedded swine tissues. J. Virol. Methods. 146:86-95.

PCV2 (Colaboración con J Segalés-CReSA)

2014 Júlia Vergara-Alert, Núria Busquets, Maria Ballester, Aida J Chaves, Raquel Rivas,

Roser Dolz, Zhongfang Wang, Stephan Pleschka, Natàlia Majó, Fernando Rodríguez,

Ayub Darji The NS segment of H5N1 avian influenza viruses (AIV) enhances the

virulence of an H7N1 AIV in chickens. Veterinary Research 45:7.

2013 Vergara-Allert J, Moreno A, Zabala JG, Bertran K, Costa TP, Cordón I, Rivas, Majó N,

Busquets N, Cordioli P, Rodriguez F, Darji A. Exposure to a Low Pathogenic A/H7N2

Virus in Chickens Protects against Highly Pathogenic A/H7N1 Virus but Not against

Subsequent Infection with A/H5N1. PloS one 8 (3), e58692

2012 Vergara-Alert J, Argilaguet JM, Busquets N, Ballester M, Martín-Valls GE, Rivas R,

López-Soria S, Solanes D, Majó N, Segalés J, Veljkovic V, Rodríguez F, Darji

A. Conserved synthetic peptides from the hemagglutinin of Influenza viruses

induce broad humoral and T-cell responses in a pig model. PLoS

One.7(7):e40524. doi: 10.1371/journal.pone.0040524.

2013 Gómez-Lucia E, Rodriguez F. Research paths to successful prevention and treatment of

swine-derived H1N1 influenza virus infection. Drug News Perspect. ;23:65-70.

Influenza (Colaboración con A Darji-CReSA)

2011 Ganges L, Borrego B, Fernández-Pacheco P, Revilla C, Fernández-Borges N,

Domínguez J, Sobrino F, Rodriguez F. DNA immunization of pigs with foot-and-mouth

disease virus minigenes: From partial protection to disease exacerbation. Virus Res.

157:121-5.

2011 Borrego B, Argilaguet JM, Pérez-Martín E, Dominguez Jr, Pérez-Filgueira, M,

Escribano JM, Sobrino F, Rodriguez F. A DNA vaccine encoding foot-and-mouth disease

virus B and T-cell epitopes targeted to class II swine leukocyte antigens protects pigs

against viral challenge. Antiviral Research 92 (2); 359-363.

2007 Gravier R, Dory D, Rodriguez F, Bouheard S., Beven S, Cariolet R and Jestin A.

Influence of ubiquitin fusion to Pseudorabies virus (Herpes Suid 1) glycoproteins in a

DNA vaccine on swine immunization and protection. Acta Virologica 51(1):35-45.

2006 Borrego B, Fernandez-Pacheco P, Ganges L, Domenech N, Fernandez-Borges N,

Sobrino F and Rodriguez F. DNA vaccines expressing B and T cell epitopes can protect

mice from FMDV infection in the absence of specific humoral responses. Vaccine 24:

3889-3899

2006 Barfoed AM, Rodriguez F, Therrien D, Borrego B, Sobrino F and Kamstrup S. DNA

immunization with 2C FMDV non-structural protein reveals the presence of an

immunodominant CD8+, CTL epitope for Balb/c mice. Antiviral Research 72: 178-189

Aftosa(CReSA; CISA-INIA & CBMSO)

2014 Núria Busquets, Gema Lorenzo, Elena López-Gil, Raquel Rivas, David Solanes,

Iván Galindo-Cardiel, F Xavier Abad, Fernando Rodríguez, Albert Bensaid, George

Warimwe, Sarah C Gilbert, Mariano Domingo, Alejandro Brun. Efficacy assessment

of an MVA vectored Rift Valley Fever vaccine in lambs. Antiviral Research.

2011 Boshra H, Lorenzo G, Rodriguez F, Brun A. A DNA vaccine encoding

ubiquitinated Rift Valley fever virus nucleoprotein provides consistent

immunity and protects IFNAR(-/-) mice upon lethal virus challenge. Vaccine.

15;29(27):4469-75.

2008 Lorenzo G, Martín-Folgar R, Rodríguez F, Brun A. Priming with DNA plasmids

encoding the nucleocapsid protein and glycoprotein precursors from Rift Valley

fever virus accelerates the immune responses induced by an attenuated vaccine in

sheep. Vaccine. 26:5255-62.

2010 Nonito Pagès Martínez. Mosquits del gènere Culicoides: caracteritzaciógenotípica de potencials vectors de la Llengua Blava a Catalunya idesenvolupament de noves eines diagnostiques. Universitat Autónoma deBarcelona (UAB). Investigador Postdoctoral en Guadalupe

Virus del Valle del Rift (CReSA-CISA/INIA)

Priones (CReSA-CISA/INIA)2007 Natalia Fernández Borges. Estimulación específica del sistema inmunológico frente a

enfermedades producidas por priones. Calificación Máxima de Sobresaliente CumLaude. Universidad Complutense de Madrid.. Investigador postdoctoralIKERBASQUE (PAIS VASCO)

2007 Fernandez-Borges N; Brun A, Whitton J L, Parra B, Diaz-SanSegundo F, Salguero F J,

Torres J M and Rodriguez F. DNA vaccination can break tolerance to PrP in normal

mice, and attenuates prion disease after intracerebral challenge J Virology 80: 9970-

9979.

2005 P200500448- CReSA. Procedimiento y estrategias vacunales para potenciar la respuestainmune contra priones tras la vacunaciön, kits de diagnóstico y composicionesfarmacéuticas para la profilaxis y tratamiento de encefalopatías espongiformes. Spain

Peste Porcina Clásica (CReSA &CISA-INIA)2010 Tarradas J, Argilaguet JM, Rosell R, Nofrarías M, Crisci E, Córdoba L, Pérez-Martín E,

Díaz I, Rodríguez F, Domingo M, Montoya M, Ganges L. Interferon-gamma induction

correlates with protection by DNA vaccine expressing E2 glycoprotein against classical

swine fever virus infection in domestic pigs. (2009). Vet Microbiol;142:51-8.

2008 Ganges L, Nunez JI, Sobrino F, Borrego B, Fernandez-Borges N, Frias MTand

Rodriguez F. Recent advances in the development of recombinant vaccines against

classical swine fever virus: Cellular responses also play a role in protection. Vet J.

177:169-77.

2005 Ganges L, Barrera M, Núñez JI, Blanco I, Frias MT, Rodríguez F and Sobrino F. A DNA

Vaccine expressing the E2 protein of classical swine fever virus elicits T cell responses

that prime for rapid antibody production and total protection upon viral challenge.

Vaccine 23:3741-3752

2013 Sultan Gülçe İz, Mert Döşkaya, Ayşe Caner, Aysu Değirmenci Döşkaya, Fernando

Rodriguez, Yüksel Gürüz, S. İsmet Deliloğlu Gürhan A novel dual promoter DNA

vaccine induces CD8+ response against Toxoplasma gondii sporozoite specific surface

protein “SporoSAG” through non-apoptotic cells Trials in Vaccinology. 01/2014; 3:81–

88.

2012 Todolí F, Rodríguez-Cortés A, Núñez Mdel C, Laurenti MD, Gómez-Sebastián S,

Rodríguez F, Pérez-Martín E, Escribano JM, Alberola J. Head-to-head comparison of

three vaccination strategies based on DNA and raw insect-derived recombinant proteins

against Leishmania. PLoS One. 7(12):e51181. doi: 10.1371/journal.pone.0051181

2011 Rivas EI, Driver JP, Garabatos N, Presa M, Mora C, Rodriguez F, Serreze DV,

Stratmann T. Targeting of a T cell agonist Peptide to lysosomes by DNA vaccination

induces tolerance in the nonobese diabetic mouse. J Immunol. 186:4078-87.

2008 Garaude J, Kaminski S, Charni S, Aguilò JI, Jacquet C, Plays M, Hernandez J,

Rodriguez F, Hipskind RA, Anel A, Villalba M. Impaired anti-leukemic immune

response in PKCtheta-deficient mice. Mol Immunol. 45:3463-9.

2008 Theil DJ, Libbey JE, Rodriguez F, Whitton JL, Tsunoda I, Derfuss TJ, Fujinami RS

Targeting myelin proteolipid protein to the MHC class I pathway by ubiquitination

modulates the course of experimental autoimmune encephalomyelitis.. J

Neuroimmunol. 15;204:92-100.

Miscelánea (Nacional e internacional))