8/10/2019 porcentajes de celulas inmunes c57bl6.pdf

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1. Cell type frequencies and percentages are for 6 to 12 weeks old C57BL/6 or C57BL/6J mice. The values will vary depending on mouse strain, age, gender, and environment. 2. Data sourced from:A. The Jackson Laboratory. Flow-cytometric analysis of 11 inbred strains of mice. MPD:Jaxpheno6.

Mouse Phenome Database website, The Jackson Laboratory, Bar Harbor, Maine, USA. http://phenome.jax.org, Aug, 2013; B.The Jackson Laboratory. Hematological survey of 11 inbred strains of mice. MPD:Jaxpheno4. Mouse Phenome Database website, The Jackson Laboratory, Bar Harbor, Maine, USA.

http://phenome.jax.org, Aug, 2013; C.STEMCELL Technologies RND Scientists. 3. Peripheral blood values expressed as cells/L of blood. Spleen and bone marrow values expressed as % of total splenocytes or bone marrow cells. 4. EasySep/RoboSep Immunomagnetic Isolation. 5. Negative selectionlabels unwanted cells to isolate desired untouched cells. 6. Positive selection labels and isolates desired cell types. 7. Without CD43 depletion; for pan-B cells.

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Frequencies & Percentagesof Mouse Immune Cell Types1,2

CELL TYPE TISSUE SOURCE FREQUENCIES OR PERCENTAGES(cells/L of blood or % of total cells)3

STEMCELL TECHNOLOGIESCELL ISOLATION KITS4(CATALOG#)

SELECTION METHOD

NEGATIVE5 POSITIVE6

Erythrocytes Peripheral Blood 10.6 0.4 x 103

Platelets Peripheral Blood 1157 123 x 103

Leukocytes Peripheral Blood 2.6 0.9 x 103

Lymphocytes Peripheral Blood 2.0 0.8x 103

T Cells Spleen 21.3 5.3% 19851 18951 (CD90.2+)

Nave T Cells Spleen 21.3 4.9% 19848

CD4+T Cells Spleen 13.6 1.5% 19852 18952 (CD4+)

Nave CD4+T Cells Spleen 12.6 1.9% 19765

Memory CD4+T Cells Spleen 6.2 1.7% 19767

Regulatory T Cells (CD4+CD25+) Spleen 2.0 0.3% 19782 (CD4+CD25+)

CD8+T Cells Spleen 8.6 0.7% 19853 18953 (CD8a+)

Nave CD8+T Cells Spleen 12.0 2.4% 19858

Memory CD8+T Cells Spleen 3.4 1.0%

NK T Cells Spleen 1.9 0.6%

B Cells Spleen 57.8 7.2%19854 18954 (CD19+)

198447

NK Cells Spleen 1.1 0.2% 19755 18755 (CD49b+)

Monocytes

Spleen 4.2 0.7% 18770 (CD11b+)

Bone Marrow 13.2 2.8%19761

Peripheral Blood 0.04 0.03x 103

Pan-Dendritic Cells Spleen 4.7 2.0% 19763 18780 (CD11c+)

Conventional Dendritic Cells Spleen 4.1 1.7%

Plasmacytoid Dendritic Cells Spleen 0.4 0.2% 19764

NeutrophilsBone Marrow 34.0 9.0%

19762

Peripheral Blood 0.5 0.5x 103

Granulocytes Spleen 1.3 1.4%

Eosinophils Peripheral Blood 0.06 0.05x 103

Basophils Peripheral Blood 0.005 0.006 x 103

Hematopoietic Progenitor Cells(Lin-c-Kit+)

Bone Marrow 0.17 0.03%19856

18757 (CD117+)

18762 (CD93+)

18756 (Sca1+)

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