laboratorios_bioquímica_201520.doc

7/18/2019 laboratorios_bioquímica_201520.doc

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PRÁCTICAS DE LABORATORIO DE BIOQUIMICAINGENIERÍA AGROINDUSTRIAL

UNIVERSIDAD PONTIFICIA BOLIVARIANA

GUSTAVO ADOLFO HINCAPIE LLANOS

201520

Nº FECHA PRÁCTICAS1 Actii!"! Ac#$%"&2 E't("cci)* !+ "*t$ci"*i*"% ,

!+-$%t("ci)* !+ %# .$!+( i*!ic"!$( / E't("cci)* !+ c"($i!("t$% C"("ct+(i3"ci)* 4#-ic" !+ c"($i!("t$%5 R+"cci$*+% !+ c"("ct+(i3"ci)* !+

.($t+*"%6 D+%*"t#("7i3"ci)* .($t+ic"8 Q#-ic" !+ 7.i!$%9 E-#7%i$*+% , +-#7:+*t+%; .(+."("ci)* !+

-",$*+%"< E't("cci)* !+ ($-+7i*" , ."."*"; +

i!+*ti=ic"ci)* !+ %# "cci)* +*3i->tic"10 P"(!+"-i+*t$ +*3i->tic$




PROGRAMA DE INGENIERIA AGROINDUSTRIAL

MATERIA: LABORATORIO DE BIOQUÍMICA

PRACTICA NUMERO: 1

NOMBRE DE LA PRÁCTICA:

ACTIVIDAD ACUOSAOBJETIVO GENERAL.D+t+(-i*"( 7" "ctii!"! "c#$%" !+ !i=+(+*t+% .($!#ct$% ":($i*!#%t(i"7+%

MARCO TEÓRICO

D+7 ":#" c$*t+*i!" +* #* "7 i-+*t$ !+.+*!+* 7"% .($.i+!"!+% (+$7):ic"% ,!+ t+'t#(" !+ ?%t+; .+($ t"-i?* +% (+%.$*%"7+ +* :("* -+!i!" !+ 7"%(+"cc i$*+% 4#-ic"% ; +*3 i->t i c"% , -ic ($ i$ 7): ic"% ; 4#+ %$* 7"% t (+%.(i*ci."7+% c"#%"% !+7 !+t+( i$($ !+ #* .($!#ct$& E7 ":#" %+ !ii!+ +* 7 i(+, +* 7 i:"!"@ 7" .( i-+(" %+(" 7" *ic" !i%.$*i7+ ."(" +7 c(+ci-i+*t$ !+ 7$%-ic($$(:"*i%-$% $ ."(" i* t+(+*i ( +* 7"% t ("*%=$(-"ci$*+% i!($ 7 t ic"%;4#-ic"%; +*3 i->t ic"% ; + tc &; .#+%t$ 4#+ 7 " %+:#*!" +%t" #*i!" " 7 "%#.+(= ic i+ %)7 i!" , *$ .#+!+ i*t+(+*i( +* +%t$% .($c+%$%& E% !+ci( ; "$+%t+ +%4#+-" %)7$ #*" ."(t+ !+7 ":#" +% c"."3 !+ .($.ici"( +%t$% c"-i$%&P"(" -+!i( ! ic" =("cci)* 7" .( i-+("; 7" !i%.$*i7+ %+ "c#) +7 t?(-i*$"cti i!"! "c#$%"; 4#+ %+ i+*+ +-.7+"*!$ !+%!+ 1<5/; , 4#+ (+.(+%+*t"+7 :("!$ !+ i*t+("cci)* !+7 ":#" c$* 7$% !+->% c$*%tit#,+*t+%; $ 7" .$(ci)*

4#+ +%t> ! i%.$*i7+ +* #* . ($!#ct$ ."(" %#% t+* t"( 7"% (+"cc i$*+% ,"-+*ci$*"!"%& "% c$* "%+ +* +%t+ "7$( %+ .#+!+ .(+!+ci( 7" +%t"i7 i!"!!+ #* "7i-+*t$& E%t+ t?(-i*$ .#+!+ +'.(+%"(%+ !+ 7" %i:#i+*t+ -"*+("

AJ K P L P$ K H&R& L100

L" "ct i i!"! "c#$%" +% #*" . ($. i+!"! i*t ( *%+c" !+ c"!" " 7i-+*t$ ,*ic"-+*t+ %+ (+ 7"c i$*" c$* + 7 c$*t+* i!$ !+ #-+!"! : 7$"7 !+ ! ic$"7 i-+*t$ .$( -+!i$ !+ 7"% 7 7"-"!"% c#("% $ i%$t+(-"% !+ "!%$(c i)* ,!+%$(c i)*@ .$( +%t" ("3)*; +% -#, i-.$(t"*t+ *$ c$*=#*!i ( 7" "ct i i!"!"c#$%" c$* +7 c$*t+*i!$ !+ ":#" ," 4#+ 7" (+7"ci)* *$ +% 7 i*+"7 , .$( +*!+*$ %i:*i=ic"* 7$ -i%-$&

PROCEDIMIENTO:

A. D+t+(-i*"ci)* !+7 C$*t+*i!$ !+ #-+!"! !+ 7" M#+%t(" (Realizaru! ! "!# "$a# a%e# "el la&!ra%!ri!'

1& S#!ii!i ( = i *"-+*t+ 7" -#+%t (" !+7 " 7i-+*t$ .$( %+( "*" 7i 3"!$ &P"(" t" 7 + =+ct$; # t i 7 i 3"( #* c#ci 7 7$ ; #* -$(t+($ c$* . i% t i 7$ $ #*$-$:+*i3"!$( !+ "7t" +7$ci!"!

2& C$7$c"( +* 7" "7"*3" !+ #-+!"! +7 . 7"t i 7 7$ .$(t"-#+%t(" , "#%t"( +7 c+($ !+ 7" "7"*3" c$* 7" .+(i 7 7" !+ c"7 i("ci)*

/& P+%"( 10 :("-$% !+7 "7 i-+*t$; .(+i"-+*t+ $-$:+*i3"!$& L 7+"( 7" -#+%t(" " 7 %+c"!$( $ !$*!+ !+%i!("t+& E7 " 7i-+*t$ *$

!++ * i c"("-+7 i3"(%+ * i c" 7c i*"(%+& P"(" + 7 %+c"!$; %+ .#+!+



+-.7+"( #*" t+-.+("t#(" +*t(+ 60 , 85 C

B & D+ t+( -i *"c i) * ! + 7 " Ac ti i! "! !+ 7 A:# " !+ #* A7 i- +* t$

1& C$7$c"( %#= ic i+*t+ c"*t i!"! !+ !$% %" 7+% !+ (+ =+(+*ci" ; #*" c$*"7t" , $t(" c$* "" "ctii!"! !+ ":#" C#"!($ 1; +* (+ci.i+*t+%!+ 250 -L; c$* t"." +(-?t ic" !+i!"-+*t+ ($ t# 7"!$% c$* + 7*$-(+ !+ 7" %" 7 !+ (+ =+(+*ci" #t i 7 i 3"!" , %# c$((+%.$*!i+*t+"cti i!"! !+ ":#"& A:(+:"( %#= ic i+*t+ ":#" +* c"!" (+ci.i+*t+"%t" $t+*+(; c$*#*t"-+*t+ c$* 7" %"7 #*" -+3c7"%$(+%"t#("!"& C$7$c"( +* .7"t$ .($i%t$ !+ .+(=$("ci$*+% %$(+7" -+3c7" !+ %"7 -"% ":#"; ."(" +it"( +7 c$*t"ct$ !+ 7" -#+%t("c$* 7" %"7 !+ (+=+(+*ci"&

2& C$*%t(#i( %+i% .$(t"-#+%t("% c$* .".+7 !+ "7#-i*i$ , ($t#7"( 7$!+ =$(-" "!+c#"!"& P+%"( ".($' i-"!"-+*t+ 0&1 0 &2 : !+ 7

"7 i-+*t$ !+ c"!" .$(t"-#+%t("& I*t($!#ci( t(+% !+ 7"% -#+%t("%!+ *t ($ ! + c "! " #* $ !+ 7 $% ( +c i. i+ *t+% c $* 7 "% % "7 +% ! +(+=+(+*ci" +%c$:i!"%& T"."(7$% , -"*t+*+( 7$% " t+-.+("t#(""-i+*t+ .$( #* .+(i$!$ *$ -+*$( " 2 $("%& D+%.#?% !+ +%t+t i+-.$; 7" #-+!"! (+7"t i" !+7 "7 i-+*t$ %+ " +4#i 7 i("!$ c$*7" #-+!"! (+7"ti" !+ 7"% %"7+% !+ (+=+(+*ci"&

/& P+%"( !+ *#+$ 7"% -#+%t("% , c"7c#7"( 7" -"%" !+ ":#" .+(!i!"$ :"*"!" .$( c"!" #*" !+ +7 7"%& C"7c#7"( +7 -- !+ ":#":"*"!$ $ .+(!i!$ .$( +7 "7 i-+*t$

& G("=ic"( +7 AJ !+ 7"% %"7+% !+ (+=+(+*ci" c$*t(" +7 .$(c+*t"+ !+

":#" :"*"!$ $ .+(!i!$ c$* 7" -#+%t("& T("3"( #*" 7 *+" (+ct"+* t (+ "-$% .#* t$% @ + 7 i * t+ (c+. t$ !+ 7 + + c$* + 7 AJ !+ 7"-#+%t(" "*"7i3"!" Fi:#(" 1

5& R+c$.i 7"( 7$% (+%#7 t"!$% $t+*i!$% .$( %#% c$-."+($% ."(" 7$%! i=+(+* t+% " 7i-+*t$% "*" 7i 3"!$% , c$*%t (# i( + 7 : (> =i c$ 4#+-#+%t(+ 7" "(i"ci)* !+ 7" "ct ii!"! !+7 ":#" c$* +7 c$*t+*i!$ !+#-+!"! !+ c"!" "7 i-+*t$&

6 & L" "ct i i!"! !+7 ":#" AJ .#+!+ %+( -+! i!" # ti 7i 3"*!$ #*:i()-+t($ +* +7 c#"7 %+ !+t+(-i*" +7 H&R &H#-+!"! R+7"t i"!+ 7" -#+%t (" , !+7 ":#" .#(" 100 H& R& " 7" -i%-"

t+-.+("t#("

8& P"(" +"7#"( 7" +%t"i7 i!"! !+7 .($!#ct$ "7 "t"4#+ !+-ic($$(:"*i%-$% ?"%+ +7 c#"!($ 2

CALCULO ) REULTADO:



Cua"r! 1. A* "e al+ua# #ale#al "e re,ere-ia A* (/0C'

LiB( 0&06LiC7 0&11/

CH/CO2 0&225M:C72 0&/292CO/ 0&/2

M:NO /2 0&52<N"B( 0&566

I 0&60N"NO 2 0&65N"NO / 0&8/N"C7 0&85/

NH2SO 0&90/

B( 0&90<C7 0&9/B"C72 0&<0/NO/ 0&</62SO 0&<8/

Mi-r!!r+ai#! A*

B"ct+(i"% 0&<1L+"!#("% 0&99M$$% 0&90B"ct+(i"% H"7)=i7"% 0&85M$$% '+()=i7$% 0&65L+"!#("% O-)=i7"% 0&60

el -re-iie%! "e Mi-r!!r+ai#!#Cua"r! . A* iia re2ueri"a 3ara

4i+ura 1 & Gr5,i-! "e "e%eria- i6 "e A*



INTRUCCIONE PREVIA OBRE EGURIDADC$*$c+( + 7 -"*+$ !+ 7"% " 7"*3"% , !+ 7 $(*$ !+ %+c"!$ "*t+% !+ %#-"*+$CONTENIDO 7UE DEBE TENER EL IN4ORME:

• REALIAR LA CURVA DE DE AGUA PERDIDA O GANADA VS A;DE LAS SALES CON EL ALIMENTO&

• RESPUESTAS A PREGUNTAS O EERCICIOS PLANTEADOS&BIBLIOGRA48 A A CONULTAR O DE APO)O

1& B"!#i; S& Q#-ic" !+ 7$% A7i-+*t$%& E!it$(i"7 A7"-("M+'ic"*"; S& A& M?'ic$; 1<91@ .. 29 //

2& H+((+("; C& M"*#"7 !+ L"$("t$(i$ E"7#"ci)* !+ 7" c"7i!"! !+7.+%c"!$; -"(i%c$% , .($!#ct$% .+%4#+($%& UNA& H+(+!i";19<9@ 668



MATERIA BIOQUÍMICAPRACTICA NUMERO: NOMBRE DE LA PRACTICA E't("cci)* !+ "*t$ci"*i*"% , !+-$%t("ci)* !+%# .$!+( i*!ic"!$( OBJETIVO GENERAL A*"7 i3"( 7$% c$*c+.t$% !+ 7$% i*! ic"!$(+% ."("(+"cci$*+% i$4#-ic"% c$* "%+ +* +7 .$!+( !+ c"-i$ c$7$( i-?t( ic$ !+7"% "*t$ci"*i*"% "$ c$*!ici$*+% !+ .H&PROCEDIMIENTO T(i t#("( , -"c+("( ".($' i-"!"-+*t+ 200 : ("-$% !+ (+.$7 7$ -$("!$"!ici$*"*!$ 20 -L !+ "c+t"t$ !+ .7$-$ 0&1 M; ->% 0&5 -L !+ i!()'i!$ !+

"-$*i$ "7 10 &



Fi7t("( +* t+7" =i 7 t("*t+&

C+*t(i =#:"( " 2500 (.- .$( 5 -i*#t$% , !+%+c"( +7 %$(+*"!"*t+&

L""( +7 .(+ci.it"!$ c$* 25 -L !+ +t"*$7&

D+ c"* t" ( + 7 7 4# i!$ , " "! i( 15 - L ! + .( $. "* $7 - >% 1 - L ! + HC7c$*c+*t("!$&

C+*t(i =#:"( *#+"-+*t+& S+ =$(-" #* .(+ci.it"!$ !+ c7$(#($ !+ .7$-$&

D+c"*t"( +7 7 4#i!$ c$7$(+"!$ +* #* +-#!$ !+ %+."("ci)*&

A"!i( 15 -L !+ cic7$+'"*$ "7 +-#!$&

Si *$ %+ %+."("* 7"% ="%+% ""!i( 0&5 -L !+ ":#"&

R+c$:+( 7" ="%+ "c#$%" i*=+(i$(&Di%.$*+( 7$% t#$% !+ +*%",$ c$* t"-.)* =$%="t$ " !i=+(+*t+% PH 2&11

A"!i( 5 :$t"% !+7 +'t("ct$ " c"!" t#$ !+ +*%",$&

O%+("( c"-i$% !+ c$7$(&

P(#+"% c$* %$7#ci)* t"-.)* =$%="t$ 10 -L t$t"7&

P(#+" HCL 0&1 N POH2

0&15M PON"2H0&15M PO/ $PON"/

0&15M

.H .H (+"7

1 <&5 0&5 2&1

2 0&5 <&5 /&6

/ 10&0 &8

<&5 0&5 5&6

5 <&0 1&0 5&<

6 9&0 2&0 6&2

8 8&0 /&0 6&59 6&0 &0 6&6

< 5&0 5&0 6&9

10 &0 6&0 8&0

11 /&0 8&0 8&2

12 2&0 9&0 8&

1/ 1&0 <&0 8&8

1 &5 5&5 9&0

15 5&0 5&0 <&9

16 /&0 8&0 10&8



18 /&0 8&0 11&2

19 N"OH 1010-7 1&0

MATERIAL A TRAER:

REPOLLO MORADOINTRUCCIONE PREVIA OBRE EGURIDAD:L++( 7" +ti4#+t" !+ c"!" (+"cti$; !+t+(-i*"*!$ 7"% *$(-"% !+ %+:#(i!"!!+ c"!" #*$&CONTENIDO 7UE DEBE TENER EL IN4ORME: ANÁLISIS DE RESULTADOS SOLUCIWN AL CUESTIONARIO

Cue#%i!ari! #!&re a%!-iaia# 9 #u 3!"er i"i-a"!r "e P.

Q#? %i:*i=ic"* C$7$(C$7$("*t+Pi:-+*t$&

D+%c(i" 7"% c"("ct+(%tic"% 4#-ic"% !+ 7$% c"($t+*$% , %# c"."ci!"!c$-$ "*ti$'i!"*t+%&

L$% "*t$ci"*$% "*t$ci"*i*"% %$* #%"!$% +* 7" i*!#%t(i" "7 i-+*t"(i"& D+!$*!+ %+ +'t("+* ."(tic#7"(-+*t+ , c#>7 +% %# #%$X

BIBLIOGRA4IA A CONULTAR O DE APO)O L$(i+*t& Bi$4#-ic" ":($i*!#%t(i"7& ACRIBIA&1<<



MATERIA: LABORATORIO DE BIOQUÍMICA

PRACTICA NUMERO: ;

NOMBRE DE LA PRACTICA:ETRACCIWN DE CARBOHIDRATOS

OBJETIVO GENERAL: A.(+*!+( 7$% -?t$!$% :+*+("7+% ."(" 7" +'t("cci)* !+ 7$% c"( $i!("t$%&

OBJETIVO EPECI4ICO:• E't("+( 7" %"c"($%" ."(ti+*!$ !+ 7" c"" !+ "3c"(&• E't("+( 7" 7"ct$%" ."(ti+*!$ !+7 7"ct$%#+($ !+ 7" 7+c+&• E't("+( +7 :7#c):+*$ ."(ti+*!$ !+7 :"!$ !+ (+%&• E't ("+( 7" = (#c t$%" ."( t i+*!$ !+ 7"% = (#t"% c$-$ #"%; *"("* " ,

"*"*$&ROCEDIMIENTO:

" E't("cci)* !+ 7" 7"ct$%"

A 100 -L !+ 7+c+ "!ici$*"( .$c$ " .$c$ HC7 !i 7#i!$; "%t" 4#+ +7 .H !+ 7"-+3c7" " 7c"*c+ " +%t"( +*t(+ &6 , &9 & D+"( +* (+.$%$ ."(" ="c i 7 i t"( 7"%+."("ci)* !+ 7" c#""!" , +7 7"ct$%#+($&Fi 7 t("( %$(+ t+7" !$7+ $ .".+7 = i 7 t ($ c#"7 i t"t i$ ."(" %+."("( 7" c"%+*" ,



.($c+!"-$% c$* +7 %#+($ =i 7 t("!$& A!ici$*"( C"OH 2 " 7 10 " +% t+ %#+($ c$* t$!$ c# i!"!$ , ": i t"c i)*%#"+; "%t" "7c"*3"( #* .H !+ 6&2&C"7+*t"( 7" -+3c7" !+ %#+($ "%t" +#7 7 ic i)* , !+"( +*=( i"( ."(" 4#+ 7"%.($ t+*"% , : ("%"% (+% i!#" 7+%; 7" -"t+( i" -i*+("7 , $ t("% %#% t"*ci"%=7$c#7+* , =7$t+*& A!ici$*"( "7:$ !+ !i)'i!$ !+ c"($*$ ."(" +7 i-i*"( +7 +'c+%$ !+ i!()'i!$!+ c" 7c i$ ; !+c"*t"( , = i 7 t ("( c$* ". 7 ic"c i)* !+ "c$ % i =#+(+ *+c+%"( i$ &G#"(!"( ."(t+ !+ !ic" -#+%t(" ."(" .()'i-" c"("ct+(i3"ci)*&

E't("cc i)* !+ G7#c):+*$

- C$(t"( +* t($3$% .+4#+$% 10 : !+ :"!$ =(+%c$ !+ (+%&- t( i t#("( +* #* -$(t+($ c$* ",#!" !+ #* .$c$ !+ "(+*" .(+i"-+*t+

7""!"&- P"%"( 7$ t( i t#("!$ " #* +"Y+( !+ 250 -L , ":(+:"( c$* c#i!"!$ OH

"7 /0 +* =$ (-" 4#+ +4# i" 7:" " c#" t ($ +c+% + 7 $ 7#-+* !+ 7"

-#+%t("&- A:it"( c$* #* ":it"!$( !+ i!( i$ 7$ "*t+( i$( , 7 7+"( " c"7+*t"-i+*t$ ,

+# 77 ic i)* %$ (+ #* "$ -"( " .$( 5 - i*#t$% c$* ":i t"ci )*c$*%t"*t+&

- Fi7t("( +* c"7 i+*t+ " t("?% !+ #* 7 i*$ :"%" c$* ",#!" !+ "c$&- L""( +7 (+%i!#$ c$* #* .$c$ !+ OH- $t+ +7 (+%i!#$ 7#+:$ !+7 7""!$- E*=(i"( +7 = i 7 t("!$ %$(+ +7 i+7$- A:(+:"( 1 -L !+ N" 2 SO %"t#("!$ c$-$ c$.(+ci.it"*t+&- M+3c7"( -#, i+*- P(+ci.i t"( +7 : 7#c):+*$ .$( "!ici)* !+ 2 +c+% +7 $7#-+* !+ +t"*$7

!+7 <6

- C$7$c"( 7" -#+%t(" +* t#$% !+ c+*t(=#:"- D+"( 7" -#+%t(" +* "$ !+ i+7$ .$( 5 -i*#t$%- C+*t(i =#:"( " "" +7$ci!"! .$( / -i*#t$%- D+%c"(t"( +7 %$(+*"!"*t+- R+#*i( , !i%$7+( +7 .(+ci.it"!$ !+7 :7#c):+*$ +* 20 -7 !+ ":#" t ii"- G#"(!"(7" ."(" c"("ct+(i3"ci)*&

cE't("cci)* !+ S"c"($%"

- Lic#"( c$* ",#!" !+ #* .$c$ !+ ":#" c" 7 i+*t+ " 7:#*$% t ($3$% !+c""

- R+c$:+( +7 .($!#ct$ !+%i*t+:("!$ +* #* +"Y+( !+ 250 -L&

- A!ici$*"( 50 -L !+ ":#" !+%ti7"!" c"7 i+*t+ "7 t+i!$ !+%i*t+:("!$&- A:it"( .$( #*$% / 5 -i*#t$% " = i* !+ 7""( 7" %"i" $ #:$ "!+(i!$

" 7"% =i("% , ="ci 7 i t"( 7" -+$( +'t("cci)* !+7 "3c"(&- P"%"( +%t" -+3c7" " t("?% !+ t+7" !$7+&- C7"( i = ic"( + 7 = i 7 t ("!$ !+ 7 #:$ -+!i"*t+ "! ic i)* !+ C"OH 2 " 7 10

"%t" $t+*+( .H 9 9&5&- C"7+*t"( "%t" +#77 ici)* c$* ":it"ci)* c$*%t"*t+&- R+.$%"( , +*=(i"( ."(" 7$:("( = 7$c# 7"c i)* !+ ! i+(%"% %#%t"*ci"%

.(+%+*t+% +* + 7 #:$; t" 7+% c$-$ .($t+*"%; :("%"%; :$-"%; c+("%;

.+cti*"%; >ci!$% $(:>*ic$%; -i*+("7+% , $t($% c$-.$*+*t+% !+7 t+ i!$+:+t"7&

-

Fi7t("( c$* ",#!" !+ "c$&- G#"(!"( ."(" c"("ct+(i3"ci)*&



!E't("cci)* !+ 7" =(#ct$%"

- Lic#"( #*" -#+%t(" !+ =(#t$ -"!#($ c$* %#=ic i+*t+ c"*ti!"! !+ ":#"c"7i+*t+&

- C"7+*t"( 7" -+3c7" +* #* +"Y+( !+ 250 -L "%t" c"%i +#77 ici)*&- Fi7t("( +* c"7 i+*t+ c$* ",#!" !+ "ci$&- C7"( i = ic"( + 7 = i 7t ("!$ -+!i"*t+ "! ic i)* !+ C"OH2 " 7 10 "%t"

$t+*+( PH ≅ 9 9&5- R+.$%"( , +*=( i"( ."(" 7$:("( = 7$c#7"ci)* !+ 7"% !i+(%"% %#%t"*ci"%

.(+%+*t+%&- Fi7t("( c$* ",#!" !+ "c$&- G#"(!"( ."(" c"("ct+(i3"ci)*&-

NOTA L"% -#+%t("% !++* %+( ($t#7"!"% , :#"(!"!"% +* 7" *++(" ."(" 7".()'i-" .(>ctic"&

INTRUCCIONE PREVIA OBRE EGURIDAD:

• EN EL MANEO DE LA CENTRÍFUGA; COLOCAR LO S TUBOS DEENSAO EN EL PORTATUBOS DE MANERA QUE ZSTA QUEDECOMPENSADA; SI Q UEDAN DOS ESPACIOS VACIOS COLOCARDOS TUBOS DE ENSAO CON AGUA; CO N EL FIN DE EVITARVIBRACIONES FUERTES E INCOMODAS&

• CADA VE QUE SE UTILICE EL POTENCIOMETRO DEBE LAVARSEEL ELECTRODO ANTES DESPUZS DE CADA LECTURA; CON AGUA DESTILADA; LUEGO DEARLO EN ELLA&

• TENER EN CUENTA LAS NORMAS DE SEGURIDAD EN CUANTO ALA MANIPULACIWN DE LOS REACTIVO S&

CONTENIDO 7UE DEBE TENER EL IN4ORME:

REPUETA A CUETIONARIO

o Q#? %+ +*ti+*!+ .$( !i:+(ii 7 i!"! $ !i:+%tii 7 i!"! !+ 7$%c"($i!("t$%&

o E'.7i4#+ 7"% t(+% =#*ci$*+% -+t")7ic"% !+ 7$% c"($i!("t$%+* 7$% "*i-"7+%&

o Q#? +% 7" =i(" , c#>7 +% % # =#*ci)* !+*t($ !+ #*" !i+t""7"*c+"!"&

o C#>7 +% 7" =#+*t+; c$-.$%ici)* , #%$ !+ 7$% %i:#i+*t+%

c"($i!("t$% A7-i!)*; c+7#7$%"; .+cti*"&o C)-$ %+ .($!#c+ 7" ."*+7"&

BIBLIOGRA48A A CONULTAR O DE APO)O :BRAVERMAN; &B&S& I*t ($!#cc i)* " 7" B i$4# -ic" !+ 7$% A 7i-+*t$%&M+'ic$& 1&<95&VARGAS O; +*c+%7"$& F#*!"-+*t$% !+ Ci+*ci" A7i-+*t"(i"& U*i+(%i!"!N"ci$*"7 !+ C$7$-i"& B$:$t>& 1&<98&

UNIVERSIDAD PONTIFICIA BOLIVARIANAPROGRAMA DE INGENIERIA AGROINDUSTRIAL



MATERIA LABORATORIO DE BIOQUÍMICA

PRACTICA NUMERO: <

NOMBRE DE LA PRACTICA:CARACTERIACIWN QUÍMICA DE CARBOHIDRATOSOBJETIVO GENERAL:C$*$c+( 7$% -?t $!$% :+*+( "7+% ."(" 7" c"("ct +( i3"c i)* !+ 7$%c"($i!("t$%&

OBJETIVO EPECI4ICO:

• C"("ct+( i3"( 4#-ic"-+*t+ 7$% c"($i!("t$% +'t(" !$% !+ =#+*t+%*"t#(" 7+% 7"ct$%"; %"c"($%"; = (#ct$%"; : 7#c):+*$ c$-$ t"-i?*%$7#ci$*+% !+ "3c"(+%; =(+*t+ " -#+%t("% ."t($*+%&

• I!+*ti= ic"( c#"7i t"ti"-+*t+ #* "3c"( (+!#ct$( !+ #*$ *$ (+!#ct$(&• R+" 7i 3" ( . (#+"% 4#+ .+(-i t+* ! i=+(+*ci"( -$*$%"c>( i!$% !+

!i%"c>(i!$%&• Di=+(+*ci"( c#"7 it"t i"-+*t+ +*t(+ #*" "7!$%" , #*" c+t$%" , +*t(+

#*" "7!$+'$%" , #*" "7!$.+*t$%"&PROCEDIMIDIMIENTO:

A& C"("ct+( i3"ci)* !+ 7" 7"ct$%" T$- + 5 -7 !+ #*" %$7#ci)* !+ 7"ct$%", !i%t( i,"7$% +* / t#$% !+ +*%",$ ($t#7>*!$7$% c$* 7$% N$& 1; 2; /(+"7ic+ 7"% %i:#i+*t+% .(#+"%

1[ P(#+" !+ M$7i%

A7 t#$ N$& 1 ""!" 2 :$t"% !+7 (+"cti$ !+ M$7i% A!ici$*+ 1 -L !+ H 2SO c$*c+*t("!$; +%t$ !++ "c+(%+ i*c7 i*"*!$7" . i.+t" , !+ "*!$ !+%7i3"( + 7 >c i!$ .$( 7"% ."(+!+% !+ 7 t#$ !++*%",$&L" =$(-"ci)* !+ #* c$7$( .(.#("i$7+t" +* 7" i*t+(="%+ !+ 7$%74#i!$% +% .(#+" .$%iti" ."(" c"($i!("t$%&

NOTA *$ ":it"( +7 t#$ ."(" 4#+ %+ =$(-+* !$% c"."% 74#i!"%&

2[ .(#+" !+ F+7i*:M+3c7+ 1 -L !+ F+7i*: A , 1 -L !+ F+7i*: B +* #* t#$ !+ +*%",$

, "!ici$*+ "7 T#$ N$& 2C$7$4#+ +7 t#$ +* #* "$ !+ ":#" i(i+*!$ .$( / -i*#t$%O%+(+ 7" c$7$("ci)* ($$ 7"!(i 77 $; c$-$ .(#+" .$% it i " ."(""3c"(+% R+!#ct$(+%&

/[ .(#+" !+ B"(=$+! A7 t#$ N$& / "!ici$*+ 2 -7 !+7 (+"cti$ !+ B"(=$+!C"7i+*t+ +* #* "$ !+ ":#"& Si %+ =$(-" #* .(+c i.i t"!$ " ->% !+!$% -i*#t$% !+ +(i( +% #* !i%"c>(i!$&

B& P(#+" !+ F+7i*: i!+*ti = ic" "3c"(+% (+!#ct$(+%&• E* #* t#$ !+ +*%",$ -+3c7+ 1 -L !+ F+7i*: A c$* 1 -L !+ F+7i*:

B&• A!ici$*+ 1 -7 !+ S$7#ci)* !+ :7#c$%"



• C"7i+*t+ "7 "$ -"(" .$( 5 -i*#t$%• O%+(+ 7" c$7$("ci)*; "*$t+&

C& P(#+" !+ B+*+!ict ."(" "3c"(+% (+!#ct$(+%• C$7$4#+ 1 -L !+ 7 (+"c t i $ !+ B+*+! ic t +* #* t#$ !+ +*%",$ ,

"!ici$*+ 1 -L !+ "3c"( " "*"7i3"( :7#c$%"; =(#ct$%"; 7"ct$%"• C$7$4#+ +* #* "$ !+ ":#" i(i+*!$ ' 5 -i*#t$%• O%+(+ , "*$t+

NOTA %i +%t" .(#+" %+ "*"7i3" c$* %$7#ci$*+% c(+ci+*t+% !+ 7$% "3c"(+%;%+ *$t" 7" %+*%ii 7 i!"! !+ +7 7"&

D& P(#+" !+ M$7 i% ."(" "3c"(+% +* :+*+("7 &• C$7$4#+ 1 -L !+7 (+"cti$ !+ M$7i% +* #* t#$ !+ +*%",$• A!ici$*+ 1 -L !+7 "3c"( " "*"7i3"( :7#c$%"; -"7t$%"&• M+3c7+ , !+ + c"+( 7+*t"-+*t+ , % i* ": i t"( #*"% :$t"% !+ H2SO

c$*c+*t("!$ "%t" 4#+ %+ =$(-+* !$% c"."%&• O%+(+ c#i!"!$%"-+*t+ c#"74#i+( c"-i$ !+ c$7$( +* 7" i*t+(="%+ !+

7$% !$% 7 4#i!$%& A*$t+&• R+.it" +7 .($c+!i-i+*t$ %i 7$ !+%+" #%"*!$ ":#" +* +3 !+ %$7#ci)*

!+ c"($i!("t$& Q#? %"c"% c$-$ c$*c7#%i)*X A*$t"&

E& P(#+" !+ B"(=$+! i!+*ti = ic" -$*$%"c>(i!$%• C$7$4#+ 2 -L !+ 7 (+"ct i$ !+ B"( =$+! +* #* t#$ !+ +*%",$ ,

" !ic i$ *+ 1 - L ! + 7 " % $7 #c i) * ! +7 "3 c "( " " *" 7i3 "( (+ "7i c+%i-#7t>*+"-+*t+ c$* :7#c$%" , 7"ct$%" $ =(#ct$%" , -"7t$%"

• C"7i+*t+ +* #* "$ !+ ":#" i(i+*!$ .$( / -i*#t$%&•

R+t i (+ + 7 t#$ !+ +*%",$ , c$* t (" #* =$*!$ *+:($ $ 7"*c$ i+*i 7#-i*"!$; $%+(+ %i %+ " .($!#ci!$ #* .(+ci.i t"!$ ($$ i*!ic"t i$!+ 7" .(+%+*ci" !+ :7#c$%" , $t($% -$*$%"c>(i!$%&

• Fi 7 t ("( %$(+ .".+7 !+ = i 7 t ($ , *$t+ % i ", .(+c i. i t"!$ ($ $ %$(+ + 7=i 7 t($ , 7"% ."(+!+% !+7 t#$&

• Si 7" -"7 t$%" , 7"c t$%" ! i+ ($* (+%# 7t"!$ .$% it i $ +% c$*+*i+* t+(+.+ti( 7" .(#+" !i 7#,+*!$ ->% %#% %$7#ci$*+% , c"7+*t"*!$ .$( /-i*#t$%&

F& P(#+" !+ S+7 iJ"*$== i!+*ti= ic" c+t$%"% !+ "7!$%"%• C$7$4#+ 1 -L !+7 (+"ct i$ !+ S+7 iJ"*$== +* #* t#$ !+ +*%",$ ,

"!ici$*+ 1 -L !+ 7" %$7#ci)* !+7 "3c"( " "*"7 i3"( (+"7 ic+ 7" .(#+"%i-#7t>*+"-+*t+ c$* =(#ct$%" , :7#c$%"&

• C"7i+*t+ 7" -+3c7" +* #* "$ !+ ":#" i(i+*!$ .$( / -i*#t$%&• O%+(+& A*$t+&

G& P(#+" !+ Bi"7 ! i =+(+*ci" +'$%"% !+ .+*t$%"%• C$7$4#+ 1 -L !+7 (+"cti$ !+ Bi"7 , "!ici$*+ 1 -L !+ 7" %$7#ci)* !+7

"3c"( " "*"7i3"( (+"7 ic+ 7" .(#+" %i-#7t>*+" c$* 'i 7$%" , :7#c$%"• C"7i+*t+ +* #* "$ !+ ":#" i ( i+*!$ .$( -i*#t$% $ "%t" 4#+

+-.i+c+ " +(i(&• L" "."(ici)* !+ #*" c$7$("c i)* "3# 7 +(!$%" i*! ic" (+%#7 t"!$%

.$%iti$%&• E*=(+ +7 t#$ , ":(+:#+ 1 -7 !+ "7c$$7 "-7 ic$&• A:it+; $%+(+ , "*$t+&




• TENER EN CUENTA LAS NORMAS DE SEGURIDAD AL UTILIAR

CADA UNO DE LOS REACTIVOS; PUESTO QUE ESTOS ESTANPREPARADOS CON COMPUESTOS ORGANICOS CADA UNO CONDIFERENTES GRADOS DE TOICIDAD; ACIDE; ETC&

• EN CASO DE ALGUNA QUEMADURA LAVARSE CON SUFICIENT E AGUA&

TABLA DE DATO:C"($i!("t$ M$7i% B"(=$+! F+7 i*: B+*+!ict S+7 iJ"*$= = Bi" 7


• HACER UNA TABLA CON LAS DIFERENTES PRUEBAS ANOTAR SIDIO POSITIVA O NEGATIVA EL COLOR OBSERVADO

• RESPUESTAS A LAS PREGUNTAS O EERCICIO S PLANTEADOS&BIBLIOGRA48 A A CONULTAR O DE APO)O

PLUMMER; D"i!& T& Bi$4#-ic" P(>ctic"& 5[ E!ici)*& E!it$(i"7 McG("J Hi77L"ti*$"-+(ic"*" S&A&& B$:$t> 1<95&

VARGAS; +*c+%7"$& F#*!"-+*t$ !+ Ci+*ci" A7i-+*t"(i"& 2[ +!ici)*& U*i+(%i!"!N"ci$*"7 !+ C$7$-i"& B$:$t> 1<95&

VERA CARDENAS; $%? A(:+-i($& G#" ."(" +7 7"$("t$(i$ !+ Q#-ic" !+ A7i-+*t$%&U*i!"! #*i+(%it"(i" !+7 S#( !+ B$:$t>& B$:$t> 1<<0&




PRACTICA NUMERO: /



NOMBRE DE LA PRACTICA REACCIONES DE CARACTERIACIWN DE PROTEÍNAS

OBJETIVO GENERAL:C$*$c+( 7$% -?t$!$% :+*+("7+% ."(" 7" c"("ct+(i3"ci)* !+ 7"% .($t+*"%&

OBJETIVO EPECI4ICO:

• C"("ct+(i3"( 4#-ic"-+*t+ "7:#*"% %$7#ci$*+% !+ .($t+*"% , "-i*$>ci!$%; "7"c+(7"% (+"cci$*"( c$* (+"cti$% c"("ct+(%tic$% c$* 7$% c#"7+% =$(-"* .($!#ct$%c$7$(+"!$%&

• R+c$*$c+( 7" .(+%+*ci" !+ .($t+*"% , "-i*$>ci!$% +* %$7#ci$*+% !+ "7-i*"!+ #+$; :+7"ti*"; c"%+*" !+ 7" 7+c+&

PROCEDIMIENTO:

A& R+"cci)* !+ 7" *i*i!(i*" ."(" 7$% "-i*$>ci!$%& L" *i*i!(i*"; #* ":+*t+ $'i!"*t+

.$!+($%$; (+"cci$*" c$* t$!$% 7$% "-i*$>ci!$% " #* .H +*t(+ , 9 ."(" !"( #*c$-.#+%t$ !+ c$7$( .(.#("& E%t" (+"cci)* %+ +=+ct" t"-i?* c$* "-i*"% .(i-"(i"% ,"-$*i"c$ .+($ %i* !+%.(+*!i-i+*t$ !+ CO2& L$% "-i*$>ci!$% .($7i*" + i!($'i.($7i*"t"-i?* (+"cci$*"* c$* 7" *i*i!(i*"; .+($ +* +%t+ c"%$ %+ $ti+*+ #* c$7$( "-"(i77$ +*+3 !+7 .(.#("&

L" (+"cci)* +% -#, %+*%i7+ , +% 7" i!+"7 ."(" 7" !+t+cci)* !+ "-i*$>ci!$% +*c($-"t$:("-"% , %# !+t+(-i*"ci)* c#"*tit"ti" +* =("cci$*+% !+ c$7#-*"%&

P($c+!i-i+*t$ c$7$4#+ 1 -L !+ 7" %$7#ci)* !+ "-i*$>ci!$ c"%+*" !+ 7" 7+c+ $"7-i*" $t+*i!" !+ 7" c7"(" !+ #+$ +* #* t#$ !+ +*%",$; ":(+:#+ 1 -L !+ %$7#ci)*!+ *i*i!(i*" , !++ +(i( .$( 2 -i*#t$%& O%+(+ , "*$t+ 7" c$7$("ci)* $t+*i!"&

NOTA (+"7ic+ t"-i?* c$* c#"74#i+(" !+ 7"% %i:#i+*t+% %$7#ci$*+% t(i.t)="*$; =+*i7"7"*i*";ti($%i*"&

B& R+"cci)* '"*t$.($t?ic"& L$% "-i*$>ci!$% 4#+ c$*ti+*+* #* *c7+$ "($->tic$ =$(-"**it($!+(i"!$% !+ c$7$( "-"(i77$ c#"*!$ %+ c"7i+*t"* c$* >ci!$ *t(ic$ c$*c+*t("!$&L"% %"7+% !+ +%t$% !+(i"!$% %$* !+ c$7$( *"("*"; "7 %+( "7c"7i*i3"!$% c$* N"OH&E%t" (+"cci)* i*!ic" 7" .(+%+*ci" !+ 7$% "-i*$>ci!$% "($->tic$% =+*i7"7"*i*"; ti($%i*";t(i.t)="*$&

P($c+!i-i+*t$ " 2 -L !+ %$7#ci)* !+ "-i*$>ci!$%; "!ici$*+ 1 -L !+ HNO/ c$*c+*t("!$&C"7i+*t+ 7+*t"-+*t+ !#("*t+ 1 -i*#t$; +*=(+ "$ +7 c$(($ !+ ":#" =(" , ":(+:#+ :$t" ":$t" N"OH "7 0 "%t" 4#+ 7" %$7#ci)* %+" =#+(t+-+*t+ "7c"7i*"& A*$t+ 7$% c"-i$% !+

c$7$(&

NOTA (+"7ic+ 7" .(#+" c$* c#"74#i+(" !+ 7"% %$7#ci$*+% !+ "-i*$>ci!$% t(i.t)="*$ $="*i7"7"*i*" $ "7"*i*" , c$-."(+ c$* %$7#ci$*+% !+ c"%+*" $ "7-i*"&

C& P(#+" !+ Bi#(+t ."(" +*7"c+% .+.t!ic$%& E7 %#7="t$ "7c"7i*$ !+ c$(+ (+"cci$*" c$*c$-.#+%t$% 4#+ c$*ti+*+* !$% $ ->% +*7"c+% .+.t!ic$% !"*!$ #* c$-.7+$ !+c$7$("ci)* i$7+t"& L" i*t+*%i!"! !+7 c$7$( $t+*i!$ +% .($.$(ci$*"7 "7 *-+($ !++*7"c+% .+.t!ic$% .(+%+*t+% +* 7" .($t+*"& Si* +-"(:$ +%t" (+"cci)* t"-i?* 7".#+!+* .(+%+*t"( c$-.#+%t$% 4#+ t+*:"* !$% :(#.$% c"($*i7$ #*i!$% .$( #* >t$-$!+ *it():+*$ $ !+ c"($*$&

P($c+!i-i+*t$ " 2 -L !+ 7"% -#+%t("% ":(+:#+ 1 -7 !+ Bi#(+t , ":it+ i:$($%"-+*t+ ,$%+(+ 7$% c$7$(+% =$(-"!$%& A*$t+



NOTA (+"7ic+ 7" .(#+" c$* %$7#ci)* !+ "7-i*" $ c"%+*" !i%#+7t" +* #* .$c$ !+ N"OH!i7#i!$; (+.it" 7" .(#+" c$* #* -"c+("!$ !+ -"* $ =($7 !i7#i!$ " 5 -7 !+ ":#" !+%ti7"!"&P"(" .(+."("( %$7#ci)* !+ "7-i*"; %+."(+ 7" c7"(" !+ #* #+$ , !+%.#?% !+ "ti(7"!#("*t+ #*$% -i*#t$% -?3c7+7" c$* ci*c$ +c+% %# $7#-+* !+ ":#"&

D& R+"cci)* !+ H$.Yi*% C$7+& E%t> =$(-"!$ .$( #*" -+3c7" !+ %$7#ci)* c$*c+*t("!"!+ H2SO c$* #* "7!+!$; +7 c#"7 =$(-" #* .($!#ct$ !+ c$7$( *!i:$ "7 (+"cci$*"( c$* +7 t(i.t)="*$&

P($c+!i-i+*t$ " 2-L !+ c7"(" !+ #+$; %i* !i7#i( ":(+:#+ 2 -L !+ >ci!$ "c?tic$ :7"ci"7;7#+:$ !++ c"+( 7+*t"-+*t+ 1 -L !+ H2SO c$*c+*t("!$ .$( 7"% ."(+!+% !+7 t#$; "%t"4#+ %+ =$(-+* !$% c"."%& O%+(+ +7 c"-i$ !+ c$7$( +* 7" i*t+(="%+ %i %+ =$(-" #* "*i77$i$7+t" +* 7" i*t+(="%+ !+ 7$% !$% 74#i!$%; 7" (+"cci)* %+ c$*%i!+(" .$%iti"&

E& R+"cci)* !+ Mi77)*& L$% c$-.#+%t$% 4#+ c$*ti+*+* +7 ("!ic"7 i!()'i+*c+*$(+"cci$*"* c$* +7 (+"cti$; +7 c#"7 +% #*" -+3c7" !+ H: NO/2 +* #*" %$7#ci)* !+

>ci!$ *t(ic$ "7 15 & L$% *ic$% "-i*$>ci!$% =+*)7ic$% !+t+ct"!$% c$* +%t+ (+"cti$%$* 7" ti($%i*" , %#% !+(i"!$% , %$7$ +77$% !"* #* c$-.7+$ !+ c$7$( ($$ c"("ct+(%tic"!+ (+"cci)*&

P($c+!i-i+*t$ " 1 -L !+ 7" %$7#ci)* !+ "-i*$>ci!$% :7ici*"; ti($%i*" $ =+*i7"7"*i*"""!" 5 :$t"% !+7 (+"cti$ !+ Mi77)* , c"7i+*t+ +* #* "$ !+ ":#" c"7i+*t+ .$( 10-i*#t$%& D++ +*=(i"( " t+-.+("t#(" "-i+*t+ , ":(+:#+ 5 :$t"% !+ %$7#ci)* !+ *it(it$ !+%$!i$ "7 1; 7" "."(ici)* !+ #* c$7$( ($$ 7"!(i77$ i*!ic" #* (+%#7t"!$ .$%iti$&

F& P(#+" !+ P"#7,& E7 >ci!$ %#7="*7ic$ !i"3$ti3"!$ %+ #*+ c$* 7"% "-i*"%; =+*$7+% +i-i!"3$7+% ."(" !"( c$-.#+%t$% AO =#+(t+-+*t+ c$7$(+"!$%& L$% c$-.#+%t$%!i"3$*i$ %+ =$(-"* *ic"-+*t+ +* +7 =($; "% 4#+ 7"% %$7#ci$*+% !++* +*=(i"(%+ +*

i+7$ "*t+% !+ 7" !i"3$ti3"ci)*& CONFIRMAR SI EISTE EL REACTIVO EN ELLABORATORIO

P($c+!i-i+*t$ -+3c7+ 1 -L !+7 (+"cti$ !+ P"#7, c$* 2 -L !+ 7" %$7#ci)* !+ "-i*$>ci!$:7ici*"; ti($%i*"; t(i.t)="*$ +*=(+ +* "$ !+ i+7$; ":(+:#+ 1 -L !+ %$7#ci)* !+ *it(it$ !+%$!i$ N"NO2 "7 5 , !++ "7 =($ .$( / -i*#t$%& A7c"7i*ic+ 7" %$7#ci)* ""!i+*!$ 2 -L !+%$7#ci)* !+ c"($*"t$ !+ %$!i$ N"2CO/ , "*$t+ 7$% c$7$(+ =$(-"!$%&

G& P(#+" !+7 *it($.(#%i"t$& L$% :(#.$% ti$7+% (+"cci$*"* c$* *it($.(#%i"t$ !+ %$!i$ +*.(+%+*ci" !+ #* +'c+%$ !+ "-$*i"c$ ."(" !"( #* c$7$( ($$&

P($c+!i-i+*t$ -+3c7+ 0&5 -L !+ %$7#ci)* =(+%c" !+ *it($.(#%i"t$ !+ %$!i$ c$* 2 -L !+

%$7#ci)* !+ "-i*$>ci!$ "3#=("!$ ci%t+i*"; ci%ti*" , -+ti$*i*" +* i!()'i!$ !+ "-$*i$&

INTRUCCIONE PREVIA OBRE EGURIDAD

• TENER EN CUENTA LAS NORMAS DE SEGURIDAD AL UTILIARCADA UNO DE LOS REACTIVOS; PUESTO QUE ESTOS ESTANPREPARADOS CON COMPUESTOS ORGANICOS CADA UNO CONDIFERENTES GRADOS DE TOICIDAD; ACIDE; ETC&

• EN CASO DE ALGUNA QUEMADURA LAVARSE CON SUFICIENT E AGUA&

TABLA DE DATO:



M#+%t(" 1 ; < / = >

1 & Ni *i !( i*"2 & "* t$.($t?i c"/& Bi#(+t & H$.Yi*% c$7+5& Mi7 7)*6& P"#7,8 & N it ($.(#% i" t$


• HACER UNA TABLA CON LAS DIFERENTES PRUEBAS ANOTAR SIDIO POSITIVA O NEGATIVA EL COLOR OBSERVADO

• RESPUESTAS A LAS PREGUNTAS PLANTEADAS&

BIBLIOGRA48 A A CONULTAR O DE APO)O :

AMARIS A&; R$+(t$ , LOPERA P&; Pi+!"!& P(>ctic"% !+ i$4#-ic"& U*i+(%i!"! !+ A*ti$4#i" M+!+77*; 1<<5&

DE LA TORRE ARAMILLO; G+(->* , MORENO VESGA; P+!($& Q#-ic" O(:>*ic"&V$7& 2 U*i%#(& S"*t"=? !+ B$:$t>& 1<<5&

PLUMMER; D"! T& Bi$4#-ic" .(>ctic" 8[ E!ici)*& B$:$t>& E!it$(i"7 McG("J Hi77L"ti*$"-+(ic"*" S&A& 1<91&




PRACTICA NUMERO: =

NOMBRE DE LA PRACTICA DESNATURALIACIWN PROT EICAOBJETIVO GENERAL I!+*ti= ic"( "7:#*$% -"t+(i"7+% .$t+*ci"7-+*t+ !+%*"t#("7 i3"*t+% !+.($t+*"%; (+%.+ct$ " -"t+(i"% .(i-"% (ic"% +* .($t+*"% !+ =(+c#+*t+ #%$

+* 7" i*!#%t(i"&PROCEDIMIENTO



CALOR c" 7 i+*t+ !$% -L !+ c 7"(" !+ #+$ % i* ! i 7# i ( -+!i"*t+ #* "$-"( " +* t+-.+(" t# ("% !+ 90<0 $C& c$-."(+ c$* #*" -#+%t (" % i*c"7+*t"(&

ALCOHOL A !$% -L !+ c7"(" !+ #+$ %i* !i 7#i( ":(+:#+ !$% -L !+"7c$$7 i*!#%t(i"7 -+3c7+ , $%+(+&R+.it" +7 "*t+(i$( .($c+!i-i+*t$ c$* c"%+*" "7 1&

METALES PESADOS P(+."(+ t(+% t#$% !+ +*%",$@ c"!" #*$ c$* 1 -L !+c7"(" !+ #+$ !i 7#i!" +* #* .$c$ !+ ":#"& A7 *#-+($ 1 "!ici$*+ 5 :$t"% !+ *it("t$ !+ .7"t" 0&5 A7 *#-+($ 2 "!ici$*+ 5 :$t"% !+ c7$(#($ !+ -+(c#(i$ 0& 5 A7 *#-+($ / "!ici$*+ 5 :$t"% !+ "c+t"t$ !+ .7$-$ 0&5R+.it" +7 "*t+(i$( .($c+!i-i+*t$ c$* c"%+*" "7 1&

ACIDOS ORG ÁNICOS FUERTES A 1 -L !+ c7"(" !+ #+$ !i 7#i!" "!ici$*+1 -L !+ >ci!$ .c(ic$ "7 1&

R+.it" +7 .($c+!i-i+*t$ "*t+(i$(; "!ici$*"*!$ 1 -L !+ >ci!$ t( ic7$($"c?tic$"7 1R+.it" c$* c"%+*" "7 1&

ACIDOS INORGÁNICOS FUERTES A 1 -L !+ c7"(" !+ #+$ !i 7#i!""!ic i$*+ 1 -L !+ >c i!$ .+(c 7)( ic$ " 7 1 $ >c i!$ * t ( ic$ " 7 1 (+. i t" c$*c"%+*"&INTRUCCIONE PREVIA OBRE EGURIDAD:T$!$% 7$% (+"cti"% $= (+c+* "7t$% :("!$% !+ .+7i:($%i!"! .$( i*:+%ti)* $c$*t"ct$ c$* 7" .i+7; -"*i.#7"( c$* .i.+t" , .+(" !+ %#cci)*&CONTENIDO 7UE DEBE TENER EL IN4ORME: A*>7i% i% !+ (+%#7t"!$%; t"7" !+ !"t$% , %$7#ci)* "7 c#+%ti$*"(i$ .7"*t+"!$&

o Q#? +% !+%*"t#("7 i3"ci)* !+ #*" .($t+*"Xo Q#? +% c$":#7"ci)* !+ #*" .($t+*"Xo Q#? ="ct$(+% .($!#c+* 7" c$":#7"ci)* , 7" !+%*"t#("7 i3"ci)* !+ #*"

.($t+*"XBIBLIOGRA4I A A CONULTAR O DE APO)O D&LORIENT& Bi$4#-ic" A:($i*!#%t(i"7& Ac(ii"& 1<<6VARGAS;ENCESLAO& F#*!"-+*t$% !+ Ci+*ci" A7i-+*t"(i"PLUMMER; D"i!; Bi$4#-ic" P("ctic"; / +!& E!it$(i"7 McG("J Hi7 7L"ti*$"-+(ic"*"; S&A& B$:$t>& 1<9<&




PRACTICA NUMERO: >

NOMBRE DE LA PRACTICA

QUÍMICA DE LÍPIDOSOBJETIVO GENERAL



R+"7i3"( !i=+(+*t+% .(#+"% " 7 .i!$% 4#+ *$% !+t+(- i*+* %#% .($.i+!"!+%4#-ic"%&Mar-! Re,ere-ial

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PROCEDIMIENTO

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• Pu%! "e ?u!.

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• Prue&a "e ,r$!.

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• 4!#,!l$3i"!# (De#+!a"!'

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• Prue&a "e #!lu&il i"a".

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MATERIALE PARA TRAER• M"*t+4#i7 7"

• M"(:"(i*"

• 100 -L !+ "c+it+ +:+t"7 c $-+(ci"7

• 10 : !+ %+$

• Ac+it+ +:+t"7 c(#!$


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MATERIA BIOQUÍMICAPRACTICA NUMERO: @NOMBRE DE LA PRACTICA E-#7%i$*+% , +-#7:+*t+%; .(+."("ci)* !+-",$*+%"&OBJETIVO GENERAL D+=i*i( #*" +-#7%i)* ti.$ $J , J$P(+."("( +-#7%i$*+% #ti 7 i3"*!$ !i=+(+*t+% +-#7:+*t+%V+(i=ic"( +7 +=+ct$ !+7 -+!i$ >ci!$; >%ic$ , 7" "cci)* !+ ":#"% !#("% %$(+#*" +-#7%i)*&PROCEDIMIENTO

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BIBLIOGRA4I A A CONULTAR O DE APO)O VARGAS; +*c+%7"$& F#*!"-+*t$% !+ ci+*ci" "7 i-+*t"(i"; U*i+(%i!"!N"ci$*"7 !+ C$7$-i"&FENNEMA; OJ+* R& Q#-ic" !+ 7$% "7 i-+*t$%& E!& Ac(ii"& "(":$3";E%.""&



MATERIA BIOQUÍMICAPRACTICA NUMERO: NOMBRE DE LA PRÁCTICA ETRACCIWN DE BROMELINA PAPAINA; EIDENTIFICACIWN DE SU ACCIWN ENIMÁTICA&OBJETIVO GENERAL E=+ct#"( 7" +'t("cci)* !+ +*3i-"% !+ $(i:+* +:+t"7

."(" i!+*ti= ic"( %# "cci)* +*3i->tic"&PROCEDIMIENTO ETRACCIWN DE PAPAINA R+" 7i 3" ( c$( t+% " t ("?% !+ t$!" 7" %#.+( =i ci+ !+ #*" ."."," +(!+ ;(+c$:i+*!$ t$!$ +7 7>t+' 4#+ %"7+ !+ +%t" $.+("ci)*&

R+"7i3"( .(#+"% !+ %$7#i7 i!"! +* / t#$% !+ +*%",$; c$* ":#" !+%ti 7"!";+t"*$7 , H+'"*$; #ti 7 i3"*!$ #*"% :$t"% !+7 .($!#ct$ +* c"!" t#$&

ETRACCIWN DE BROMELINA

E't("+( +7 c$("3)* !+ #*" .i" +* +%t"!$ !+ -"!#("ci)* ]&



L ic#"( + 7 c$("3)* , (+" 7i 3" ( . (#+"% !+ %$7# i7 i!"! c$-$ +* + 7 c"%$"*t+(i$(&

EFECTO ENIMATICO&

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MATERIAL A TRAER

CARNECORAWN DE PI^ALÁTE DE PAPAA VERDE ABLANDADOR COMERCIALPLANCHA PASA ASAR

INTRUCCIONE PREVIA OBRE EGURIDAD:R+c$(!"( 4#+ *$ %+ !++ c$-+( +* +7 7"$("t$(i$&T+*+( 7"% .(+c"#ci$*+% i%t"% ."(" +7 -"*+$ !+ % $7+*t+% $(:>*ic$%&CONTENIDO 7UE DEBE TENER EL IN4ORME: A*>7i% i% !+ (+%#7t"!$%&D+t+(-i*"ci)* !+ 7" (+"cci)* +*3i->tic" 4#+ $c#((i) +* 7"$("t$(i$&

BIBLIOGRA4I A A CONULTAR O DE APO)O D&LORIENT& Bi$4#-ic" A:($i*!#%t(i"7& Ac(ii"& 1<<6

VARGAS; +*c+%7"$& F#*!"-+*t$% !+ Ci+*ci" A7i-+*t"(i"PLUMMER; D"i!; Bi$4#-ic" P("ctic"; / +!& E!it$(i"7 McG("J Hi7 7L"ti*$"-+(ic"*"; S&A& B$:$t>& 1<9<&




PRACTICA NUMERO: 1



NOMBRE DE LA PRACTICA PARDEAMIENTO ENIMÁT ICO&OBJETIVO GENERAL I!+*ti= ic"( 7$% !i=+(+*t+% ="ct$(+% 4#+ .($!#c+* +7 P"(!+"-i+*t$

+*3i->tic$; , 7$% !i=+(+*t+% -+c"*i%-$% !+ c$*t($7 !+ +%t+ =+*)-+*$i$4#-ic$&PROCEDIMIENTO

C$*t"ct$ !+7 "i(+ c$* +7 t+ i!$ +:+t"7S+7+cci$*+ #*" #*i!"! =(+%c"; %"*" , *$ -#, -"!#(" !+ !i+(%"%= (# t"% , $( t" 7i 3"%; $ " 7> t$!"% !+ .#7." 7"*c"; t"7 +% c$-$-"*3"*"; .+("; ."."; ,#c"; .7>t"*$ +(!+; "*"*$; :#","" 7"*c";7>+7" +* ":#" 7 i-.i" , %?4#+7"; %i* +%t($.+"(7"%&

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