Download - Dna y expresión genética
![Page 1: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/1.jpg)
Bio1112009
DNA y Expresión Genética
![Page 2: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/2.jpg)
DNAMolécula con información genética
Dirige la síntesis de cadena polipéptida
Autoreplicable
![Page 3: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/3.jpg)
![Page 4: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/4.jpg)
Estructura del DNANucleótidos
Bases nitrogenadas purinas piridiminasPentosa- desoxiribosaGrupo fosfato
![Page 5: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/5.jpg)
![Page 6: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/6.jpg)
![Page 7: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/7.jpg)
![Page 8: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/8.jpg)
Thymine
AdenineNucleotide
Cytosine
Guanine
Deoxyribose(sugar)
Phosphodiesterlinkage
Phosphategroup
![Page 9: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/9.jpg)
(a) Adenine Thymine
Deoxyribose Deoxyribose
DeoxyriboseDeoxyribose
Guanine Cytosine
![Page 10: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/10.jpg)
![Page 11: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/11.jpg)
2.0 nm
Majorgroove
Minorgroove
3.4nm
Sugar-phosphatebackbone
0.34 nm
= hydrogen
= atoms in base pairs
= phosphorus
= oxygen
= carbon
![Page 12: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/12.jpg)
![Page 13: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/13.jpg)
Replicación semiconservativa
![Page 14: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/14.jpg)
![Page 15: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/15.jpg)
Figure 11-10Page 228
DNA polymerase
DNA polymerase Origin of replication
Single-strandedbinding proteins
Direction ofreplicationRNA primer
DNAhelicase
Twist introduced intothe helix by unwinding
3’5’
3’5’
3’5’
3’5’
3’5’
3’5’
3’5’
3’5’
3’3’
![Page 16: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/16.jpg)
![Page 17: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/17.jpg)
3’
Leading strand
RNA primer
DNA polymeraseReplication fork
Lagging strand(first Okazaki fragment) Direction of
replication
DNA helix
5’
3’5’
3’5’
3’5’
![Page 18: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/18.jpg)
Leading strand
DNA ligase Third Okazaki fragment
Direction ofreplication
3’
5’
3’5’
3’5’
3’5’
3’ 5’
Lagging strand
![Page 19: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/19.jpg)
Leading strand
DNA ligase Third Okazaki fragment
Direction ofreplication
3’
5’
3’5’
3’5’
3’5’
3’ 5’
Lagging strand
![Page 20: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/20.jpg)
![Page 21: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/21.jpg)
![Page 22: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/22.jpg)
![Page 23: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/23.jpg)
![Page 24: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/24.jpg)
![Page 25: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/25.jpg)
![Page 26: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/26.jpg)
Transcripción
![Page 27: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/27.jpg)
![Page 28: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/28.jpg)
![Page 29: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/29.jpg)
![Page 30: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/30.jpg)
![Page 31: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/31.jpg)
![Page 32: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/32.jpg)
![Page 33: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/33.jpg)
Traducción
![Page 34: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/34.jpg)
![Page 35: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/35.jpg)
Translation
Codon 1 Codon 2 Codon 3Codon 4 Codon 5Codon 6
Polypeptide
Nontemplate strand
TranscriptionDNA
Template strand
mRNA(complementarycopy of templateDNA strand)
![Page 36: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/36.jpg)
![Page 37: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/37.jpg)
Second letter
U C A GF
irst
lett
er (
5’ e
nd)
U
C
A
G
Thi
rd le
tter
(3’
end
)
UCAG
UCAG
UCAG
UCAG
= Stop Codon
= Start Codon
![Page 38: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/38.jpg)
Promoter region Transcribed region
mRNA terminationsequence
Upstreamleadersequences
Protein-coding sequencesTranslated region
Downstreamtrail ingsequences
Start codon Stop codon – OH3’ end
Polypeptide
5’
3’5’
3’
5’ end
mRNA
![Page 39: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/39.jpg)
Leadersequence
mRNA
Smallribosomalsubunit
Start codon
Init iat ion factor
5’ 3’
![Page 40: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/40.jpg)
fMet
InitiatortRNA
3’ 5’
5’ 3’
![Page 41: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/41.jpg)
fMetP site
E site
Init iation complex
A site
Largeribosomalsubunit
5’ 3’
![Page 42: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/42.jpg)
Promoter
TemplateDNA strand
7-methylguanosine cap
1stexon
1stintron
2ndexon
2ndintron
3rdexon
mRNA terminationsequence
Transcription , capping of 5’ end
Stop codonStart codon
5’ end
(continue to next sl ide)
![Page 43: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/43.jpg)
(continue to next sl ide)
–AAA...Poly-A tail3’ end
5' end
1stexon
2ndexon
3rdexon
Protein-coding region
![Page 44: Dna y expresión genética](https://reader034.vdocumento.com/reader034/viewer/2022042614/5597f9251a28ab98608b488a/html5/thumbnails/44.jpg)
Nuclear pore
Transport through nuclearenvelope to cytosol
Nuclear envelope
Cytosol
5' endLeader Start codon Stop codon
–AAA...Poly-A tai l3' end