pEASY-Blunt Simple Cloning Vector Complete sequence

AGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGAGCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGGAATTGTGAGCGGATAACAATTTCACACAGGAAACAGCTATGACCATGATTACGCCAAGCTGCCCTTAAGGGCAGCTTCAATTCGCCCTATAGTGAGTCGTATTACAATTCACTGGCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCCTTGCAGCACATCCCCCTTTCGCCAGCTGGCGTAATAGCGAAGAGGCCCGCACCGATCGCCCTTCCCAACAGTTGCGCAGCCTGAATGGCGAATGGACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATTCAGGGCGCAAGGGCTGCTAAAGGAAGCGGAACACGTAGAAAGCCAGTCCGCAGAAACGGTGCTGACCCCGGATGAATGTCAGCTACTGGGCTATCTGGACAAGGGAAAACGCAAGCGCAAAGAGAAAGCAGGTAGCTTGCAGTGGGCTTACATGGCGATAGCTAGACTGGGCGGTTTTATGGACAGCAAGCGAACCGGAATTGCCAGCTGGGGCGCCCTCTGGTAAGGTTGGGAAGCCCTGCAAAGTAAACTGGATGGCTTTCTTGCCGCCAAGGATCTGATGGCGCAGGGGATCAAGATCTGATCAAGAGACAGGATGAGGATCGTTTCGCATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCCCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCACGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGAATTGAAAAAGGAAGAGTATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGA

TCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAACTGTCAGACCAAGTTTACTCATATATACTTTAGATTGATTTAAAACTTCATTTTTAATTTAAAAGGATCTAGGTGAAGATCCTTTTTGATAATCTCATGACCAAAATCCCTTAACGTGAGTTTTCGTTCCACTGAGCGTCAGACCCCGTAGAAAAGATCAAAGGATCTTCTTGAGATCCTTTTTTTCTGCGCGTAATCTGCTGCTTGCAAACAAAAAAACCACCGCTACCAGCGGTGGTTTGTTTGCCGGATCAAGAGCTACCAACTCTTTTTCCGAAGGTAACTGGCTTCAGCAGAGCGCAGATACCAAATACTGTTCTTCTAGTGTAGCCGTAGTTAGGCCACCACTTCAAGAACTCTGTAGCACCGCCTACATACCTCGCTCTGCTAATCCTGTTACCAGTGGCTGCTGCCAGTGGCGATAAGTCGTGTCTTACCGGGTTGGACTCAAGACGATAGTTACCGGATAAGGCGCAGCGGTCGGGCTGAACGGGGGGTTCGTGCACACAGCCCAGCTTGGAGCGAACGACCTACACCGAACTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAG

M13F：5'-GTAAAACGACGGCCAGT-3' 目录号：GJ101-01M13R：5'-CAGGAAACAGCTATGAC-3' 目录号：GJ101-11LacZfragment: bases 1-449M13 reverse priming site: bases 205-221T7 promoter/priming site: bases 265-282M13 Forward (-20) priming site: bases 290-305f1 origin: bases 447-884Kanamycin resistance ORF: bases 1218-2012Ampicillin resistance ORF: bases 2030-2890pUC origin: bases 3035-3708

pEASY-Blunt Simple Cloning Vector Restriction Map

NEWMOLCutters

M.CviQII (349) rar 25, 46, 72, 123, 127, 145, 152, 159, 166, 201245, 256, 262, 289, 303, 344, 353, 369, 414, 514536, 540, 545, 549, 554, 558, 562, 571, 579, 593607, 611, 619, 625, 632, 643, 658, 675, 707, 716736, 751, 757, 775, 786, 795, 799, 804, 815, 824854, 865, 876, 887, 1024, 1035, 1063, 1106, 1147, 11511215, 1250, 1261, 1278, 1313, 1329, 1357, 1361, 1444, 14611524, 1539, 1543, 1596, 1617, 1657, 1674, 1713, 1731, 17401770, 1796, 1826, 1836, 1914, 1937, 1941, 1947, 1963, 19701985, 1995, 2007, 2040, 2047, 2060, 2064, 2068, 2072, 20812087, 2094, 2100, 2177, 2195, 2203, 2221, 2238, 2247,

22972317, 2329, 2341, 2363, 2434, 2438, 2475, 2479, 2505, 25102621, 2625, 2631, 2684, 2692, 2699, 2714, 2751, 2791, 28722907, 2911, 2921, 2932, 2939, 2944, 2951, 2975, 2987, 30033014, 3020, 3060, 3072, 3076, 3097, 3132, 3136, 3159, 31633181, 3210, 3241, 3250, 3272, 3276, 3326, 3340, 3391, 34093473, 3545, 3569, 3587, 3596, 3602, 3614, 3625, 3670, 36803688, 3699, 3705, 3713, 3717, 3736, 3760, 3780, 18, 8396, 116, 181, 188, 211, 237, 248, 275, 323, 392436, 443, 471, 590, 597, 652, 727, 761, 767, 809840, 847, 860, 873, 883, 897, 907, 911, 917, 926941, 962, 989, 993, 1001, 1007, 1012, 1076, 1080, 10861113, 1121, 1131, 1136, 1160, 1184, 1195, 1207, 1227, 1231

1269, 1368, 1391, 1395, 1407, 1476, 1483, 1506, 1551, 15551627, 1631, 1644, 1648, 1665, 1698, 1725, 1743, 1764, 18021815, 1908, 1923, 2004, 2014, 2019, 2023, 2027, 2035, 21082119, 2125, 2135, 2154, 2168, 2189, 2198, 2213, 2233, 22432289, 2323, 2336, 2354, 2383, 2387, 2413, 2454, 2461, 24652522, 2528, 2533, 2537, 2548, 2555, 2600, 2615, 2660, 26692726, 2733, 2741, 2786, 2821, 2840, 2844, 2861, 2882, 29042935, 2954, 2968, 2996, 3011, 3027, 3044, 3053, 3066, 31023106, 3110, 3147, 3169, 3189, 3202, 3245, 3317, 3343, 33623381, 3413, 3417, 3431, 3436, 3452, 3459, 3463, 3479, 34843488, 3508, 3522, 3528, 3538, 3542, 3555, 3605, 3640, 3649

3764, 3768, 3792, 3800, 3809, 3817, 3821, 3825, 3830

M.CviPI (299) gc 4, 15, 22, 32, 40, 54, 57, 61, 86, 9097, 104, 121, 134, 143, 157, 164, 189, 216, 233238, 241, 251, 254, 264, 295, 332, 350, 355, 358373, 377, 381, 389, 397, 401, 410, 426, 431, 440450, 460, 463, 472, 477, 494, 499, 507, 516, 520528, 534, 543, 564, 573, 577, 591, 605, 630, 638686, 692, 712, 818, 828, 848, 870, 892, 900, 903913, 929, 937, 948, 969, 977, 998, 1002, 1015, 10221026, 1033, 1042, 1048, 1058, 1073, 1077, 1090, 1094, 11001123, 1128, 1145, 1153, 1156, 1171, 1218, 1240, 1244, 12561259, 1273, 1281, 1292, 1308, 1311, 1319, 1322, 1333, 13401348, 1385, 1399, 1409, 1412, 1417, 1426, 1430, 1440, 14491454, 1459, 1478,

1492, 1495, 1503, 1510, 1516, 1541, 15471568, 1574, 1579, 1582, 1585, 1588, 1594, 1604, 1611, 16281638, 1645, 1649, 1667, 1702, 1711, 1715, 1721, 1733, 17381745, 1749, 1753, 1761, 1785, 1790, 1794, 1798, 1821, 18241848, 1852, 1862, 1869, 1883, 1889, 1904, 1912, 1917, 19201928, 1935, 1945, 1958, 1961, 1972, 1975, 1982, 1992, 20562074, 2077, 2084, 2098, 2112, 2131, 2149, 2183, 2207, 22342250, 2258, 2281, 2286, 2292, 2303, 2306, 2358, 2372, 23932398, 2401, 2425, 2428, 2466, 2473, 2481, 2507, 2529, 25392556, 2571, 2578, 2584, 2595, 2612, 2629, 2637, 2662, 26742688, 2695, 2704, 2708, 2719, 2734, 2742, 2753, 2764, 2767

2775, 2787, 2827, 2857, 2869, 2883, 3028, 3081, 3092, 30953099, 3117, 3124, 3138, 3150, 3179, 3185, 3190, 3222, 32333256, 3261, 3274, 3279, 3298, 3301, 3304, 3311, 3364, 33693377, 3395, 3402, 3407, 3414, 3447, 3453, 3464, 3471, 34903509, 3512, 3531, 3543, 3559, 3590, 3606, 3623, 3636, 36413655, 3659, 3664, 3667, 3685, 3692, 3696, 3703, 3721, 37573769, 3778, 3782, 3785, 3788, 3801, 3806, 3818, 3826

M.SssI (256) cg 5, 14, 21, 31, 35, 42, 64, 76, 87, 98103, 162, 167, 190, 232, 263, 278, 297, 300, 310313, 333, 349, 372, 382, 390, 400, 405, 409, 427441, 449, 461, 464, 473, 478, 493, 500, 506, 521529, 533, 542, 563, 568, 572, 575, 585, 600, 621636, 644, 678, 691, 703, 711, 721, 733, 787, 820

826, 869, 893, 914, 921, 936, 944, 956, 997, 10031043, 1059, 1078, 1083, 1101, 1155, 1172, 1212, 1217, 12431254, 1258, 1279, 1306, 1321, 1324, 1331, 1341, 1349, 13531372, 1381, 1405, 1413, 1422, 1434, 1437, 1441, 1450, 14621465, 1479, 1504, 1512, 1549, 1580, 1583, 1593, 1602, 16181629, 1637, 1642, 1646, 1652, 1658, 1669, 1678, 1696, 17141723, 1732, 1746, 1756, 1759, 1762, 1771, 1774, 1786, 18001823, 1840, 1850, 1863, 1868, 1884, 1895, 1918, 1921, 19341942, 1951, 1957, 1960, 1966, 1971, 1976, 1981, 1991, 20022050, 2055, 2075, 2111, 2152, 2166, 2184, 2206, 2211, 22182259, 2272, 2280, 2283, 2298, 2302, 2305, 2366, 2426, 2447

2451, 2459, 2472, 2506, 2515, 2525, 2550, 2553, 2557, 25672591, 2596, 2613, 2635, 2663, 2689, 2693, 2702, 2736, 27432752, 2794, 2799, 2813, 2816, 2842, 2856, 3007, 3017, 30293039, 3082, 3116, 3125, 3140, 3167, 3191, 3224, 3260, 32733312, 3320, 3330, 3346, 3356, 3365, 3370, 3374, 3383, 33923415, 3419, 3429, 3448, 3465, 3470, 3477, 3491, 3503, 35103519, 3532, 3536, 3558, 3582, 3589, 3607, 3610, 3626, 36373654, 3663, 3676, 3722, 3748, 3756, 3777, 3781, 3784, 37903794, 3798, 3802, 3807, 3819, 3827

M.CviAIV (254) rgcb 7, 23, 41, 58, 122, 144, 165, 217, 234, 239255, 265, 296, 351, 374, 398, 411, 432, 453, 508523, 531, 544, 565, 574, 592, 606, 687, 693, 713

829, 849, 901, 930, 970, 978, 1023, 1034, 1049, 10951103, 1124, 1146, 1157, 1257, 1274, 1282, 1309, 1323, 13341351, 1418, 1427, 1455, 1493, 1569, 1586, 1595, 1605, 16681712, 1718, 1734, 1739, 1822, 1849, 1870, 1890, 1913, 19291936, 1959, 1983, 1993, 2057, 2113, 2208, 2282, 2304, 24292467, 2474, 2508, 2530, 2540, 2630, 2691, 2696, 2705, 27352776, 2788, 2858, 3118, 3151, 3180, 3223, 3234, 3262, 32753299, 3378, 3403, 3408, 3454, 3467, 3472, 3544, 3560, 35913642, 3656, 3668, 3686, 3697, 3758, 3770, 3779, 3789, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239255, 296, 333, 378, 390, 398, 432, 441, 461, 473478, 500, 521, 529, 578, 592, 606, 687, 829, 849

893, 901, 914, 930, 970, 978, 1003, 1023, 1034, 10431049, 1059, 1078, 1095, 1101, 1124, 1146, 1172, 1257, 12741282, 1309, 1334, 1341, 1349, 1413, 1418, 1427, 1441, 14551479, 1493, 1504, 1569, 1583, 1605, 1629, 1646, 1668, 17121722, 1739, 1746, 1762, 1786, 1822, 1849, 1863, 1884, 18901913, 1918, 1929, 1976, 2184, 2259, 2429, 2467, 2530, 25402557, 2613, 2630, 2663, 2696, 2705, 2735, 2743, 2776, 27883029, 3125, 3151, 3180, 3191, 3223, 3234, 3299, 3312, 33653370, 3378, 3403, 3408, 3415, 3448, 3454, 3465, 3491, 35103532, 3544, 3607, 3637, 3642, 3668, 3686, 3697, 3770, 37893802, 3807, 3819, 3827

M.CviJI (254) rgcb 7, 23, 41, 58, 122, 144, 165, 217, 234, 239

255, 265, 296, 351, 374, 398, 411, 432, 453, 508523, 531, 544, 565, 574, 592, 606, 687, 693, 713829, 849, 901, 930, 970, 978, 1023, 1034, 1049, 10951103, 1124, 1146, 1157, 1257, 1274, 1282, 1309, 1323, 13341351, 1418, 1427, 1455, 1493, 1569, 1586, 1595, 1605, 16681712, 1718, 1734, 1739, 1822, 1849, 1870, 1890, 1913, 19291936, 1959, 1983, 1993, 2057, 2113, 2208, 2282, 2304, 24292467, 2474, 2508, 2530, 2540, 2630, 2691, 2696, 2705, 27352776, 2788, 2858, 3118, 3151, 3180, 3223, 3234, 3262, 32753299, 3378, 3403, 3408, 3454, 3467, 3472, 3544, 3560, 35913642, 3656, 3668, 3686, 3697, 3758, 3770, 3779, 3789, 541, 58, 87, 98, 122, 144, 165, 190, 217, 239

255, 296, 333, 378, 390, 398, 432, 441, 461, 473478, 500, 521, 529, 578, 592, 606, 687, 829, 849893, 901, 914, 930, 970, 978, 1003, 1023, 1034, 10431049, 1059, 1078, 1095, 1101, 1124, 1146, 1172, 1257, 12741282, 1309, 1334, 1341, 1349, 1413, 1418, 1427, 1441, 14551479, 1493, 1504, 1569, 1583, 1605, 1629, 1646, 1668, 17121722, 1739, 1746, 1762, 1786, 1822, 1849, 1863, 1884, 18901913, 1918, 1929, 1976, 2184, 2259, 2429, 2467, 2530, 25402557, 2613, 2630, 2663, 2696, 2705, 2735, 2743, 2776, 27883029, 3125, 3151, 3180, 3191, 3223, 3234, 3299, 3312, 33653370, 3378, 3403, 3408, 3415, 3448, 3454, 3465, 3491, 35103532, 3544, 3607, 3637, 3642, 3668,

3686, 3697, 3770, 37893802, 3807, 3819, 3827

M.HindI (96) cac 94, 114, 176, 186, 273, 315, 482, 487, 502, 629668, 683, 746, 947, 1030, 1266, 1326, 1384, 1424, 14581509, 1776, 1812, 1846, 1858, 1897, 1944, 2052, 2117, 21482156, 2256, 2397, 2415, 2559, 2739, 2745, 2868, 2966, 30093128, 3218, 3296, 3309, 3322, 3394, 3450, 3619, 3740, 37663815, 63, 125, 136, 150, 204, 291, 360, 403, 512567, 640, 677, 732, 773, 920, 1242, 1294, 1433, 14721536, 1623, 1651, 2102, 2151, 2236, 2312, 2343, 2350, 24222483, 2563, 2682, 2769, 2812, 2874, 3023, 3114, 3236, 32583397, 3427, 3469, 3535, 3593, 3707

CviJI (75) rgcy 41, 58, 122, 144, 165, 217, 239, 255, 296, 378398, 432, 578, 592, 606, 687, 829, 849, 901, 930

970, 978, 1023, 1034, 1049, 1095, 1124, 1146, 1257, 12741282, 1309, 1334, 1418, 1427, 1455, 1493, 1569, 1586, 16051668, 1712, 1722, 1739, 1822, 1849, 1890, 1913, 1929, 24292467, 2530, 2540, 2630, 2696, 2705, 2735, 2776, 2788, 31513180, 3223, 3234, 3299, 3378, 3403, 3408, 3454, 3544, 36423668, 3686, 3697, 3770, 3789

M.CviTI (75) rgcy 41, 58, 122, 144, 165, 217, 239, 255, 296, 378398, 432, 578, 592, 606, 687, 829, 849, 901, 930970, 978, 1023, 1034, 1049, 1095, 1124, 1146, 1257, 12741282, 1309, 1334, 1418, 1427, 1455, 1493, 1569, 1586, 16051668, 1712, 1722, 1739, 1822, 1849, 1890, 1913, 1929, 24292467, 2530, 2540, 2630, 2696, 2705, 2735, 2776, 2788, 3151

3180, 3223, 3234, 3299, 3378, 3403, 3408, 3454, 3544, 36423668, 3686, 3697, 3770, 3789

M.NgoMXV (56) gcchr 40, 61, 90, 134, 143, 295, 332, 381, 686, 9771033, 1058, 1292, 1430, 1440, 1492, 1503, 1738, 1889, 19171928, 2286, 2612, 2708, 2827, 3179, 3233, 3377, 3685, 36969, 236, 376, 400, 434, 519, 533, 831, 932, 10931159, 1353, 1614, 1720, 1736, 1756, 1793, 2431, 2574, 27783264, 3307, 3405, 3562, 3644, 3658

AciI (44) ccgc 88, 191, 462, 476, 915, 1060, 1416, 1480, 1581, 18641919, 2076, 2185, 2262, 2427, 2664, 2755, 3126, 3371, 34923511, 3638, 3666, 3828, 22, 32, 401, 507, 534, 9371156, 1259, 1322, 1824, 1869, 1935, 1961, 2306, 2473, 31173261, 3757, 3778, 3785

BthCI (34) gcngc 57, 241, 254, 358,

431, 463, 477, 499, 903, 11561259, 1311, 1322, 1412, 1454, 1495, 1582, 1588, 1824, 19201961, 1975, 2077, 2306, 2401, 2428, 2767, 3095, 3301, 33693512, 3667, 3785, 3806

Fnu4HI (34) gcngc 57, 241, 254, 358, 431, 463, 477, 499, 903, 11561259, 1311, 1322, 1412, 1454, 1495, 1582, 1588, 1824, 19201961, 1975, 2077, 2306, 2401, 2428, 2767, 3095, 3301, 33693512, 3667, 3785, 3806

HhaI (30) gcgc 6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803

HinP1I (30) gcgc 6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 1747

1977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803

M.HhaI (30) gcgc 6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803

M.HinP1I (30) gcgc 6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803

M.HpyAVIII (30) gcgc 6, 34, 99, 428, 452, 465, 474, 496, 522, 530894, 1004, 1102, 1173, 1342, 1350, 1414, 1451, 1717, 17471977, 2260, 2597, 2690, 3083, 3192, 3366, 3466, 3533, 3803

Sth132I (28) cccg 89, 480, 602, 1439, 1481, 1514, 2285, 2818, 3332, 33763385, 3584, 31, 76, 400, 533, 585, 956, 1353, 1756

1895, 1966, 2211, 2272, 2635, 2794, 3039, 3477

BstKTI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

ChaI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

DpnI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.AacDam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.AlaK2I (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.AvaV (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.AvaVI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.BspKT6I (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.CviAI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 1690

1768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.DpnIIA (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.DpnIIB (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.Eco67Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.EcoKDam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 2972

3050, 3058, 3069, 3144

M.EcoP1Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.EcoT1Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.EcoT2Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.EcoT4Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.EsaLHCI (24) gatc 408, 1164, 1181,

1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.HindDam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.HinHP1Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.Hpy99VI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.HpyAIII (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 1690

1768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.LlaDCHIA (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.LlaDCHIB (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.LlaKR2I (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.MboIA (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 2972

3050, 3058, 3069, 3144

M.MboIB (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.PgiI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.PhiCh1I (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.Sau3AI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.SmaII (24) gatc 408, 1164, 1181,

1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.Sth368I (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.Sty1344Dam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.StyDam (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

M.TpaI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 1690

1768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

MboI (24) gatc 408, 1164, 1181, 1187, 1192, 1211, 1522, 1600, 1681, 16901768, 2139, 2175, 2192, 2450, 2496, 2514, 2855, 2960, 29723050, 3058, 3069, 3144

Cac8I (23) gcnngc 61, 90, 377, 401, 520, 534, 577, 1002, 1026, 10771094, 1244, 1430, 1649, 1715, 1749, 1794, 1852, 2708, 30993659, 3696, 3782

AluI (22) agct 58, 122, 217, 239, 255, 378, 592, 849, 970, 10231049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770

HpyF10VI (22) gcnnnnnnngc 15, 99, 143, 410, 440, 472, 516, 543, 573, 9031042, 1342, 1426, 1449, 1588, 1711, 1747, 1794, 2704, 30923664, 3778

M.AluI (22) agct 58, 122, 217, 239, 255, 378, 592, 849, 970, 1023

1049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770

M.EsaBC1I (22) agct 58, 122, 217, 239, 255, 378, 592, 849, 970, 10231049, 1095, 1455, 1913, 2467, 2530, 2630, 3151, 3408, 34543544, 3770

MwoI (22) gcnnnnnnngc 15, 99, 143, 410, 440, 472, 516, 543, 573, 9031042, 1342, 1426, 1449, 1588, 1711, 1747, 1794, 2704, 30923664, 3778

HpaII (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

M.BsuFI (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

M.CboI (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 3357

3504M.HapII (21) ccgg 163, 576, 957, 1084,

1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

M.HpaII (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

M.MjaVI (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

M.MspI (21) ccgg 163, 576, 957, 1084, 1255, 1332, 1354, 1382, 1513, 16031670, 1851, 2284, 2526, 2636, 2703, 2737, 3141, 3331, 33573504

BbvI (20) gcagc 241, 903, 1311, 1495, 1588, 2401, 3095, 3301, 57, 254358, 431, 499, 1412, 1454, 1975, 2767, 3369, 3788, 3806

Hpy188III (20) tcnnga 77, 316, 985, 1185, 1196, 1369, 1685, 1694, 1777, 18331877, 1967, 2000,

2732, 2991, 3065, 3148, 3246, 3344, 3478

M.BbvI (20) gcagc 241, 903, 1311, 1495, 1588, 2401, 3095, 3301, 57, 254358, 431, 499, 1412, 1454, 1975, 2767, 3369, 3788, 3806

M.BbvSI (20) gcwgc 57, 241, 254, 358, 431, 499, 903, 1311, 1412, 14541495, 1588, 1975, 2401, 2767, 3095, 3301, 3369, 3788, 3806

TseI (20) gcwgc 57, 241, 254, 358, 431, 499, 903, 1311, 1412, 14541495, 1588, 1975, 2401, 2767, 3095, 3301, 3369, 3788, 3806

BsrI (18) actgg 933, 2347, 3295, 3308, 81, 294, 320, 765, 975, 10561140, 1290, 1491, 2172, 2611, 2654, 2772, 3178

M.EsaDix4I (18) ttaa 51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005

M.EsaDix5I (18) ttaa 51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005

M.MseI (18) ttaa 51, 110, 247, 346, 470, 741, 839, 856, 867, 879

2242, 2607, 2646, 2881, 2934, 2948, 2953, 3005

MseI (18) ttaa 51, 110, 247, 346, 470, 741, 839, 856, 867, 8792242, 2607, 2646, 2881, 2934, 2948, 2953, 3005

M.Hpy99VA (17) cctc 396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596

M.MnlIA (17) cctc 396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596

M.MnlIB (17) cctc 396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596

MnlI (17) cctc 396, 1208, 1272, 1408, 1765, 2455, 2661, 3539, 3822, 25643, 1106, 1941, 2791, 2872, 3272, 3596

HaeIII (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.AvaVII (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.BflBF4I (16) gcsgc 463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785

M.BspRI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.BsuRI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.CglASI (16) gcsgc 463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785

M.HaeIII (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.MthTI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.NgoGII (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.NgoPII (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.Phi3TI (16) ggcc 41, 296, 398, 687,

829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.Rho11sI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.SPBetaI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

M.SPRI (16) ggcc 41, 296, 398, 687, 829, 1257, 1431, 1822, 1849, 24292696, 2776, 3234, 3668, 3686, 3697

TauI (16) gcsgc 463, 477, 1156, 1259, 1322, 1417, 1582, 1824, 1920, 19612077, 2306, 2428, 3512, 3667, 3785

HpyCH4V (15) tgca 55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396

M.CviRI (15) tgca 55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396

M.CviSI (15) tgca 55, 356, 1027, 1129, 1241, 1400, 1575, 1589, 2150, 23942482, 2675, 2765, 3100, 3396

AlwI (14) ggatc 1601, 2193, 2973, 3070, 1164, 1181, 1211, 1522, 1768, 2175

2496, 2960, 3058, 3144

Hpy188I (14) tcnga 622, 1167, 1190, 1316, 1660, 2013, 2322, 2442, 2453, 28993034, 3168, 3521, 3599

M.AlwI (14) ggatc 1601, 2193, 2973, 3070, 1164, 1181, 1211, 1522, 1768, 21752496, 2960, 3058, 3144

M.Hpy188I (14) tcnga 622, 1167, 1190, 1316, 1660, 2013, 2322, 2442, 2453, 28993034, 3168, 3521, 3599

MaeIII (14) gtnac 317, 337, 492, 504, 1472, 1778, 2162, 2350, 2503, 25612892, 3175, 3291, 3354

CviAII (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

FatI (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

HpyCH4I (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.CviAII (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 2489

2499, 2990, 3710M.CviQVII (13) catg 226, 1040, 1221,

1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.CviSII (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.EsaBS1I (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.Hpy166IV (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.Hpy188II (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.Hpy99X (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.HpyAI (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.HpyI (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.NgoMV (13) ggnncc 137, 608, 620, 641, 1103, 1351, 1386, 2524, 2735, 2776

2870, 3642, 3681M.NlaIII (13) catg 226, 1040, 1221,

1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

M.Sse9I (13) aatt 108, 183, 200, 261, 288, 864, 875, 886, 1088, 20162389, 2644, 2950

NlaIII (13) catg 226, 1040, 1221, 1566, 1752, 1783, 1809, 2375, 2411, 24892499, 2990, 3710

NlaIV (13) ggnncc 137, 608, 620, 641, 1103, 1351, 1386, 2524, 2735, 27762870, 3642, 3681

Tsp509I (13) aatt 108, 183, 200, 261, 288, 864, 875, 886, 1088, 20162389, 2644, 2950

BstUI (12) cgcg 33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665

M.BepI (12) cgcg 33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665

M.BsuEII (12) cgcg 33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665

M.DsaV (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

M.Ecl18kI (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

M.FnuDII (12) cgcg 33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665

M.NlaX (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

M.SenPI (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

M.SsoII (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

M.TspRI (12) castg 94, 293, 1030, 1474, 2397, 2424, 2771, 2876, 3025, 32963309, 3815

ScrFI (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

SelI (12) cgcg 33, 451, 475, 495, 871, 1415, 1716, 1748, 2261, 27543084, 3665

StyD4I (12) ccngg 142, 331, 957, 1354, 1514, 1737, 2285, 2636, 3332, 35503563, 3684

TspRI (12) castg 94, 293, 1030, 1474, 2397, 2424, 2771, 2876, 3025, 32963309, 3815

HpyCH4III (11) acngt 423, 705, 946, 1729, 1845, 1953, 2380, 2895, 3208, 36783749

HpyCH4IV (11) acgt 311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008

M.Hpy99XI (11) acgt 311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008

M.HpyAIIA (11) gaaga 550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970

M.HpyCH4IV (11) acgt 311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008

M.MboIIA (11) gaaga 550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970

MboII (11) gaaga 550, 2010, 3061, 3213, 394, 1700, 1910, 2028, 2137, 22152970

TaiI (11) acgt 311, 569, 679, 722, 734, 922, 1466, 1653, 2219, 25923008

BccI (10) ccatc 672, 1144, 1170, 1235, 1663, 2371, 2658, 2782, 690, 1563

BscAI (10) gcatc 1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361

BstF5I (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

BstYI (10) rgatcy 1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070

FokI (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

HinfI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

HpyAV (10) ccttc 1485, 2463, 3171, 3481, 415, 555, 1986, 1996, 2088, 2700

M.BabI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.BspST5I (10) gcatc 1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361

M.BstF5IA (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

M.BstF5ID (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

M.BstYI (10) rgatcy 1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070

M.CcrMI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.CviBI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.CviQVI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.FokI (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

M.HhaII (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.HinfI (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.Hpy99IX (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.HpyAIV (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.SscL1I (10) gantc 46, 277, 729, 751, 1836, 1970, 2823, 3340, 3736, 3811

M.StsI (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

M.XhoII (10) rgatcy 1165, 1188, 1523, 1769, 2176, 2193, 2961, 2973, 3059, 3070

SfaNI (10) gcatc 1319, 1574, 1790, 2131, 2571, 3623, 1641, 1705, 1980, 2361

StsI (10) ggatg 364, 1533, 960, 1143, 1205, 1662, 1687, 2370, 2657, 2838

BslI (9) ccnnnnnnngg 39, 462, 788, 1332, 1876, 3232, 3511, 3677, 3695

Bsp1286I (9) gdgchc 607, 1294, 1387, 1461, 1651, 1713, 2151, 2236, 3397

Hpy99I (9) cgwcg 300, 313, 1437, 1465, 1759, 1774, 2553, 2816, 3610

EsaBC3I (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.CviBIII (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.CviQIII (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.CviSIII (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.EsaBC3I (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.EsaDix3I (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.HpyAX (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.Phi3TII (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.TaqI (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

M.TthHB8I (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

TaqI (8) tcga 645, 1463, 1619, 1643, 1679, 1841, 2167, 3611

BsaJI (7) ccnngg 142, 331, 957, 1161, 1515, 1784, 3551

CdiI (7) catcg 1789, 2570, 691, 1637, 1840, 1981, 2166

EaeI (7) yggccr 42, 297, 1258, 1432, 1823, 1850, 2430

FmuI (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

GdiII (7) cggccr 42, 297, 1258, 1823, 1850, 1258, 2430

Hpy8I (7) gtnnac 677, 732, 749, 1137, 2151, 2909, 3397

M.Cfr13I (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

M.CfrI (7) yggccr 42, 297, 1258, 1432, 1823, 1850, 2430

M.EaeI (7) yggccr 42, 297, 1258, 1432, 1823, 1850, 2430

M.EcoHK31I (7) yggccr 42, 297, 1258, 1432, 1823, 1850, 2430

M.Hpy8I (7) gtnnac 677, 732, 749, 1137, 2151, 2909, 3397

M.HpyAIX (7) gtnnac 677, 732, 749, 1137, 2151, 2909, 3397

M.PspPI (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

M.Sau96I (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

MspA1I (7) cmgckg 59, 379, 1096, 1456, 2185, 3126, 3371

Sau96I (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

TspDTI (7) atgaa 48, 1838, 2942, 962, 1395, 2537, 2840

UnbI (7) ggncc 399, 687, 1867, 2458, 2680, 2697, 2776

BanI (6) ggyrcc 137, 641, 1103, 1351, 1386, 2870

BsiEI (6) cgrycg 409, 1258, 2302, 2451, 3374, 3798

BstNI (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

CjeI (6) ccannnnnngt 3129, 3684, 383, 3030, 3130, 3208

EcoHI (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

FauI (6) cccgc 89, 480, 1481, 32, 401, 534

HaeII (6) rgcgcy 7, 523, 531, 1103, 1351, 3467

Hin4I (6) gaynnnnnvtc 1529, 1559, 2749, 2823, 1529, 2823

HphI (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

LpnI (6) rgcgcy 7, 523, 531, 1103, 1351, 3467

M.BcnIA (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

M.BcnIB (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

M.BstNI (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

M.Eco1831I (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

M.EcoHI (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

M.EcoKDcm (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

M.EcoPI (6) agacc 791, 1673, 2750, 1371, 2901, 3036

M.EcoRII (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

M.HgiCI (6) ggyrcc 137, 641, 1103, 1351, 1386, 2870

M.HphIA (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

M.HphIB (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

M.Hpy99II (6) gtsac 317, 504, 1472, 1778, 2350, 2561

M.MlyI (6) gastc 277, 729, 751, 2823, 3340, 3811

M.MvaI (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

M.NgoBVIIIA (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

M.NgoHVIII (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

M.StyLTI (6) cagag 1108, 1315, 3252, 3278, 3598, 3189

M.Tsp45I (6) gtsac 317, 504, 1472, 1778, 2350, 2561

MlyI (6) gagtc 751, 3340, 277, 729, 2823, 3811

NciI (6) ccsgg 957, 1354, 1514, 2285, 2636, 3332

PleI (6) gagtc 751, 3340, 277, 729, 2823, 3811

PspGI (6) ccwgg 142, 331, 1737, 3550, 3563, 3684

SfcI (6) ctryag 271, 459, 1401, 2577, 3255, 3446

SimI (6) gggtc 648, 954, 1780, 3037, 2749, 3518

SmlI (6) ctyrag 248, 1743, 2198, 3066, 3343, 3605

SspD5I (6) ggtga 2103, 2344, 669, 2118, 2740, 2967

Tsp45I (6) gtsac 317, 504, 1472, 1778, 2350, 2561

BceAI (5) acggc 298, 1325, 3225, 638, 1761

BcefI (5) acggc 298, 1325, 3225, 638, 1761

BfaI (5) ctag 527, 1051, 2628, 2963, 3216

BmrI (5) actggg 321, 976, 1057, 1291, 2773

BpuEI (5) cttgag 1743, 3343, 2198, 3066, 3605

BsaWI (5) wccggw 1085, 1383, 2527, 3358, 3505

BsiHKAI (5) gwgcwc 1461, 1651, 2151, 2236, 3397

Eco57MI (5) ctgrag 3184, 1477, 1909, 2136, 2733

M.BfiIA (5) actggg 321, 976, 1057, 1291, 2773

M.BfiIB (5) actggg 321, 976, 1057, 1291, 2773

M.MthZI (5) ctag 527, 1051, 2628, 2963, 3216

TaqII (5) gaccga 790, 2301, 1373, 2460, 3799

AcuI (4) ctgaag 3184, 1477, 1909, 2136

Bme1580I (4) gkgcmc 1294, 1387, 2151, 3397

BseMII (4) ctcag 2861, 3027, 3436, 2321

BspCNI (4) ctcag 2861, 3027, 3436, 2321

BsrBI (4) ccgctc 191, 536, 1963, 3780BsrFI (4) rccggy 577, 1671, 1852,

2738BtsI (4) gcagtg 2425, 94, 1030, 2397CjePI (4) ccannnnnnntc 3178, 2439, 2911,

3031DdeI (4) ctnag 2321, 2861, 3027,

3436EarI (4) ctcttc 395, 1701, 1911,

2029EciI (4) ggcgga 1865, 2665, 3493,

3639HaeI (4) wggccw 1432, 3235, 3687,

3698HgaI (4) gacgc 3031, 3609, 450,

2281M.AvaIX (4) rccggy 577, 1671, 1852,

2738M.Cfr10I (4) rccggy 577, 1671, 1852,

2738M.Cfr6I (4) cagctg 59, 379, 1096, 1456M.DdeI (4) ctnag 2321, 2861, 3027,

3436M.Eco57IA (4) ctgaag 3184, 1477, 1909,

2136M.Eco57IB (4) ctgaag 3184, 1477, 1909,

2136M.HgaIA (4) gacgc 3031, 3609, 450,

2281M.HgaIB (4) gacgc 3031, 3609, 450,

2281M.PvuII (4) cagctg 59, 379, 1096, 1456M.TfiI (4) gawtc 46, 1836, 1970, 3736MslI (4) caynnnnrtg 1789, 2052, 2411,

2570PvuII (4) cagctg 59, 379, 1096, 1456TfiI (4) gawtc 46, 1836, 1970, 3736Tth111II (4) caarca 1797, 3098, 3137,

3104AceIII (3) cagctc 850, 2531, 3771

ApoI (3) raatty 865, 876, 887AseI (3) attaat 52, 111, 2647AvaII (3) ggwcc 1867, 2458, 2680BsaAI (3) yacgtr 680, 923, 1654BsaHI (3) grcgyc 1103, 1351, 2282BseYI (3) cccagc 1098, 1856, 3407BspMI (3) acctgc 1019, 1248, 1611BsrDI (3) gcaatg 2764, 1578, 2582BssSI (3) cacgag 1944, 2154, 3538DraI (3) tttaaa 2243, 2935, 2954DrdI (3) gacnnnnnngtc 729, 1379, 3609FspI (3) tgcgca 429, 1452, 2598M.BsaAI (3) yacgtr 680, 923, 1654M.DraI (3) tttaaa 2243, 2935, 2954M.EcoP15I (3) cagcag 3094, 3303, 3187M.MmeIA (3) tccrac 726, 3337, 3521M.MmeIB (3) tccrac 726, 3337, 3521M.SinI (3) ggwcc 1867, 2458, 2680M.VspI (3) attaat 52, 111, 2647MmeI (3) tccrac 726, 3337, 3521PpiI (3) gaacnnnnnctc 736, 3020, 2177Psp03I (3) ggwcc 1867, 2458, 2680VpaK11AI (3) ggwcc 1867, 2458, 2680AclI (2) aacgtt 2220, 2593AflIII (2) acrygt 922, 3711AlwNI (2) cagnnnctg 974, 3300ApaLI (2) gtgcac 2151, 3397BanII (2) grgcyc 607, 1713BbeI (2) ggcgcc 1103, 1351BciVI (2) gtatcc 1560, 3502BglI (2) gccnnnnnggc 440, 2704BsaBI (2) gatnnnnatc 1187, 1211BsaXI (2) acnnnnnctcc 734, 28BsmAI (2) gtctc 1199, 2751Csp6I (2) gtac 1655, 2339FalI (2) aagnnnnnctt 245, 1674HaeIV (2) gaynnnnnrtc 1529, 2823KasI (2) ggcgcc 1103, 1351M.AclI (2) aacgtt 2220, 2593M.AflIII (2) acrygt 922, 3711M.Alw26I (2) gtctc 1199, 2751M.BanII (2) grgcyc 607, 1713M.CfrBI (2) ccwwgg 1161, 1784M.CviQI (2) gtac 1655, 2339

M.CviRII (2) gtac 1655, 2339M.HaeIV (2) gaynnnnnrtc 1529, 2823M.NgoBIX (2) gtannnnnctc 2162, 3444M.NgoMIV (2) gccggc 577, 1852M.NspHI (2) rcatgy 1753, 3711M.NspI (2) rcatgy 1753, 3711M.PhiMx8I (2) ctssag 1401, 2733M.RsaI (2) gtac 1655, 2339M.SauLPI (2) gccggc 577, 1852NaeI (2) gccggc 577, 1852NarI (2) ggcgcc 1103, 1351NgoMIV (2) gccggc 577, 1852NspI (2) rcatgy 1753, 3711PvuI (2) cgatcg 409, 2451RsaI (2) gtac 1655, 2339SapI (2) gctcttc 1702, 1912SfoI (2) ggcgcc 1103, 1351StyI (2) ccwwgg 1161, 1784TspGWI (2) acgga 2051, 2368AflII (1) cttaag 248AhdI (1) gacnnnnngtc 2823AloI (1) gaacnnnnnntcc 736ApaBI (1) gcannnnntgc 903BcgI (1) cgannnnnntgc 2302BclI (1) tgatca 1193BglII (1) agatct 1188BpmI (1) ctggag 2733BsaI (1) ggtctc 2751BsmFI (1) gggac 1488Bsp24I (1) gacnnnnnntgg 3031BspHI (1) tcatga 2991BssHII (1) gcgcgc 1749BstAPI (1) gcannnnntgc 903BtgI (1) ccrygg 1784DraIII (1) cacnnngtg 683EagI (1) cggccg 1258M.AloI (1) gaacnnnnnntcc 736M.BcgI (1) cgannnnnntgc 2302M.BclI (1) tgatca 1193M.BglII (1) agatct 1188M.BmeTI (1) tgatca 1193M.BspLU11IIIA (1) gggac 1488M.BspLU11IIIC (1) gggac 1488M.BsuBI (1) ctgcag 1401

M.DraIII (1) cacnnngtg 683M.EcoKI (1) aacnnnnnngtgc 2160M.EcoR124II (1) gaannnnnnnrtcg 1642M.NmeSI (1) agtact 2340M.PstI (1) ctgcag 1401M.ScaI (1) agtact 2340M.StyLTIII (1) gagnnnnnnrtayg 2319M.StySPI (1) aacnnnnnngtrc 2160M.TaeII (1) tgatca 1193M.Tth111I (1) gacnnngtc 1470M.XcmI (1) ccannnnnnnnntgg 1170M.XmaIII (1) cggccg 1258MscI (1) tggcca 1432PciI (1) acatgt 3711PsiI (1) ttataa 808PstI (1) ctgcag 1401RsrII (1) cggwccg 1868ScaI (1) agtact 2340SphI (1) gcatgc 1753TatI (1) wgtacw 2340Tth111I (1) gacnnngtc 1470XcmI (1) ccannnnnnnnntgg 1170XmnI (1) gaannnnttc 2223

Non-CuttersAarI, AatII, Acc65I, AccI, AfeI, AgeI, AleI, ApaI, AscI, AsiSIAvaI, AvrII, BaeI, BamHI, Bbr7I, BbsI, BbvCI, BfrBI, BlpI, BmgBIBmtI, BplI, Bpu10I, BseRI, BsgI, BsiWI, BsmBI, BsmI, BspEI, BsrGIBstBI, BstEII, BstXI, BstZ17I, Bsu36I, ClaI, EcoICRI, EcoNI, EcoO109I, EcoRIEcoRV, FseI, FspAI, HincII, HindIII, HpaI, KpnI, M.AccI, M.ApaI, M.AquIM.BaeI, M.BbvCIA, M.BbvCIB, M.BnaI, M.BseCI, M.Bsp106I, M.BstI, M.BstVI,

M.Bsu15I, M.Cfr9IM.ClaI, M.EcaI, M.EcoAI, M.EcoBI, M.EcoO109I, M.EcoR124I, M.EcoRI,

M.EcoRV, M.Esp3I, M.HincIIM.HindII, M.HindIII, M.HpaI, M.KpnAI, M.KpnBI, M.KpnI, M.MunI, M.NgoAV,

M.PaeR7I, M.PhiBssHIIM.PshAI, M.Rrh4273I, M.RsrI, M.SacI, M.SalI, M.Sau3239I, M.SmaI, M.SnaBI,

M.SsoI, M.XbaIM.XcyI, M.XhoI, MfeI, MluI, NdeI,NcoI, NheI, Nli3877I, NotI, NruI, NsiIPacI, PflMI, PfoI, PmeI, PmlI, Ppu10I, PpuMI, PshAI, PspOMI, PsrIPssI, RleAI, SacI, SacII, SalI, SanDI, SbfI, SciI, SexAI, SfiISgrAI, SmaI, SnaBI, SpeI, SrfI, Sse232I, Sse8647I, SspI, StuI, SwaIUthSI, XbaI, XhoI, XmaI, ZraI