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SYNTHESIS OF NOVEL ALGINATE-SILVER BASED
NANOCOMPOSITES HYDROGEL BEADS AS AN ANTIFUNGAL AGENT AGAINST
Candida albicans
By:Nasreen Ahmad
Adv. PG Diploma in NanotechnologyCentre of Excellence in Materials
Science (Nanomaterials)Dept. of Applied Physics, AMU,
Aligarh.05/03/2023
Presented at:- NCBL-2013,Lucknow
05/03/2023
PLAN OF TALK
Introduction to Nanotechnology and a brief overview of
the experiment.
Experimental procedures employed.
The characterization techniques used to study the
nanomaterial developed.
The antifungal studies.
The results obtained and their interpretations.
The future prospects of the study.
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INTRODUCTION
Nanotechnology is an emerging trade of science which deals with the materials ranging from sizes 1-100nm.
The properties of particles vary significantly in nanoscale sizes making it interesting for various uses.
Metal nanoparticles (NPs), specially Ag NPs have found its applications in variety of fields including medicine.
Alginate is a well known biopolymer composed of homopolymeric blocks of 1−4-linked β-d-mannuronic acid and α-l-guluronic acid.
The present prepared NPs are the Ag NPs embedded in to the biopolymer hence environment friendly.
The prepared particles show excellent antifungal activities-studied on a pathogen MDR C. albicans.
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10mM AgNO3
(500ml)
0.2% sodium alginate solution
Clear solution
100mM NaOH (dropwise)
Magnetic stirrer50 ͦC, 1 hour
PREPARATION OF Ag NPs
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20mM Cacl20.005%
ammonium persulphate
Pale yellow solution obtained.
Centrifuge
Dried and grinded
Ready for Characterization
PREPARATION OF AgNPs contd.
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CHARACTERISATION STUDIES
• X-ray diffraction• UV- Visible Spectroscopy• Atomic Force Microscopy• Fourier Transmission Infra red Microscopy
Characterization of prepared materials:
Anti-fungal studies:
The Prepared Plate For WELL DIFFUTION ASSAY
The Prepared Plate For DISC DIFFUSION ASSAY
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X-RAY DIFFRACTION
XRD patterns of AgNPs embedded in alginate hydrogel beads (Ag/Ab).
20 30 40 50 60 70 80
200
400
600
800
1000
1200
Inte
nsity
(a.u
.)
2 Theta (Deg.)
• Peaks corresponding to 2θ = 38.2, 44.3, 64.5, and 77.4 show the formation of single crystal Ag NPs
• Average Crystal size = 30.21 nm
{111}
{200}
{220} {311}
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UV-VISIBLE SPECTROSCOPY
0.0
0.5
1.0
1.5
250 350 450 550
30 min15 min10 min5 min
Wavelength (nm)
Abs
orba
nce
UV-visible absorbance spectra of AgNPs and alginate colloidal aqueous solution, range from 250 to 600 nm.
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ATOMIC FORCE MICROSCOPY
A B
AFM images of Ag NPs embedded in alginate hydrogel beads (Ag/Ab). (A) 2D image and (B) 3D image.
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FOURIER TRANSMISSION INFRA RED MICROSCOPY
4000 3500 3000 2500 2000 1500 1000 5000.988
0.992
0.996
1.000
1.004
1.008
Tran
smitt
ance
(%)
Wavenumber (cm-1)
3,428
2, 929
16081415
1315
1090
946
900815
(a)
(b)
FTIR patterns of (a) sodium alginate and (b) Ag NPs embedded in alginate hydrogel beads (Ag/Ab).
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THE ANTI-FUNGAL ACTIVITY ASSESSMENT
(b) (c) (d)
2 µg 4 µg 6µg 8 µg
2 µg 4 µg
(A)
(B)(a)
Anti-candidal activity of different concentrations (µg/mL) of Ag NPs embedded in alginate hydrogel beads (Ag/Ab) against the Candida albicans; (A) Agar well diffusion assay (B) Agar paper disc diffusion assay.
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FUTURE POSSIBILITIES.!
i. Can be used in antifungal or even anti-microbial ointments
ii. Has a future in cleaning of drainages etc.
iii. Is bio-degradable hence good for the environment.iv. An in depth in-vitro and in-vivo studies that will help to identify
Ag NPs’s antifungal potential that can be utilized in the
management of diseases caused by MDR C. albicans isolates.
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REFERENCES
i. saha et al; Photochemical green synthesis of calcium-alginate-
stabilized Ag and Au nanoparticles and their catalytic application to 4-
nitrophenol reduction; Langmuir The Acs Journal Of Surfaces And
Colloids; Volume: 26, Issue: 4; 2010; Pages: 2885-2893
ii. Alt, V.; Bechert, T.; Steinrücke, P.; Wagener, M.; Seidel, P.;
Dingeldein, E.; Domann, E. & Schnettler, R. (2004). An in vitro
assessment of the antibacterial properties and cytotoxicity of
nanoparticulate silver bone cement. Biomaterials, 25, 18, 4383-4391,
ISSN 0142-9612)
iii. A. Nasrollahi, Kh. Pourshamsian, P. Mansourkiaee; Antifungal
activity of silver nanoparticles on some of fungi;international jornal
of nano dimension ; 2011;1
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ACKNOWLEDGEMENTS
We are grateful to the Council of Science & Technology (CST),
Govt. of UP, India for financial support in the form of Centre of
Excellence in Materials Science (Nanomaterials). We are also
thankful to JNMC, AMU, Aligarh for the provision of resources.
We will be failing in our duties if we do not thank the staff of
the Department of Applied Physics, AMU, Aligarh.
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Thank you for your kind attention