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Microorganismo Saccharomyces Cerevisiae Marca Lefersa Medio de Cultivo Componente Concentración [g/L] Peptona 10 Extracto de levadura 5 Glucosa riable (entre 0,1 g/L y 7 g/L)

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Page 1: Buena buena

Microorganismo Saccharomyces CerevisiaeMarca Lefersa

Medio de CultivoComponente Concentración [g/L]

Peptona 10Extracto de levadura 5

Glucosa Variable (entre 0,1 g/L y 7 g/L)

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7 g/L de glucosaTiempo [min] Tiempo [seg] Abs 600 nm Abs 505 nm Estándar 0.306 X [g/mL]

0 0 0.170 3.364 4.8359.E-0415 900 0.166 3.164 4.7197.E-0430 1800 0.151 2.912 4.2843.E-0445 2700 0.170 2.792 4.8359.E-0460 3600 0.175 3.170 4.9810.E-0475 4500 0.202 3.288 5.7647.E-0490 5400 0.239 2.620 6.8387.E-04

105 6300 0.275 2.690 7.8837.E-04120 7200 0.310 2.942 8.8996.E-04135 8100 0.347 2.510 9.9736.E-04150 9000 0.386 3.066 1.1106.E-03165 9900 0.455 2.248 1.3108.E-03180 10800 0.498 - 1.4357.E-03195 11700 0.593 - 1.7114.E-03210 12600 0.635 - 1.8333.E-03

4 g/L de glucosaTiempo [min] Tiempo [s] Abs 600 nm Abs 505 nm Estándar 0.224 X [g/mL]

0 0 0.298 1.435 8.5513.E-0420 1200 0.285 1.303 8.1739.E-0440 2400 0.288 1.505 8.2610.E-0460 3600 0.279 1.420 7.9998.E-0480 4800 0.338 1.424 9.7123.E-04

100 6000 0.300 1.371 8.6093.E-04110 6600 0.341 1.368 9.7994.E-04120 7200 0.332 1.351 9.5382.E-04140 8400 0.351 1.283 1.0090.E-03160 9600 0.381 1.421 1.0960.E-03180 10800 0.331 1.339 9.5092.E-04195 11700 0.416 1.389 1.1976.E-03

1 g/L de glucosaTiempo [min] Tiempo [s] Abs 600 nm Abs 505 nm Estándar 0.339 X [g/mL]

0 0 0.096 0.663 2.6879.E-0420 1200 0.087 0.552 2.4266.E-0440 2400 0.093 0.691 2.6008.E-0460 3600 0.094 0.692 2.6298.E-0480 4800 0.192 0.653 5.4744.E-04

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100 6000 0.208 0.710 5.9389.E-04120 7200 0.233 0.622 6.6645.E-04140 8400 0.328 0.577 9.4221.E-04160 9600 0.381 0.598 1.0960.E-03180 10800 0.361 0.599 1.0380.E-03200 12000 0.412 0.583 1.1860.E-03235 14100 0.508 - 1.4647.E-03

0 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 0.004 0.00450

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

f(x) = 344.508053651865 x + 0.00339443022763986R² = 0.999518589777203

Column BLinear (Column B)

Peso Seco [g/mL]

Abso

rban

cia a

600

nm

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6 g/L de glucosaX/X0 ln(X/X0) µ9 [1/s] Tiempo [min] Tiempo [s] Abs 600 nm

1.0000.E+00 0 0 0 0.1779.7599.E-01 -0.024302533 20 1200 0.1418.8595.E-01 -0.121089818 40 2400 0.2281.0000.E+00 4.440892E-16 60 3600 0.2341.0300.E+00 0.029570457 80 4800 0.2611.1921.E+00 0.175697022 100 6000 0.2791.4142.E+00 0.346539722 120 7200 0.3801.6303.E+00 0.488734666 140 8400 0.4381.8403.E+00 0.609948237 160 9600 0.4302.0624.E+00 0.72388246 180 10800 0.4932.2965.E+00 0.831394327 200 12000 0.5882.7107.E+00 0.9972011032.9688.E+00 1.0881536253.5390.E+00 1.2638486113.7911.E+00 1.332660553

3 g/L de glucosaln(X/X0) µ6 [1/s] Tiempo [min] Tiempo [s] Abs 600 nm

0.0000.E+00 0 0 0.305-4.5131.E-02 15 900 0.319-3.4534.E-02 30 1800 0.318-6.6668.E-02 45 2700 0.3081.2732.E-01 60 3600 0.3036.7659.E-03 75 4500 0.3201.3624.E-01 90 5400 0.3551.0922.E-01 105 6300 0.5781.6543.E-01 120 7200 0.3462.4822.E-01 135 8100 0.4051.0617.E-01 150 9000 0.4443.3686.E-01 165 9900 0.496

180 10800 0.499195 11700 0.478225 13500 0.601

0,5 g/L de glucosaln(X/X0) Tiempo [min] Tiempo [s] Abs 600 nm

0.0000.E+00 0 0 0.174-1.0225.E-01 15 900 0.168-3.2934.E-02 30 1800 0.242-2.1835.E-02 45 2700 0.2557.1134.E-01 60 3600 0.254

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7.9277.E-01 75 4500 0.2649.0805.E-01 90 5400 0.2641.2543.E+00 105 6300 0.2871.4055.E+00 120 7200 0.2901.3511.E+00 135 8100 0.2311.4844.E+00 150 9000 0.2691.6955.E+00 165 9900 0.301

190 11400 0.309223 13380 0.360238 14280 0.391253 15180 0.401268 16080 0.407283 16980 0.477298 17880 0.454313 18780 0.471328 19680 0.475343 20580 0.478358 21480 0.500433 25980 0.536

0 0.0005 0.001 0.0015 0.002 0.0025 0.003 0.0035 0.004 0.00450

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

f(x) = 344.508053651865 x + 0.00339443022763986R² = 0.999518589777203

Column BLinear (Column B)

Peso Seco [g/mL]

Abso

rban

cia a

600

nm

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6 g/L de glucosaAbs 505 nm Estándar 0.371 X [g/mL] ln(X/Xo) µ8 [1/s]

3.264 5.0390.E-04 02.282 3.9941.E-04 -0.232402882.366 6.5194.E-04 0.257567242.190 6.6936.E-04 0.2839308062.144 7.4773.E-04 0.3946541922.116 7.9998.E-04 0.4621967371.928 1.0931.E-03 0.7744298141.816 1.2615.E-03 0.9176722662.032 1.2383.E-03 0.899093004

- 1.4211.E-03 1.036834929- 1.6969.E-03 1.21417405

3 g/L de glucosaAbs 505 nm Estándar 0.318 X [g/mL] ln(X/X0) µ5 [1/s]

1.135 8.7545.E-04 01.067 9.1608.E-04 0.0453739530.971 9.1318.E-04 0.0422003541.001 8.8415.E-04 0.0098978041.098 8.6964.E-04 -0.006653381.052 9.1899.E-04 0.0485375111.000 1.0206.E-03 0.153392531.168 1.6679.E-03 0.644572511.068 9.9446.E-04 0.127461950.955 1.1657.E-03 0.2863549320.948 1.2789.E-03 0.3790357981.009 1.4299.E-03 0.490595851.015 1.4386.E-03 0.4966675140.991 1.3776.E-03 0.453370708

- 1.7346.E-03 0.683819998

0,5 g/L de glucosaAbs 505 nm Estándar X [g/mL] ln(X/X0)

0.275

0.329

4.9520.E-04 00.277 4.7778.E-04 -0.035803350.219 6.9258.E-04 0.3354668750.262 7.3031.E-04 0.388518890.243 7.2741.E-04 0.384536407

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0.2530.329

7.5644.E-04 0.423665030.220 7.5644.E-04 0.423665030.225 8.2320.E-04 0.508243160.263 8.3191.E-04 0.518765880.234 6.6065.E-04 0.288268067

- 7.7095.E-04 0.442669783- 8.6384.E-04 0.556428668- 8.8706.E-04 0.582955422

0.212

0.451

1.0351.E-03 0.737293070.205 1.1251.E-03 0.8206522450.183 1.1541.E-03 0.846124840.167 1.1715.E-03 0.8611026530.159 1.3747.E-03 1.0210414490.127 1.3079.E-03 0.9712583930.133 1.3573.E-03 1.0082915950.164 1.3689.E-03 1.0168095340.098 1.3776.E-03 1.0231507090.085 1.4415.E-03 1.068463238

- 1.5460.E-03 1.138449038

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5 g/L de glucosaTiempo [min] Tiempo [s] Abs 600 nm Abs 505 nm Estándar 0.511 X [g/mL]

0 0 0.082 1.623 Estándar 0.687 2.2815.E-0415 900 0.086 1.800 2.3976.E-0430 1800 0.092 1.770 2.5718.E-0445 2700 0.096 1.710 2.6879.E-0460 3600 0.103 1.815 2.8911.E-0475 4500 0.130 1.825 3.6748.E-0490 5400 0.144 1.932 4.0812.E-04

120 7200 0.195 1.785 5.5615.E-04135 8100 0.219 1.997 6.2582.E-04150 9000 0.263 1.923 7.5353.E-04165 9900 0.320 1.935 9.1899.E-04180 10800 0.336 1.790 9.6543.E-04220 13200 0.473 - 1.3631.E-03

2 g/L de glucosaTiempo [min] Tiempo [s] Abs 600 nm Abs 505 nm Estándar 0.303 X [g/mL]

0 0 0.093 1.010 2.6008.E-0415 900 0.107 0.951 3.0072.E-0430 1800 0.110 0.897 3.0942.E-0445 2700 0.123 0.803 3.4716.E-0460 3600 0.137 0.787 3.8780.E-0475 4500 0.148 0.771 4.1973.E-0490 5400 0.154 0.760 4.3714.E-04

105 6300 0.179 0.761 5.0971.E-04120 7200 0.198 0.721 5.6486.E-04135 8100 0.225 0.701 6.4323.E-04150 9000 0.243 - 6.9548.E-04

0,1 g/L de glucosaTiempo [min] Tiempo [s] Abs 600 nm Abs 505 nm Estándar X [g/mL]

0 0 0.340 0.193

0.349

9.7704.E-0420 1200 0.180 0.348 5.1261.E-0440 2400 0.267 0.296 7.6514.E-0460 3600 0.255 0.254 7.3031.E-0480 4800 0.295 0.243 8.4642.E-04

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100 6000 0.319 0.1780.349

9.1608.E-04120 7200 0.371 0.203 1.0670.E-03140 8400 0.331 0.117 9.5092.E-04160 9600 0.321 - 9.2189.E-04190 11400 0.472 - 1.3602.E-03230 13800 0.497 0.024

0.459

1.4328.E-03250 15000 0.543 0.034 1.5663.E-03270 16200 0.571 0.039 1.6476.E-03290 17400 0.526 0.021 1.5169.E-03310 18600 0.556 0.022 1.6040.E-03330 19800 0.543 0.040 1.5663.E-03350 21000 0.597 0.011 1.7230.E-03370 22200 0.538 0.008 1.5518.E-03

PERIODO DE LATENCIA MUY LARGA PRODUCTO DE LA BAJA CONCENTRACIÓN DE GLUCOSA AL INICIO

Page 10: Buena buena

ln(X/Xo) µ7 [1/s]0

0.0496379810.1197601580.1639174420.2367904650.476660810.58154728

0.8910381661.00905314

1.1947702851.3932674481.4425688671.787509569

ln(X/X0) µ4 [1/s]0

0.145182010.1737281920.2887975220.3994949410.47861599

0.5192719950.6728533610.77559085

0.9055186350.983615546

ln(X/X0)0

-0.64500799-0.24446248-0.29105476-0.14351228

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-0.064419690.088100109-0.02710193-0.058102550.3308542440.3828301910.4719328430.5225216640.4399210710.4957391310.4719328430.56731041

0.462623522

PERIODO DE LATENCIA MUY LARGA PRODUCTO DE LA BAJA CONCENTRACIÓN DE GLUCOSA AL INICIO

Page 12: Buena buena

Tiempo [seg] ln(X/X0) µ exp9 [1/s] Tiempo [seg]3600 0.0295705 1.5237E-046300 0.48873478100 0.72388259900 0.9972011

Tiempo [seg] X [g/L] S g/L3600 0.4981 10.35947716300 0.7884 8.790849678100 0.9974 8.202614389900 1.3108 7.34640523

3000 4000 5000 6000 7000 8000 9000 10000 110000.0000000

0.2000000

0.4000000

0.6000000

0.8000000

1.0000000

1.2000000

f(x) = 0.000152370361134689 x − 0.502936097366194R² = 0.997248438555106

θ [s]

ln[X

/Xo]

3000 4000 5000 6000 7000 8000 9000 10000 110000.0000

2.0000

4.0000

6.0000

8.0000

10.0000

12.0000

X [g/L]S [g/L]

Axis Title

Axis

Title

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Tiempo [seg] Abs 505 nm ln(X/X0) Estándar 0.306 S mg/dL S g/L0 3.364 0.0000 1099.34641 10.9934641

900 3.164 -0.0243 1033.98693 10.33986931800 2.912 -0.1211 951.633987 9.516339872700 2.792 0.0000 912.418301 9.124183013600 3.170 0.0296 1035.94771 10.3594771 So4500 3.288 0.1757 1074.5098 10.7450985400 2.620 0.3465 856.20915 8.56209156300 2.690 0.4887 879.084967 8.790849677200 2.942 0.6099 961.437908 9.614379088100 2.510 0.7239 820.261438 8.202614389000 3.066 0.8314 1001.96078 10.01960789900 2.248 0.9972 734.640523 7.34640523 Sf

10800 - 1.0882 -11700 - 1.2638 -12600 - 1.3327 -

So 10.3595Sf 7.3464

Xo 0.4981Xf 1.3108

3000 4000 5000 6000 7000 8000 9000 10000 110000.0000

2.0000

4.0000

6.0000

8.0000

10.0000

12.0000

X [g/L]S [g/L]

Axis Title

Axis

Title

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X [g/mL] X [g/L]4.8359.E-04 4.8359.E-014.7197.E-04 4.7197.E-014.2843.E-04 4.2843.E-014.8359.E-04 4.8359.E-014.9810.E-04 4.9810.E-01 Xo5.7647.E-04 5.7647.E-016.8387.E-04 6.8387.E-017.8837.E-04 7.8837.E-018.8996.E-04 8.8996.E-019.9736.E-04 9.9736.E-011.1106.E-03 1.1106.E+001.3108.E-03 1.3108.E+00 Xf1.4357.E-03 1.4357.E+001.7114.E-03 1.7114.E+001.8333.E-03 1.8333.E+00

Y'x/s [g biomasa/g sustrato] 0.2697

Page 15: Buena buena

Tiempo [s] ln(X/Xo) µ exp8 [1/s]2400 0.2576 1.1523E-043600 0.28394800 0.39476000 0.46227200 0.77448400 0.9177

Tiempo [s] X S2400 0.65194044 6.377358493600 0.66935648 5.902964964800 0.74772866 5.778975746000 0.79997678 5.703504047200 1.09314679 5.19676558400 1.26150184 4.89487871

2000 3000 4000 5000 6000 7000 8000 90000.0000

0.1000

0.2000

0.3000

0.4000

0.5000

0.6000

0.7000

0.8000

0.9000

1.0000

f(x) = 0.00011522773089675 x − 0.107154571142971R² = 0.91322395786714

θ [s]

ln[X

/Xo]

2000 3000 4000 5000 6000 7000 8000 90000

1

2

3

4

5

6

7

Column DColumn E

Axis Title

Axis

Title

Page 16: Buena buena

Tiempo [s] Abs 505 nm ln(X/Xo) Estándar 0.371 S [mg/dL] S [g/L]0 3.264 0.0000 879.784367 8.79784367

1200 2.282 -0.2324 615.09434 6.15094342400 2.366 0.2576 637.735849 6.37735849 So3600 2.190 0.2839 590.296496 5.902964964800 2.144 0.3947 577.897574 5.778975746000 2.116 0.4622 570.350404 5.703504047200 1.928 0.7744 519.67655 5.19676558400 1.816 0.9177 489.487871 4.89487871 Sf9600 2.032 0.8991 547.708895 5.47708895

10800 - 1.036812000 - 1.2142

So 6.37735849 Y'x/s (exp 8)Sf 4.89487871

Xo 0.65194044Xf 1.26150184

2000 3000 4000 5000 6000 7000 8000 90000

1

2

3

4

5

6

7

Column DColumn E

Axis Title

Axis

Title

Page 17: Buena buena

X [g/mL] X [g/L]5.0390.E-04 5.0390.E-013.9941.E-04 3.9941.E-016.5194.E-04 6.5194.E-01 Xo6.6936.E-04 6.6936.E-017.4773.E-04 7.4773.E-017.9998.E-04 7.9998.E-011.0931.E-03 1.0931.E+001.2615.E-03 1.2615.E+00 Xf1.2383.E-03 1.2383.E+001.4211.E-03 1.4211.E+001.6969.E-03 1.6969.E+00

0.41117688

Page 18: Buena buena

Tiempo [s] ln(X/Xo) µ exp7 [1/s] Tiempo [min]2700 0.16391744 1.6585E-043600 0.236790474500 0.476660815400 0.581547287200 0.891038178100 1.009053149000 1.194770299900 1.39326745

10800 1.44256887

Tiempo [s] X S2700 0.26878755 2.854757933600 0.28910627 3.030050084500 0.36747845 3.046744575400 0.40811587 3.225375637200 0.55615222 2.979966618100 0.62581638 3.333889829000 0.753534 3.210350589900 0.91898639 3.23038397

10800 0.96542916 2.98831386

2000 3000 4000 5000 6000 7000 8000 9000 10000 11000 120000

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

f(x) = 0.000165852950101842 x − 0.306731849134733R² = 0.994140819336542

θ [s]

ln[X

/Xo]

20003000

40005000

60007000

80009000

1000011000

120000

0.5

1

1.5

2

2.5

3

3.5

4

Chart Title

Column DColumn E

Axis Title

Axis

Title

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Estándar 0.511 Est. PromTiempo [min] Tiempo [s] Abs 505 nm Estándar 0.687 0.599 S [mg/dL]

0 0 1.623 270.95158615 900 1.800 300.50083530 1800 1.770 295.49248745 2700 1.710 285.47579360 3600 1.815 303.00500875 4500 1.825 304.67445790 5400 1.932 322.537563

120 7200 1.785 297.996661135 8100 1.997 333.388982150 9000 1.923 321.035058165 9900 1.935 323.038397180 10800 1.790 298.831386220 13200 - #VALUE!

SoSf

XoXf

20003000

40005000

60007000

80009000

1000011000

120000

0.5

1

1.5

2

2.5

3

3.5

4

Chart Title

Column DColumn E

Axis Title

Axis

Title

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S [g/L] X [g/mL] X [g/L]2.70951586 2.2815.E-04 2.2815.E-013.00500835 2.3976.E-04 2.3976.E-012.95492487 2.5718.E-04 2.5718.E-012.85475793 So 2.6879.E-04 2.6879.E-01 Xo3.03005008 2.8911.E-04 2.8911.E-013.04674457 3.6748.E-04 3.6748.E-013.22537563 4.0812.E-04 4.0812.E-012.97996661 5.5615.E-04 5.5615.E-013.33388982 6.2582.E-04 6.2582.E-013.21035058 7.5353.E-04 7.5353.E-013.23038397 9.1899.E-04 9.1899.E-012.98831386 Sf 9.6543.E-04 9.6543.E-01 Xf

#VALUE! 1.3631.E-03 1.3631.E+00

2.85475793 Y'x/s -5.21612.98831386

0.268787550.96542916

Page 21: Buena buena

Tiempo [s] Abs 600 nm ln(X/X0) Abs 505 nm6000 0.3 0.00676593 1.3716600 0.341 0.13624326 1.3687200 0.332 0.1092227 1.3518400 0.351 0.16543389 1.2839600 0.381 0.24821693 1.421

11700 0.416 0.3368601 1.389

Tiempo [s] ln(X/X0) µ exp6 [1/s]6000 0.0068 6.6112E-057200 0.10928400 0.1654

Tiempo [s] X S6000 0.86093292 6.120535717200 0.95381847 6.031258400 1.00896926 5.72767857

5000 6000 7000 8000 9000 10000 11000 12000 130000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

f(x) = 5.15735244649991E-05 x − 0.258357776476683R² = 0.92042614115803

Chart Title

Axis Title

Axis

Title

5500 6000 6500 7000 7500 8000 8500 90000.0000

0.0200

0.0400

0.0600

0.0800

0.1000

0.1200

0.1400

0.1600

0.1800

f(x) = 6.61116515364188E-05 x − 0.382196385524666R² = 0.972463164975103

θ [s]

ln[X

/Xo]

5500 6000 6500 7000 7500 8000 8500 90000

1

2

3

4

5

6

7

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 22: Buena buena

5500 6000 6500 7000 7500 8000 8500 90000

1

2

3

4

5

6

7

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 23: Buena buena

Tiempo [min] Tiempo [s] Abs 505 nm Estándar 0.224 S [mg/dL] S [g/L]0 0 1.435 640.625 6.40625

20 1200 1.303 581.696429 5.8169642940 2400 1.505 671.875 6.7187560 3600 1.420 633.928571 6.3392857180 4800 1.424 635.714286 6.35714286

100 6000 1.371 612.053571 6.12053571110 6600 1.368 610.714286 6.10714286120 7200 1.351 603.125 6.03125140 8400 1.283 572.767857 5.72767857160 9600 1.421 634.375 6.34375180 10800 1.339 597.767857 5.97767857195 11700 1.389 620.089286 6.20089286

So 6.12053571Sf 5.72767857

Xo 0.86093292Xf 1.00896926

5000 6000 7000 8000 9000 10000 11000 12000 130000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

f(x) = 5.15735244649991E-05 x − 0.258357776476683R² = 0.92042614115803

Chart Title

Axis Title

Axis

Title

5500 6000 6500 7000 7500 8000 8500 90000

1

2

3

4

5

6

7

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 24: Buena buena

5500 6000 6500 7000 7500 8000 8500 90000

1

2

3

4

5

6

7

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 25: Buena buena

X [g/mL] X [g/L]8.5513.E-04 8.5513.E-018.1739.E-04 8.1739.E-018.2610.E-04 8.2610.E-017.9998.E-04 7.9998.E-019.7123.E-04 9.7123.E-01

So 8.6093.E-04 8.6093.E-01 Xo9.7994.E-04 9.7994.E-019.5382.E-04 9.5382.E-01

Sf 1.0090.E-03 1.0090.E+00 Xf1.0960.E-03 1.0960.E+009.5092.E-04 9.5092.E-011.1976.E-03 1.1976.E+00

Y'x/s 0.3768

Page 26: Buena buena

t ln µ exp5 [1/s]4500 0.04853751 6.7039E-055400 0.153392538100 0.286354939000 0.3790358

t [s] X S4500 0.91898639 3.30817615400 1.02057995 3.144654098100 1.16571362 3.003144659000 1.27891788 2.98113208

4000 5000 6000 7000 8000 9000 100000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

f(x) = 6.70385177224231E-05 x − 0.235679801759233R² = 0.974697418688333

θ [s]

ln[X

/Xo]

4000 5000 6000 7000 8000 9000 100000

0.5

1

1.5

2

2.5

3

3.5

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 27: Buena buena

Tiempo [min] Tiempo [s] Abs 505 nm Estándar 0.3180 0 1.135

15 900 1.06730 1800 0.97145 2700 1.00160 3600 1.09875 4500 1.05290 5400 1.000

105 6300 1.168120 7200 1.068135 8100 0.955150 9000 0.948165 9900 1.009180 10800 1.015195 11700 0.991225 13500 -

4000 5000 6000 7000 8000 9000 100000

0.05

0.1

0.15

0.2

0.25

0.3

0.35

0.4

f(x) = 6.70385177224231E-05 x − 0.235679801759233R² = 0.974697418688333

θ [s]

ln[X

/Xo]

4000 5000 6000 7000 8000 9000 100000

0.5

1

1.5

2

2.5

3

3.5

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 28: Buena buena

S [mg/dL] S [g/L] X [g/mL] X [g/L]356.918239 3.56918239 8.7545.E-04 0.87544629335.534591 3.35534591 9.1608.E-04 0.91608371305.345912 3.05345912 9.1318.E-04 0.91318104314.779874 3.14779874 8.8415.E-04 0.88415431345.283019 3.45283019 8.6964.E-04 0.86964094

330.81761 3.3081761 So 9.1899.E-04 0.91898639 Xo314.465409 3.14465409 1.0206.E-03 1.02057995367.295597 3.67295597 1.6679.E-03 1.66787611335.849057 3.35849057 9.9446.E-04 0.99445589300.314465 3.00314465 1.1657.E-03 1.16571362298.113208 2.98113208 Sf 1.2789.E-03 1.27891788 Xf317.295597 3.17295597 1.4299.E-03 1.4298569

319.18239 3.1918239 1.4386.E-03 1.43856492311.63522 3.1163522 1.3776.E-03 1.37760878

#VALUE! #VALUE! 1.7346.E-03 1.7346376

So 3.3081761 Y'x/s 1.1006Sf 2.98113208

Xo 0.91898639Xf 1.27891788

Page 29: Buena buena

Tiempo [s] ln(X/X0) µ exp4 [1/s] Tiempo [min]2700 0.28879752 1.1118E-04 03600 0.39949494 154500 0.47861599 305400 0.519272 457200 0.77559085 608100 0.90551863 75

90105120135150

Tiempo [s] X S2700 0.34715973 2.650165023600 0.38779716 2.597359744500 0.41972657 2.544554465400 0.43714261 2.508250837200 0.56486024 2.379537958100 0.64323242 2.31353135

2000 3000 4000 5000 6000 7000 8000 90000

0.1

0.2

0.3

0.4

0.5

0.6

0.7

0.8

0.9

1

f(x) = 0.000111178225936492 x − 0.0224706974250368R² = 0.982479791282189

θ [s]

ln[X

/Xo]

2000 3000 4000 5000 6000 7000 8000 90000

0.5

1

1.5

2

2.5

3

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 30: Buena buena

Tiempo [s] Abs 505 nm Estándar 0.303 S [mg/dL] S [g/L] X [g/mL]0 1.010 333.333333 3.33333333 2.6008.E-04

900 0.951 313.861386 3.13861386 3.0072.E-041800 0.897 296.039604 2.96039604 3.0942.E-042700 0.803 265.016502 2.65016502 So 3.4716.E-043600 0.787 259.735974 2.59735974 3.8780.E-044500 0.771 254.455446 2.54455446 4.1973.E-045400 0.760 250.825083 2.50825083 4.3714.E-046300 0.761 251.155116 2.51155116 5.0971.E-047200 0.721 237.953795 2.37953795 5.6486.E-048100 0.701 231.353135 2.31353135 Sf 6.4323.E-049000 - #VALUE! 6.9548.E-04

So 2.65016502 Y'x/sSf 2.31353135

Xo 0.34715973Xf 0.64323242

2000 3000 4000 5000 6000 7000 8000 90000

0.5

1

1.5

2

2.5

3

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 31: Buena buena

X [g/L]0.260079530.300716960.309424980.34715973 Xo0.387797160.419726570.437142610.509709440.564860240.64323242 Xf0.69548054

0.8795

Page 32: Buena buena

Tiempo [s] ln(X/X0) µ exp3 [1/s] Tiempo [min]4800 0.7113 1.1737E-04 07200 0.9080 209600 1.4055 40

12000 1.4844 6080

100120140160180200235

Tiempo [s] X S4800 0.5474442 1.926253697200 0.6664538 1.834808269600 1.09604946 1.7640118

12000 1.18603234 1.71976401

4000 5000 6000 7000 8000 9000 10000 11000 12000 130000.0000

0.2000

0.4000

0.6000

0.8000

1.0000

1.2000

1.4000

1.6000f(x) = 0.000117367571099101 x + 0.141458048940326R² = 0.931125634927679

θ [s]

ln[X

/Xo]

4000 5000 6000 7000 8000 9000 10000 11000 12000 130000

0.5

1

1.5

2

2.5

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 33: Buena buena

Tiempo [s] Abs 505 nm Estándar 0.339 S [mg/dL] S [g/L] X [g/mL]0 0.663 195.575221 1.95575221 2.6879.E-04

1200 0.552 162.831858 1.62831858 2.4266.E-042400 0.691 203.834808 2.03834808 2.6008.E-043600 0.692 204.129794 2.04129794 2.6298.E-044800 0.653 192.625369 1.92625369 So 5.4744.E-046000 0.710 209.439528 2.09439528 5.9389.E-047200 0.622 183.480826 1.83480826 6.6645.E-048400 0.577 170.20649 1.7020649 9.4221.E-049600 0.598 176.40118 1.7640118 1.0960.E-03

10800 0.599 176.696165 1.76696165 1.0380.E-0312000 0.583 171.976401 1.71976401 Sf 1.1860.E-0314100 - #VALUE! #VALUE! 1.4647.E-03

So 1.92625369 Y'x/sSf 1.71976401

Xo 0.5474442Xf 1.18603234

4000 5000 6000 7000 8000 9000 10000 11000 12000 130000

0.5

1

1.5

2

2.5

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 34: Buena buena

X [g/L]0.268787550.242663490.260079530.26298221

0.5474442 Xo0.59388697

0.66645380.942207771.096049461.037995991.18603234 Xf1.46468898

3.0926

Page 35: Buena buena

Tiempo [s] ln(X/X0) µ exp2 [1/s]2700 0.3885 3.8113E-054500 0.4237

13380 0.737314280 0.820715180 0.846116080 0.861116980 1.021017880 0.971320580 1.023221480 1.0685

Tiempo [s] X S2700 0.73031262 0.796352584500 0.75643668 0.76899696

13380 1.03509332 0.4700665214280 1.1250762 0.4545454515180 1.15410293 0.4057649716080 1.17151897 0.3702882516980 1.3747061 0.3525498917880 1.30794462 0.2815964520580 1.37760878 0.217294921480 1.44146759 0.18847007

0 5000 10000 15000 20000 250000.0000

0.2000

0.4000

0.6000

0.8000

1.0000

1.2000

f(x) = 3.81134408377824E-05 x + 0.270952393885604R² = 0.970192084825103

θ [s]

ln[X

/Xo]

0 5000 10000 15000 20000 250000

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 36: Buena buena

Tiempo [min] Tiempo [s] Abs 505 nm Estándar S [mg/dL]0 0 0.275

0.329

83.586626115 900 0.277 84.194528930 1800 0.219 66.565349545 2700 0.262 79.635258460 3600 0.243 73.860182475 4500 0.253 76.89969690 5400 0.220 66.8693009

105 6300 0.225 68.3890578120 7200 0.263 79.9392097

* 135 8100 0.234 71.1246201150 9000 - #VALUE!165 9900 - #VALUE!190 11400 - #VALUE!223 13380 0.212

0.451

47.0066519238 14280 0.205 45.4545455253 15180 0.183 40.5764967268 16080 0.167 37.0288248283 16980 0.159 35.2549889298 17880 0.127 28.1596452313 18780 0.133 29.4900222328 19680 0.164 36.3636364343 20580 0.098 21.72949358 21480 0.085 18.8470067433 25980 - #VALUE!

SoSf

XoXf

0 5000 10000 15000 20000 250000

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

Chart Title

Column DColumn E

Axis Title

Axis

Title

Page 37: Buena buena

S [g/L] X [g/mL]0.83586626 4.9520.E-04 4.9520.E-010.84194529 4.7778.E-04 4.7778.E-01

0.6656535 6.9258.E-04 6.9258.E-010.79635258 So 7.3031.E-04 7.3031.E-01 Xo0.73860182 7.2741.E-04 7.2741.E-010.76899696 7.5644.E-04 7.5644.E-010.66869301 7.5644.E-04 7.5644.E-010.68389058 8.2320.E-04 8.2320.E-01

0.7993921 8.3191.E-04 8.3191.E-010.7112462 6.6065.E-04 6.6065.E-01

#VALUE! 7.7095.E-04 7.7095.E-01#VALUE! 8.6384.E-04 8.6384.E-01#VALUE! 8.8706.E-04 8.8706.E-01

0.47006652 1.0351.E-03 1.0351.E+000.45454545 1.1251.E-03 1.1251.E+000.40576497 1.1541.E-03 1.1541.E+000.37028825 1.1715.E-03 1.1715.E+000.35254989 1.3747.E-03 1.3747.E+000.28159645 1.3079.E-03 1.3079.E+000.29490022 1.3573.E-03 1.3573.E+000.36363636 1.3689.E-03 1.3689.E+00

0.2172949 1.3776.E-03 1.3776.E+000.18847007 Sf 1.4415.E-03 1.4415.E+00 Xf

#VALUE! 1.5460.E-03 1.5460.E+00

0.79635258 Y'x/s 1.16990.18847007

0.730312621.44146759

Page 38: Buena buena

Tiempo [s] ln(X/X0) µ exp1 [1/s]13800 0.38283019 2.5622E-0517400 0.4399210721000 0.56731041

Tiempo [s] X S13800 1.43275957 0.0522875817400 1.5169371 0.0457516321000 1.72302691 0.02396514

10000 12000 14000 16000 18000 20000 220000.3

0.35

0.4

0.45

0.5

0.55

0.6

f(x) = 2.56222526752767E-05 x + 0.0175266942037656R² = 0.953831841694961

θ [s]

ln[X

/Xo]

13000 14000 15000 16000 17000 18000 19000 20000 21000 220000

0.20.40.60.8

11.21.41.61.8

2

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 39: Buena buena

Tiempo [min] Tiempo [s] Abs 505 nm Estándar S [mg/dL]0 0 0.193

0.349

55.300859620 1200 0.348 99.71346740 2400 0.296 84.813753660 3600 0.254 72.779369680 4800 0.243 69.6275072

100 6000 0.178 51.0028653120 7200 0.203 58.1661891140 8400 0.117 33.5243553160 9600 - #VALUE!190 11400 - #VALUE!230 13800 0.024

0.459

5.22875817250 15000 0.034 7.40740741270 16200 0.039 8.49673203290 17400 0.021 4.5751634310 18600 0.022 4.79302832330 19800 0.040 8.71459695350 21000 0.011 2.39651416370 22200 0.008 1.74291939

SoSf

XoXf

10000 12000 14000 16000 18000 20000 220000.3

0.35

0.4

0.45

0.5

0.55

0.6

f(x) = 2.56222526752767E-05 x + 0.0175266942037656R² = 0.953831841694961

θ [s]

ln[X

/Xo]

13000 14000 15000 16000 17000 18000 19000 20000 21000 220000

0.20.40.60.8

11.21.41.61.8

2

Chart Title

X [g/L]S [g/L]

Axis Title

Axis

Title

Page 40: Buena buena

S [g/L] X [g/mL] X [g/L]0.5530086 0.00097704 0.97703985

0.99713467 0.00051261 0.512612120.84813754 0.00076514 0.7651447

0.7277937 0.00073031 0.730312620.69627507 0.00084642 0.846419550.51002865 0.00091608 0.916083710.58166189 0.00106702 1.067022730.33524355 0.00095092 0.95091579

#VALUE! 0.00092189 0.92188906#VALUE! 0.00136019 1.36019274

0.05228758 So 0.00143276 1.43275957 Xo0.07407407 0.00156628 1.566282550.08496732 0.00164756 1.64755740.04575163 0.00151694 1.51693710.04793028 0.00160402 1.60401730.08714597 0.00156628 1.566282550.02396514 Sf 0.00172303 1.72302691 Xf0.01742919 0.00155177 1.55176918

0.05228758 Y'x/s 10.24870.02396514

1.432759571.72302691

Page 41: Buena buena

Lineweaver-BurkeCorrida S [g/L] µ [1/s] 1/µ 1/S 1/µmáx Ks/µmáx

1 0.1 2.5622E-05 39028.9595 10.0000

9933.1 3062.9

2 0.5 3.8113E-05 26237.7666 2.00003 1 1.1737E-04 8520.0648 1.00004 2 1.1118E-04 8994.4235 0.50005 3 6.7039E-05 14916.6903 0.33336 4 6.6112E-05 15125.8470 0.25007 5 1.6585E-04 6029.5448 0.20008 6 1.1523E-04 8678.2956 0.16679 7 1.5237E-04 6562.9717 0.1429

Lineweaver-Burke

0 1 2 3 4 5 6 7 80.0000E+00

2.0000E-05

4.0000E-05

6.0000E-05

8.0000E-05

1.0000E-04

1.2000E-04

1.4000E-04

1.6000E-04

1.8000E-04

S [g/L]

µ [1

/s]

0.0000 2.0000 4.0000 6.0000 8.0000 10.0000 12.00000.0000

5000.0000

10000.0000

15000.0000

20000.0000

25000.0000

30000.0000

35000.0000

40000.0000

45000.0000

f(x) = 3062.9151018127 x + 9933.09791284925R² = 0.788955037292111

1/S [L/g]1/

µ [s

]

Page 42: Buena buena

Langmuirµmáx [1/s] Ks [g/L] S/µ S [g/L] 1/µmáx Ks/µmáx µmáx

1.0067E-04 0.3084

3.9029E+03 0.1

6731.9 9378.8 1.4855.E-04

1.3119E+04 0.58.5201E+03 11.7989E+04 24.4750E+04 36.0503E+04 43.0148E+04 55.2070E+04 64.5941E+04 7

Lineweaver-Burke Langmuir

0.0000 2.0000 4.0000 6.0000 8.0000 10.0000 12.00000.0000

5000.0000

10000.0000

15000.0000

20000.0000

25000.0000

30000.0000

35000.0000

40000.0000

45000.0000

f(x) = 3062.9151018127 x + 9933.09791284925R² = 0.788955037292111

1/S [L/g]

1/µ

[s]

0 1 2 3 4 5 6 7 80.0000E+00

1.0000E+04

2.0000E+04

3.0000E+04

4.0000E+04

5.0000E+04

6.0000E+04

7.0000E+04

f(x) = 6731.92563462125 x + 9378.81957435319R² = 0.647548643049034

S [g/L]

S/µ

[gS/

L]

Page 43: Buena buena

Eadie-HofsteeKs µ/S µ [1/s] Ks µmax

1.3932

2.5622E-04 2.5622E-05

3.2895E-01 1.1803E-04

7.6226E-05 3.8113E-051.1737E-04 1.1737E-045.5590E-05 1.1118E-042.2346E-05 6.7039E-051.6528E-05 6.6112E-053.3170E-05 1.6585E-041.9205E-05 1.1523E-042.1767E-05 1.5237E-04

Eadie-Hofstee

0 1 2 3 4 5 6 7 80.0000E+00

1.0000E+04

2.0000E+04

3.0000E+04

4.0000E+04

5.0000E+04

6.0000E+04

7.0000E+04

f(x) = 6731.92563462125 x + 9378.81957435319R² = 0.647548643049034

S [g/L]

S/µ

[gS/

L]

0.0000E+00

5.0000E-05

1.0000E-04

1.5000E-04

2.0000E-04

2.5000E-04

3.0000E-040.0000E+00

2.0000E-05

4.0000E-05

6.0000E-05

8.0000E-05

1.0000E-04

1.2000E-04

1.4000E-04

1.6000E-04

1.8000E-04

f(x) = − 0.32894691871865 x + 0.000118034907556579R² = 0.274357898222171

µ/S [g/(Ls)]

µ [1

/s]

Page 44: Buena buena

0.0000E+00

5.0000E-05

1.0000E-04

1.5000E-04

2.0000E-04

2.5000E-04

3.0000E-040.0000E+00

2.0000E-05

4.0000E-05

6.0000E-05

8.0000E-05

1.0000E-04

1.2000E-04

1.4000E-04

1.6000E-04

1.8000E-04

f(x) = − 0.32894691871865 x + 0.000118034907556579R² = 0.274357898222171

µ/S [g/(Ls)]

µ [1

/s]

Page 45: Buena buena

Peso seco [g/mL] ABS [600 nm]0 0

0.00032 0.1120.00064 0.2210.00128 0.4430.00193 0.6670.0025 0.8850.003 1.054

0.0033 1.1290.0037 1.2620.0038 1.320.0039 1.34

0 0.001 0.002 0.003 0.004 0.0050

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

f(x) = 344.508053651865 x + 0.00339443022763986R² = 0.999518589777203

Column BLinear (Column B)

Peso Seco [g/mL]

Abso

rban

cia a

600

nm

Page 46: Buena buena

0 0.001 0.002 0.003 0.004 0.0050

0.2

0.4

0.6

0.8

1

1.2

1.4

1.6

f(x) = 344.508053651865 x + 0.00339443022763986R² = 0.999518589777203

Column BLinear (Column B)

Peso Seco [g/mL]

Abso

rban

cia a

600

nm